Glycomics; methods and protocols.

9781588297747

Glycomics; methods and protocols.

Ed. by Nicolle H. Packer & Niclas G. Karlsson.

Humana Press Inc.

2009

389 pages

$110.00

Hardcover

Methods in molecular biology, 534

QP601

Glycomics is the analysis of the structure and functions of glycans or oligosaccharides (chains of sugars) attached to biomolecules and includes the analysis of glycoproteins, proteoglycans, glycolipids, peptidoglycans, and lipopolysacchrarides. Packer (Macquarie U., Australia) and Karlsson (National U. of Ireland) present 26 chapters providing step-by-step descriptions of methods and protocols of glycomics. They have organized the contributions into sections concentrating on glycoprotein and proteoglycan analysis; glycosylation site determination methods; the use of bioinformatic tools for analysis of mass spectrometric data on glycans; analysis of the specific single O-GlcNAc molecule found on nuclear and cytoplasmic proteins; and methods for measuring glycan biomolecular interactions using glycan arrays, mass spectrometry, nuclear magnetic resonance, antibodies, and small molecule inhibitors. As with the rest of the series, contributions adopt a uniform format that includes an introduction to the topic, a list of necessary materials and reagents, step-by-step protocols, and notes on troubleshooting and known pitfalls.

([c]2009 Book News, Inc., Portland, OR)