Extended-spectrum [beta]-lactamase--producing Enterobacteriaceae, Central African Republic.To the Editor: Since the early 1980s, extended-spectrum [beta]-lactamases (ESBLs) have been the largest source of resistance to broad-spectrum oxyimino-cephalosporins among Enterobacteriaceae (1). Molecular analysis techniques suggest that many ESBLs are derived from mutations in TEM-1, TEM-2, and SHV-1 [beta]-lactamases and that these ESBLs can hydrolyze hydrolyze
to performance hydrolysis. the extended-spectrum cephalosporins Cephalosporins Definition
Cephalosporins are medicines that kill bacteria or prevent their growth.
Cephalosporins are used to treat infections in different parts of the body—the ears, nose, throat, lungs, sinuses, and (particularly ceftazidime) and aztreonam (1).
Members of a new group of ESBLs have been recently identified (1). Among them, CTX-M-type ESBLs are rapidly expanding and are derived from chromosomal class A [beta]-lactamases of Kluyvera spp. (1,2). The CTX-M enzymes are not related to TEM TEM
1. transmission electron microscope.
3. transmissible encephalopathy of mink. or SHV enzymes, as they share only 40% identity with these ESBLs (2). These ESBLS are usually characterized by a higher level of resistance to cefotaxime than ceftazidime, except for CTX-M-19 (2). Most organisms that harbor ESBLs are also resistant to other classes of antimicrobial drugs, such as aminoglycosides, fluoroquinolones, chloramphenicol chloramphenicol (klōr'ămfĕn`əkŏl'), antibiotic effective against a wide range of gram-negative and gram-positive bacteria (see Gram's stain). It was originally isolated from a species of Streptomyces bacteria. , and tetracyclines Tetracyclines Definition
Tetracyclines are medicines that kill certain infection-causing microorganisms.
Tetracyclines are called "broad-spectrum" antibiotics, because they can be used to treat a wide variety of (1,2).
Reports concerning the existence of ESBL-producing Enterobacteriaceae in sub-Saharan Africa are scarce. We therefore conducted a study in the Central African Republic Central African Republic, republic (2005 est. pop. 3,800,000), 240,534 sq mi (622,983 sq km), central Africa. The landlocked nation is bordered by Chad (N), Sudan (E), Congo (Kinshasa) and Congo (Brazzaville) (S), and Cameroon (W). to determine the frequency of ESBLs in Enterobacteriaceae isolated at the Institut Pasteur de Bangui and to characterize their [bla.sub.TEM], [bla.sub.SHV], and [bla.sub.CTX-M] genes.
From January 2003 to March 2005, all Enterobacteriaceae isolated from human specimens at the Institut Pasteur de Bangui were screened for ESBLs. Antimicrobial drug susceptibility was determined by using the disk diffusion method (Bio-Rad, Marnes la Coquette, France) on Mueller-Hinton agar (MHA MHA
microangiopathic hemolytic anemia. ) and interpreted according to the recommendations of the Comite de l'Antibiogramme de la Societe Francaise de Microbiologie (CASFM) (www.sfm.asso.fr). ESBL-producing Enterobacteriaceae were selected by the following criteria: susceptibility to cefoxitin; decreased susceptibility to cefotaxime (30 [micro]g), ceftazidime (30 [micro]g), or cefepime (30 [micro]g) (zone diameter <21 mm); and enhanced susceptibility in the presence of clavulanic acid by the double disk synergy test (3). For suspected ESBLs, the MICs of broad-spectrum cephalosporins were determined by using the agar dilution method.
We screened 450 Enterobacteriaceae for ESBLs during the study. We isolated and identified 17 (4%) ESBL-producing strains (Table). These strains were associated with urinary tract infection urinary tract infection (UTI),
n infection in one or more of the structures that make up the urinary system. Occurs more often in women and is most commonly caused by bacteria. , pneumonia in an AIDS patient, wound infection, vaginal or intestinal colonization, and ear infection. We found that 11 isolates were more resistant to cefotaxime (MIC [greater than or equal to] 256 [micro]g/mL) than to ceftazidime (MIC [less than or equal to] 128 [micro]g/mL), which suggests CTX-M-type enzymes. Enterobacteriaceae strains that harbor ESBLs were frequently associated with resistance to aminoglycosides and ciprofloxacin (Table).
The conjugal transfer of the resistance determinants was carried out in trypticase soy (TS) broth with rifampin-resistant Escherichia coli J53-2 as the recipient. Mating broths were incubated at 37[degrees]C for 18 h. Transconjugants were selected on MHA plates containing rifampin rifampin (rĭfăm`pĭn), antibiotic used in the treatment of tuberculosis. It is also used to eliminate the meningococcus microorganism from carriers and to treat leprosy, or Hansen's disease. (250 [micro]g/mL) and cefotaxime (2.5 [micro]g/mL). If conjugal transfer failed, plasmid DNA was extracted from donors with the Qiagen Plasmid Mini Kit (Qiagen, Courtaboeuf, France); 20 [micro]L of E. coli DH10B cells were transformed with plasmid DNA by electroporation electroporation (i·lekˈ·trō·p·rāˑ·sh according to the manufacturer's instructions (Bio-Rad). Transformants were incubated for 1.5 h at 37[degrees]C in TS broth and then plated on MHA plates supplemented with 2.5 [micro]g/mL cefotaxime.
Plasmid-encoded [beta]-lactamase genes were detected on clinical isolates and their tranconjugants or transformants by polymerase chain reaction polymerase chain reaction (pŏl`ĭmərās') (PCR), laboratory process in which a particular DNA segment from a mixture of DNA chains is rapidly replicated, producing a large, readily analyzed sample of a piece of DNA; the process is with oligonucleotide primer sets specific for the [bla.sub.TEM], [bla.sub.SHV], and [bla.sub.CTX-M] genes (4). PCR PCR polymerase chain reaction.
polymerase chain reaction
Polymerase chain reaction (PCR) assays were performed on total DNA DNA: see nucleic acid.
or deoxyribonucleic acid
One of two types of nucleic acid (the other is RNA); a complex organic compound found in all living cells and many viruses. It is the chemical substance of genes. extracted by using the commercial Qiagen DNA Mini Kit. The 3 [beta]-lactamase genes were detected in different clinical isolates (Table). PCR results showed that the strains were harboring [greater than or equal to] 2 different types of [beta]-lactamases.
Plasmid-encoded [beta]-lactamase genes were characterized by direct DNA sequencing with PCR primers. The nucleotide sequences were analyzed by the BLASTN (nucleotide basic local alignment search tool) program. For ESBLs, the gene types (SHV-2a, SHV-12, CTX-M-15, and CTX-M-3) were identified from different Enterobacteriaceae (Table). Only 1 strain (Enterobacter aerogenes) harbored 2 different ESBLs (CTX-M-3 and SHV-12). We identified TEM- 1 and CTX-M 15 enzymes, which are the most prevalent [beta]-lactamases detected in our strains.
ESBL-producing Enterobacteriaceae have been previously described in South Africa (5), Kenya (6), Senegal (7), Cameroon (8), Tanzania (9), and Nigeria (10). As described in these countries, we found that CTX-M-15, SHV-2a, and SHV-12 were the most prevalent enzymes. CTX-M-15, the most recently described ESBL ESBL Extended Spectrum Beta Lactamase
ESBL East Staffordshire Badminton League (UK) type, is particularly common in Bangui and seems to be closely related to E. coli, as was previously observed in Tanzania (9). This finding is also the first report of CTXM-3 in sub-Saharan Africa.
Multidrug resistance profiles involving non-[beta]-lactam antimicrobial drugs coselected these ESBL-producing isolates. We suggest that the misuse of antimicrobial drugs in the Central African Republic and the migratory flux of regional populations could result in emergence and selection of these ESBL phenotypes in the community. We could not establish a relationship between the different strains isolated in hospitalized and ambulatory patients. Because of the implications for treating such infections, particularly in developing countries, the spread of ESBL-producing Enterobacteriaceae merits close surveillance in the Central African Republic.
This work was financed by grants from Institut Pasteur de Bangui, Faculte de Medecine Pierre et Marie Curie Curie (kürē`), family of French scientists.
Pierre Curie, 1859–1906, scientist, and his wife,
Marie Sklodowska Curie, 1867–1934, chemist and physicist, b. , Universite Pierre et Marie Curie (Paris VI), and the European Community, contract LSHM-CT 2003-503335.
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Thierry Frank, * Guillaume Arlet, ([dagger]) ([double dagger]) Valerie Gautier, ([dagger]) Antoine Talarmin, * and Raymond Bercion *
* Institut Pasteur de Bangui, Bangui, Central African Republic; ([dagger]) Universite Pierre et Marie Curie (Paris VI), Paris, France; and ([double dagger]) Hopital Tenon AP-HP AP-HP Assistance Publique - Hôpitaux de Paris , Paris, France
Address for correspondence: Guillaume Arlet, Service de Bacteriologie-Hygiene, Hopital Tenon, AP-HP, rue de la Chine chine
the animal's backline. , 75970 Paris CEDEX 20, France; email: guillaume.arlet@ tnn.ap-hop-paris.fr