Inhibition of RLIP76 causes complete regression of melanoma in mice.Singhal SS, Awasthi YC, Awasthi S. 2006. Regression of melanoma in a murine murine /mu·rine/ (mur´en) pertaining to, derived from, or characteristic of mice or rats. mu·rine adj. model by RLIP RLIP Requested Line Item Performance RLIP Readiness Level Implementing Procedure 76 depletion. Cancer Res 66:2354-2360. Studies have shown that inhibition or depletion of RLIP76, a glutathione-conjugate transport protein that helps cells defend themselves against toxicants, causes apoptosis in a number of cancer cell types. Now NIEHS-supported researcher Yogesh C. Awasthi of The University of Texas Medical Branch "UTMB" redirects here. For other system schools, see University of Texas System. The University of Texas Medical Branch (UTMB) is a component of the University of Texas System located in Galveston, Texas, about 50 miles (80 km) southeast of downtown Houston. at Galveston and colleagues have confirmed that inhibition or depletion of RLIP76 causes apoptosis in malignant melanoma Malignant Melanoma Definition Malignant melanoma is a type of cancer arising from the melanocyte cells of the skin. Melanocytes are cells in the skin that produce a pigment called melanin. cells. RLIP76 is implicated im·pli·cate tr.v. im·pli·cat·ed, im·pli·cat·ing, im·pli·cates 1. To involve or connect intimately or incriminatingly: evidence that implicates others in the plot. 2. in the regulation of multiple signaling pathways. The clinical and physiological implications of RLIP76 extend to diverse processes, including stress resistance, chemotherapy drug resistance, radiation resistance, oxidative stress-induced disease, and even insulin resistance Insulin Resistance Definition Insulin resistance is not a disease as such but rather a state or condition in which a person's body tissues have a lowered level of response to insulin, a hormone secreted by the pancreas that helps to regulate the level . The Texas researchers compared the expression of RLIP76 in normal cells and several cancer cell lines to explore potential clinical impacts. Their studies also included techniques to determine whether depletion of RLIP76 would cause cancer-specific apoptosis. Expression of RLIP76 was found to be greater in malignant cells than in nonmalignant cells. Inhibition or depletion of the protein also caused preferential apoptosis in a variety of malignant cells in culture. Most importantly, in a mouse melanoma model, administration of a single dose of RLIP76 antibodies, short interfering RNAs, or antisense antisense, DNA or RNA manipulated in a laboratory so that its components (nucleotides) form a complementary copy of normal, or "sense," messenger RNA (mRNA; see nucleic acid). oligonucleotides caused complete tumor regression in 10 days. These findings provide strong evidence that inhibition of RLIP76 through genetic engineering or by administration of antibodies may be a clinically relevant approach to treating cancer, especially melanoma. The dramatic results suggest advancing this technique to clinical practice. Further studies in melanoma and other cancer models and other susceptible cancer cell lines would be needed to show the general applicability of these results prior to human clinical applications. |
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