Hughes denies he is father of love-child.
Ms Sharlene Gillies, of Wallace Close, Lion Farm estate, Oldbury, claimed the 23-year-old West Bromwich Albion striker made her pregnant after they made love at his home in Smethwick.
Ms Gillies, aged 20, has now named Hughes as the father of her nine-week-old daughter Paige and is seeking maintenance from him.
But Hughes said in a statement yesterday that he was very upset about the allegations and denied even having sex with Ms Gillies.
Hughes' agent Dale Wright said that the player admitted having a "sexual encounter" with Ms Gillies, but denied that intercourse took place.
"Lee is extremely upset by these claims and denies categorically that this child is his," said Mr Wright.
"He admits he did have an encounter with this woman, but says they did not have full sex and the baby cannot be his.
"Lee is willing to have DNA tests to prove that he is not the father of this child.
"If Lee was shown to be the father then he will face up to his responsibilities," added Mr Wright.
The allegations could not have come at a worse time for Hughes, who is due to marry 20-year-old lap dancer Miss Donna Nisbet.
Ms Gillies, who lives in a flat in Selby House, said she was prepared to go to court to win maintenance from Hughes, who earns more than pounds 6,000 a week and was English football's top scorer last season with 32 goals.
Ms Gillies is believed to be on holiday and could not be contacted for further comment.