Inhibition of colon cancer with Azadirachta indica.Roy M, Kobori M, Takenaka M, Nakahara K, Shinmoto H, Tsushida T. 2006. Inhibition of colon cancer colon cancer, cancer of any part of the colon (often called the large intestine). Colon cancer is the second most common cancer diagnosed in the United States. (HT-2) cell proliferation by a triterpenoid isolated from Azadirachta indica is accompanied by cell cycle arrest and upregulation of P21. PlantaMed 72:917-23. (CB)HT-29 human colorectal adenocarinoma cells were treated with nimbolide from Azadirachta indica (neem neem (nem) Azadirachta indica, a large evergreen tree having antifungal, antibacterial, antiviral, and antimalarial activity; long used medicinally for a wide variety of indications. ) for 12, 24, 36 and 48h. Nimbolide was found to cause extensive morphological changes to HT-29 cells. More than 50% of cells became detached from the dishes and displayed shrunken shrunk·en v. A past participle of shrink. shrunken Verb a past participle of shrink Adjective reduced in size Adj. 1. morphology when the cells were exposed to 5[micro]M of nimbolide for 12h. These changes were not observed in control cells. Nimbolide also inhibited HT-29 cell growth by 3568% after 12h and 57-89% after 48h. Cells were stained with annexin V and PI to determine the nimbolide-induced apoptotic death of HT-29 cells. Nimbolide caused an increase in apoptosis of 12.2% at treatment doses of 5[micro]M and 14.9% and doses of 10[micro]M after 48h. This study indicates that nimbolide inhibits the growth of colon cancer cells in vitro. Jerome Sarris 3 Widgee Place, Chapel Hill Qld 4069 Cara Barnes 20 Brendon Street, Tarragindi Qld 4121 |
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