Printer Friendly

Inhibition of colon cancer with Azadirachta indica.

Roy M, Kobori M, Takenaka M, Nakahara K, Shinmoto H, Tsushida T. 2006. Inhibition of colon cancer (HT-2) cell proliferation by a triterpenoid isolated from Azadirachta indica is accompanied by cell cycle arrest and upregulation of P21. PlantaMed 72:917-23. (CB)

HT-29 human colorectal adenocarinoma cells were treated with nimbolide from Azadirachta indica (neem) for 12, 24, 36 and 48h. Nimbolide was found to cause extensive morphological changes to HT-29 cells. More than 50% of cells became detached from the dishes and displayed shrunken morphology when the cells were exposed to 5[micro]M of nimbolide for 12h. These changes were not observed in control cells.

Nimbolide also inhibited HT-29 cell growth by 3568% after 12h and 57-89% after 48h.

Cells were stained with annexin V and PI to determine the nimbolide-induced apoptotic death of HT-29 cells. Nimbolide caused an increase in apoptosis of 12.2% at treatment doses of 5[micro]M and 14.9% and doses of 10[micro]M after 48h.

This study indicates that nimbolide inhibits the growth of colon cancer cells in vitro.

Jerome Sarris

3 Widgee Place, Chapel Hill Qld 4069

Cara Barnes

20 Brendon Street, Tarragindi Qld 4121
COPYRIGHT 2006 National Herbalists Association of Australia
No portion of this article can be reproduced without the express written permission from the copyright holder.
Copyright 2006 Gale, Cengage Learning. All rights reserved.

 Reader Opinion

Title:

Comment:



 

Article Details
Printer friendly Cite/link Email Feedback
Publication:Australian Journal of Medical Herbalism
Article Type:Brief article
Geographic Code:8AUST
Date:Dec 22, 2006
Words:195
Previous Article:The pharmacological actions of Withania somnifera.
Next Article:The sedating activity of hops.
Topics:

Terms of use | Copyright © 2014 Farlex, Inc. | Feedback | For webmasters