Fast and Specific HSA and IgG Depletion; Vivascience AG Introduces Vivapure Anti-HSA/IgG Kit for Improved Biomarker Detection.

HANOVER, Germany -- Vivascience, a subsidiary of Sartorius AG, further expands the Vivapure sample preparation product line by introducing a new human albumin and IgG depletion kit.

The Vivapure Anti-HSA/IgG Kit utilizes a combination of a unique Anti-HSA antibody fragment and Protein G resin for specifically depleting albumin and IgG from human samples.

The kit provides (more than)95 % removal of albumin and IgG from 20 ul of human serum along with low non-specific binding of potential serum biomarkers. Albumin and IgG depleted samples are recovered in a low salt buffer, facilitating downstream applications. The method is quick, suitable for parallel processing and offered in a ready-to-use kit.

With this kit, Vivascience expands its successful range of Vivapure sample preparation products: Vivapure Anti-HSA Kit for Albumin Depletion, Vivapure Anti-HSA Resin for Albumin Depletion from serum volumes up to 400 ul, and Vivapure Ion Exchange spin columns for sample fractionation.

The new Vivapure Kit further improves the resolution and detection of lower abundance sample proteins by electrophoresis, chromatography, and mass spectrometry by removing up to 75% of high abundant serum proteins.

The Anti-HSA ligand also shows reactivity against rat and mouse albumin. As Protein G is also efficient at binding rat and mouse IgG, this kit can be utilized for processing samples from the three most frequently encountered sample sources in biomarker research.

The Vivapure Anti-HSA/IgG Kit contains Anti-HSA/IgG Affinity Resin, centrifugal columns which can individually be filled with the resin amount necessary, micro-centrifuge tubes, and binding buffer for preparing 12 serum samples. The spin column format allows the processing of a single sample within 20 minutes and is just as convenient for the parallel processing of several samples simultaneously using standard laboratory equipment.

For further information on Vivapure Anti-HSA/IgG and Vivascience products for cell culture as well as protein purification, ultrafiltration and filtration products, please visit our website at www.vivascience.com.

Vivascience/Sartorius

Vivascience AG develops innovative solutions for protein purification and analysis with a product portfolio of device formats for filtration, ultrafiltration, and cell culture. Vivascience is a 100 percent owned subsidiary of Sartorius AG, Goettingen, an internationally leading laboratory and process technology supplier. In 2003, the technology group earned sales revenue of 442.3 million euros and employed a good 3,660 persons. Its biotechnology segment focuses on filtration and separation products, bioreactors and proteomics. The mechatronics segment manufactures weighing equipment and systems for laboratory and industrial applications.