Inhibition of colon cancer with Azadirachta indica.
HT-29 human colorectal adenocarinoma cells were treated with nimbolide from Azadirachta indica (neem) for 12, 24, 36 and 48h. Nimbolide was found to cause extensive morphological changes to HT-29 cells. More than 50% of cells became detached from the dishes and displayed shrunken morphology when the cells were exposed to 5[micro]M of nimbolide for 12h. These changes were not observed in control cells.
Nimbolide also inhibited HT-29 cell growth by 3568% after 12h and 57-89% after 48h.
Cells were stained with annexin V and PI to determine the nimbolide-induced apoptotic death of HT-29 cells. Nimbolide caused an increase in apoptosis of 12.2% at treatment doses of 5[micro]M and 14.9% and doses of 10[micro]M after 48h.
This study indicates that nimbolide inhibits the growth of colon cancer cells in vitro.
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|Publication:||Australian Journal of Medical Herbalism|
|Article Type:||Brief article|
|Date:||Dec 22, 2006|
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