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Gain insight into survival, growth of E. sakazakii in infant cereals.


E. sakazakii infections in infants and children up to four years of age have been associated with their consumption of infant formulas Infant formula is an artificial substitute for human breast milk. Formulas are designed for infant consumption, and are usually based on either cow milk or soy milk. Use of infant formula has been decreasing in industrial countries for over forty years as a result of antenatal . The pathogen's ability to survive nad grow in infnat cereals as affected by water activity (aW), temperature nad reconstitution liquid is unknown.

University of Georgia Organization
The President of the University of Georgia (as of 2007, Michael F. Adams) is the head administrator and is appointed and overseen by the Georgia Board of Regents.
 scientists undertook a study to determine the survival characteristics of E. sakazakii at 4 C to 30 C in infant cereal cereal
 or grain

Any grass yielding starchy seeds suitable for food. The most commonly cultivated cereals are wheat, rice, rye, oats, barley, corn, and sorghum. As human food, cereals are usually marketed in raw grain form or as ingredients of food products.
 at aW 0.23 to 0.88. Their results provide new insights into the behavior of E. sakazakii as affected by conditions under which dry and reconstituted cereals are held during commercial distribution and in hospital and home settings. This information is helpful when assessing and managing the risk of E. sakazakii infection associated with infant cereals.

The scientists inoculated rice, barley barley, annual cereal plant (Hordeum vulgare and sometimes other species) of the family Gramineae (grass family), cultivated by humans probably as early as any cereal.  and mixed-grain infant cereals at aW 0.23 to 0.88 with 5.3 log CFU CFU

see colony-forming units.
 per g of E. sakazakii and held them at 4 C, 21 C and 30 C. Populations were monitored for up to six months.

Rice, rice with banana, and oatmeal infant cereals were reconstituted with water, milk or apple juice, inoculated with E. sakazakii at 0.55 CFU per ml, and held at 4 C, 12 C, 21 C and 30 C for up to 72 hours. Populations were determined at 4-hour to 24-hour intervals. The investigators correlated cor·re·late  
v. cor·re·lat·ed, cor·re·lat·ing, cor·re·lates

v.tr.
1. To put or bring into causal, complementary, parallel, or reciprocal relation.

2.
 the rate of decrease in viable E. sakazakii levels in dry cereals with increases in water activity and storage temperature.

The bacteria did not grow in reconstituted cereals stored at 4 C or in cereals reconstituted with apple juice and stored at 12 C and 21 C. Growth occurred in cereals reconstituted with water or milk and stored at 12 C, 21 C and 30 C for 48 hours, 12 hours and 8 hours, respectively. After reaching 8 to 9 log CFU per mL of reconstituted cereal, populations decreased, concurrent with decreases in pH levels.

Further information. Larry Beuchat, Center for Food Safety, Department of Food Science and Technology, 1109 Experiment St., Melton mel·ton  
n.
A heavy woolen cloth used chiefly for making overcoats and hunting jackets.



[After Melton Mowbray, an urban district of central England.]
 Building, University of Georgia, Griffin, GA 30223; phone: 770-412-4740; fax: 770-229-3216; email: lbeuchat@uga.edu.
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Publication:Microbial Update International
Date:Feb 1, 2007
Words:352
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