Innovative Modular System for High-Throughput Protein Purification.HANOVER, Germany -- Vivascience AG Launches Vivapure(TM) 8-to-96 Well Cobalt-Chelate Kit for His-tagged(1) Protein Purification Vivascience, a subsidiary of Sartorius AG, introduces the Vivapure(TM) 8-to-96 well cobalt-chelate kit, a complete system for simultaneous purification of multiple His-tagged proteins by Immobilized Metal Affinity Chromatography (IMAC Apple's primary desktop computer. The iMac is an "all-in-one" that houses the computer and drives within the flat panel monitor case. Since their debut in 1998, the iMacs have been very popular. Like all Macintosh models in that time frame, the first iMacs used PowerPC chips. ). The kit includes ready-to-use, membrane-based hardware for all steps of the purification process: lysate ly·sate n. The cellular debris and fluid produced by lysis. filtration, protein purification, eluate eluate /el·u·ate/ (el´u-at) the substance separated out by, or the product of, elution or elutriation. el·u·ate n. The solution of solvent and dissolved matter resulting from elution. collection and storage. All kit components are compatible with multi-channel devices and automated workstations, making the system suitable for high-throughput applications. The Vivapure(TM) 8-to-96 well cobalt-chelate kit offers a unique modular format. Depending on the sample number, up to twelve individual 8-strips are inserted into frames with the footprint of standard 96-well plates. This feature results in proportionate savings when less than 96 samples are simultaneously processed. Prior to protein purification, cell lysates are cleared by filtration through the multi-layer membrane of the Vivaclear(TM) plus 8-strips. Afterwards, His-tagged proteins are isolated from the lysate using the Vivapure(TM) cobalt-chelate 8-strips. Each well of these 8-strips is pre-packed with a membrane containing immobilized cobalt-IDA (iminodiacetic acid) chelates, which specifically capture His-tagged proteins. Along with negligible unspecific Adj. 1. unspecific - not detailed or specific; "a broad rule"; "the broad outlines of the plan"; "felt an unspecific dread" broad general - applying to all or most members of a category or group; "the general public"; "general assistance"; "a general rule"; protein binding, this membrane-based technology assures high and reproducible recoveries of ultrapure target proteins. Parallel processing of multiple samples is accomplished by rapid centrifugation-based steps. Up to 250 ug His-tagged protein per well can be purified within 45 minutes. The kit is ideal for fast screening of expression libraries, optimisation of expression conditions and preparation of multiple purified proteins to be used in interaction studies, structural and functional analyses or for spotting protein arrays. The Vivapure(TM) 8-to-96 well cobalt-chelate kit enlarges the Vivapure family of membrane-based protein purification tools, which include a variety of centrifugal devices using metal chelate chelate Any of a class of coordination or complex compounds consisting of a central atom of a metal (usually a transition element) attached to a large molecule (ligand). , ion exchange ion exchange n. A reversible chemical reaction occurring between an insoluble solid and a solution during which ions may be interchanged, used in the separation of radioactive isotopes. , C18 and Protein-A functional groups as well as the new kits for human serum albumin removal and adenovirus adenovirus Any of a group of spheroidal viruses, made up of DNA wrapped in a protein coat, that cause sore throat and fever in humans, hepatitis in dogs, and several diseases in fowl, mice, cattle, pigs, and monkeys. purification. (1) protected by patents of third parties For further information on Vivadish and Vivascience products for cell culture as well as protein purification, ultrafiltration ultrafiltration /ul·tra·fil·tra·tion/ (ul?trah-fil-tra´shun) filtration through a filter capable of removing very minute (ultramicroscopic) particles. ul·tra·fil·tra·tion n. and filtration products, please visit our website at www.vivascience.com. Vivascience/Sartorius Vivascience AG develops innovative solutions for protein purification and analysis with a product portfolio of device formats for filtration, ultrafiltration, and cell culture. Vivascience is a 100 percent owned subsidiary of Sartorius AG, Goettingen, an internationally leading laboratory and process technology supplier. In 2003, the technology group earned sales revenue of 442.3 million euros and employed a good 3,660 persons. Its biotechnology segment focuses on filtration and separation products, bioreactors and proteomics. The mechatronics (MECHAnics elecTRONICS) The combination of mechanical and electronic systems. Embracing robotics, industrial control systems and human interfaces in numerous disciplines, mechatronics is a major step beyond "electromechanical," in which only electricity is required. segment manufactures weighing equipment and systems for laboratory and industrial applications. |
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