Exposure of Rural and Urban Populations in KwaZulu Natal, South Africa, to Fumonisin [B.sub.1] in Maize.

We surveyed households in rural and urban areas of KwaZulu Natal, South Africa, to assess the exposure of the inhabitants to fumonisin [B.sub.1] ([FB.sub.1]), a mycotoxin produced by Fusarium verticillioides. In southern African regions maize, used as a staple food by the population, is prone to F. verticillioides infection. Furthermore, high levels of [FB.sub.1] in maize have been associated with esophageal cancer in South Africa. We assessed exposure of the population to [FB.sub.1] at three levels, namely, by analyzing stored maize, plate-ready food, and feces. The positions of participating households in the rural area were recorded using geographic information systems (GIS) for ease and accuracy of follow-up. Of the 50 rural maize samples examined, 32% had levels of [FB.sub.1] ranging from 0.1-22.2 mg/kg, whereas 29% of the 28 cooked maize (phutu) samples contained [FB.sub.1] ranging from 0.1-0.4 mg/kg. The incidence and levels of [FB.sub.1] in feces were 33% and 0.5-39.0 mg/kg, respectively. Of the 49 urban maize samples analyzed 6.1% had a range of 0.2-0.5 mg/kg [FB.sub.1], whereas 3 of 44 fecal samples (6%) ranged between 0.6 and 16.2 mg/kg. No [FB.sub.1] was detected in urban phutu samples. Because these levels are lower than those published from regions in South Africa with high incidence of esophageal cancer, it may be concluded that the risk of esophageal cancer from [FB.sub.1] exposure is lower in the KwaZulu Natal region. Key words: esophageal cancer, fumonisin [B.sub.1], Fusarium verticillioides, high performance liquid chromatography (HPLC), maize. Environ Health Perspect 109:253-256 (2001). [Online 1 March 2001]

http://ehpnet1.niehs.nih.gov/docs/2001/ 109p253-256chelule/abstract.html

Certain mycotoxins have been associated with disease conditions among rural populations around the world, for example, aflatoxin [B.sub.1] and liver cancer (1). More recently, fumonisin [B.sub.1] ([FB.sub.1]) has been associated with the etiology of esophageal cancer in South Africa (2), and this has been supported by immunolocalization of [FB.sub.1] in esophageal cancer tissue (3). Fusarium verticillioides (mating type A), a producer of [FB.sub.1], has been identified as a major fungal contaminant on maize, especially in the homegrown crops intended for human consumption (4). The degree of maize infection is highly dependent on environmental factors, such as average temperatures below 22 [degrees] C and frequent rain during the first 2 weeks after silking, which increase the risk of colonization by Gibberella fujikuroi.

Because maize is the staple diet of the South African rural population, there is an increased risk of consumption of fumonisins. Studies carried out in four districts of Transkei, South Africa, linked high esophageal cancer rates in Butterworth and Kentani to the consumption of maize contaminated with elevated levels of [FB.sub.1] (117.3 mg/kg in 1989) as compared to lower levels in the control areas (Bizana and Lusikisiki; 11.3 mg/kg) (5). F. verticillioides was found to be the most dominant fungus infecting commercially produced maize in South Africa (6). In this case, the urban population may not be spared from [FB.sub.1] exposure.

We wanted to know whether other rural and urban populations of KwaZulu Natal, South Africa, are exposed to [FB.sub.1] at the same level as in the Transkei. Our study was carried out in Durban Metro and in villages of Mphise and Ngcolosi, a rural area near Kranskop, KwaZulu Natal, to determine the level of [FB.sub.1] in raw (stored) and cooked food. The analysis of fecal samples was also carried out as a short-term marker for exposure to [FB.sub.1] (7).

The use of geographic information systems (GIS) in health has been applied to epidemiologic investigations in South Africa, including malaria (8,9). In this study, we used GIS to locate and plot the rural dwellings for identification and follow-up sampling.

Materials and Methods

Chemicals. All chemicals, unless otherwise specified, were of Analar Grade. SPE SAX and [C.sub.18] cartridges containing 500 mg sorbent (10 mL capacity, Varian Bond-Elut) were purchased from Analytichem (Harbour City, CA, USA). For the mobile phase, we used methanol:0.1 M sodium dihydrogen phosphate (80:20, v/v) adjusted to pH 3.4 with orthophosphoric acid. o-Phthaldialdehyde (OPA) reagent was prepared by dissolving 40 mg OPA in 1 mL methanol and diluting with 5 mL 0.1 M sodium tetraborate and mercapthoethanol (50 [micro]L). Solvents used included acetonitrile/water (1:1, v/v), butanol, acetic acid, and methanol, all obtained from BDH Chemicals, Poole, England. o-Phosphoric acid (concentration [is greater than] 85%) was obtained from BDH Chemicals, Poole, England. We obtained the [FB.sub.1] standard (10 mg in sealed glass vials) from PROMEC, Cape Town, South Africa. The [FB.sub.1] standard was dissolved in acetonitrile/ water (1:1v/v) to give 1 mg/mL and 50 [micro]g/mL [FB.sub.1] working solutions.

Ethical approval We obtained ethical permission for the study from the Ethics Committee of the University of Natal (Nelson R. Mandela Medical School, H194/97). We also obtained informed consent from the clients before they were recruited into the study.

Collection of samples. We identified two villages in Tugela Valley (Mphise and Ngcolosi) for inclusion in the study. Recruitment of families into the study started by systematic random sampling (choosing every fifth of those who volunteered to take part in the study). We visited the recruited volunteers in their households and collected samples of processed foods [cooked, milled maize (phutu), fermented maize meal (amahewu), and local homebrew (isizulu, alcoholic beverage)] and unprocessed stored cereals. On the day after processed food was collected, feces samples were collected from the same households and stored at -20 [degrees] C pending analysis. We mapped the study area using GIS.

We carried out a similar study within the Durban metropolitan area, where households were visited (all subjects in the study were black). The households varied from formal to squatter (shack) settlements. Food and fecal samples were collected and analyzed as in the rural study. We included the Durban metropolitan area because of its urban character; people from this region consumed a more varied diet. Where maize is consumed in urban areas, it is subject to food safety regulation and is more likely to be mycotoxin-free.

Data analysis. We analyzed the questionnaire survey data using Excel (Microsoft, Redmond, WA, USA) and SPSS statistical packages (SPSS, Chicago, IL, USA).We used Mapinfo professional software (Mapinfo Corporation, Troy, NY, USA) to process geographic data and display household locations on maps.

Extraction and clean-up of [FB.sub.1] from cereal samples. Ground cereal (maize and sorghum) samples (100 g) were mixed thoroughly and a 25 g sample was extracted with 100 mL methanol/water (3:1 v/v) for 1 hr in a homogenizer (10). Liquid foods, isizulu and amahewu, were homogenized, and 50 mL of each was extracted with an equal volume of methanol/water (3:1 v/v). Phutu samples (25 g) were extracted using 100 mL 50% aqueous acetonitrile (adjusted to pH 2.7 using 0.1M HCl), as described above (11). The aqueous acetonitrile extracts were filtered, and 10 mL aliquots were carefully dried by rotary evaporation under low heat (60 [degrees] C). These samples were then reconstituted in 10 mL of methanol/water (3:1 v/v). The pH was adjusted to 6.5 and cleaned using strong anion exchange (SAX) solid phase cartridges, previously conditioned by washing with 5 mL methanol and 5 mL methanol/water (3:1 v/v). The columns were then washed with 5 mL methanol/water (3:1 v/v) and then 3 mL methanol, and [FB.sub.1] was eluted at the rate of 1 mL/min with 1% acetic acid in methanol (10 mL). The eluates were dried under a stream of nitrogen at 60 [degrees] C and stored at 4-8 [degrees] C until further analysis. The methanol/water extracts were filtered, the pH adjusted to 6.5, and 10 mL aliquots were applied to SAX.

Recovery and determination of [FB.sub.1] from cereal samples. Four samples of maize meal (25 g) containing no detectable [FB.sub.1] were placed in 250 mL conical flasks, thoroughly mixed with 10 mL methanol containing 100 [micro]g [FB.sub.1], and left to dry overnight. Two of the spiked samples were used to prepare phutu, a common traditional Zulu dish, by adding boiling water (20 mL) and a pinch of sodium chloride. The mixture was stirred to an even paste using a glass rod and heated to 93 [degrees] C with stirring. The temperature was then reduced to about 70 [degrees] C and the samples left to simmer for 15 min. After cooling, the cooked samples and the uncooked spiked samples were each extracted with 100 mL 50% aqueous acetonitrile, pH 2.7. The samples were continuously homogenized for 1 hr, filtered, and purified (10).

Analysis of [FB.sub.1] in fecal samples. Briefly, feces were lyophilized and then ground to a fine powder (7). A fraction (1.5 g) of the sample was extracted three times by vortexing for 1 min in a capped tube with 15 mL 0.1M EDTA, pH 5.2. The mixture was centrifuged at 2,000g for 10 min at 4 [degrees] C and the supernatant was removed; the extraction was repeated twice. The supernatants were combined, acidified to pH 2.9-3.2 with 5M HCl, and centrifuged at 4,000g for 10 min. A supernatant aliquot of 10 mL was applied to a Bond-Elut [C.sub.18] cartridge previously conditioned with 5 mL methanol and 5 mL water. The sorbent was first washed with 5 mL water, followed by 5 mL methanol/water (1:3, v/v) and finally with 3 mL methanol/water (1:1, v/v). Fumonisin [B.sub.1] was eluted with 15 mL methanol and the solvent was evaporated under a stream of nitrogen at 60 [degrees] C.

Quantitation by HPLC. We quantitated [FB.sub.1] by HPLC (10) using a Spectra Physics SCM400 system with a P2000 manual injector pump, a Nova-Pak 4 mm [C.sub.18] reversed phase analytical column (150 x 3.9 mm i.d.), and a Spectra FL2000 fluorescent detector (all from Waters, Milford, MA, USA). Detector excitation and emission wavelengths were set at 335 and 440 nm, respectively. The detection limit of the method was 50 ng/g [FB.sub.1] (signal/noise ratio of 5:1). The calibration curve was constructed to quantify up to 100 [micro]g/g ([R.sup.2] = 0.9983). Briefly, a sample (25 [micro]L) and [FB.sub.1] standard (50 [micro]g/mL) were pipetted into a tube, and 225 [micro]L of OPA was added and mixed. An aliquot of 20 [micro]L of derivatized sample or standard was injected into the column within 1 min of adding OPA. The mobile phase, methanol/sodium dihydrogen phosphate (80:20, v/v), was run isocratically at the rate of 1 mL/min. We identified [FB.sub.1] by its constant retention time and deduced its quantities by comparing the peak areas of the standards to those of the samples.

Results and Discussion

Analysis of food. To determine the amount of [FB.sub.1] being ingested by the subjects in this study, we analyzed plate-ready food as well as raw stored maize. We performed an experiment to assess the best possible way to recover [FB.sub.1] because we anticipated that preparation and cooking could affect [FB.sub.1] recovery levels. When maize meal was spiked with known amounts of [FB.sub.1] and cooked in a traditional way to produce phutu, we found that the pH of the material being extracted was critical. If the pH was not modified, only 36% of [FB.sub.1] was recoverable, whereas at pH 2.7 the recovery improved to 89% (Table 1). It is important to adjust this extract to pH 6.5 as soon as possible, to avoid [FB.sub.1] hydrolysis and for the subsequent cleanup. However, when [FB.sub.1]-spiked samples were cooked, extraction with unmodified solvent (50% aqueous acetonitrile) gave 35.9% recovery, whereas the modified extraction solvent (with pH 2.7) gave a recovery of over 85% [FB.sub.1] (Table 1).

Table 1. Results of [FB.sub.1] recoveries from 25 g of
cooked and uncooked maize-based food spiked
with a known amount (100 [micro]g) of [FB.sub.1].

                     [FB.sub.1]           Percent
Sample              recovered(c)          recovery

Phutu(a)     89 [micro]g ([+ or -] 3.4)      89
Phutu(b)     36 [micro]g ([+ or -] 2.9)      36
Maize meal   97 [micro]g ([+ or -] 1.3)      97

Recovery was performed after extraction with 50%
aqueous acetonitrile.

(a) Modified solvent, pH 2.7.

(b) Unmodified solvent pH.

(c) Recovery is a mean of two results.

Table 2 summarizes the results of food analysis for [FB.sub.1]. Of the 47 maize samples analyzed from the rural area, 15 (32%) contained [FB.sub.1] (0.1-22.2 mg/kg; average = 2.2 mg/kg), whereas 3 out of 49 (6%) of the maize samples from the urban area contained detectable levels (0.2-0.5 mg/kg; average = 0.3 mg/kg). The mean concentration of [FB.sub.1] in the rural maize was significantly different from that of the urban group (p = 0.014). This difference in [FB.sub.1] distribution is noteworthy because it shows that the rural populace is at a 6 times higher risk of [FB.sub.1]-associated disorders than the urban group (Fisher's exact test, [chi square] = 6.03). We observed that 29% of the cooked maize meal samples (phutu) from the rural area contained [FB.sub.1] at a range of 0.1-0.5 mg/kg (average = 0.3 mg/kg), whereas the urban samples had no detectable levels. This may reflect the [FB.sub.1] incidence in maize. As was shown in the recovery experiments, the preparation of phutu does not appreciably degrade [FB.sub.1]. The high end of the range of [is greater than] 22.2 mg/kg in stored maize is of some concern, but is not as high as the level found in regions of Transkei with a high incidence of esophageal cancer (117 mg/kg) (5).

Table 2. Analysis of cereals and feces for [FB.sub.1] from
the rural group and the urban control group using HPLC.

                          No. of
                        positives
Sample/      Samples       for             Range          Percent
region       analyzed   [FB.sub.1]        (mg/kg)        positives

Maize
  Rural(*)      47          15       0.1-22.2(*) (2.2)     31.9
  Urban(*)      49           3       0.2-0.5(*) (0.3)       6.1
Sorghum
  Rural         13          ND              NA              NA
  Urban         NS          NA              NA              NA
Amahewu
  Rural         14          ND              NA              NA
  Urban         NS          NA              NA              NA
Isizulu
  Rural         11          ND              NA              NA
  Urban         NA          NA              NA              NA
Phutu
  Rural         28           8       0.1-0.4 (0.3)         28.6
  Urban         39          ND              NA              NA
Feces
  Rural         40          13       0.5-39.0(*) (9.0)     32.5
  Urban         44           3       0.6-16.2(*) (6.8)      6

Abbreviations: NA, not analyzed; NS, not sampled; ND, not
detected (below the detection limit of 50 ng/g). Sample means
are shown in parentheses.

(*) The mean concentration of [FB.sub.1] was significantly
different (p = 0.014).

Fermented maize (amahewu), homebrew (isizulu), and unprocessed sorghum did not contain detectable levels of [FB.sub.1] from either region studied. Although G. fujikuroi (anamorph, F. moniliforme), mating type A, is the main contaminant of maize, sorghum often gets infected by members of mating type F, which are not producers of fumonisins (12). This could explain in part why we did not detect [FB.sub.1] in unprocessed sorghum. Although a limited amount of [FB.sub.1] is degraded during alcoholic fermentation of contaminated maize (13,14), isizulu (alcoholic fermented beverage) had no detectable levels of [FB.sub.1]. This is possible because isizulu is prepared using maize as a starter, but sorghum is the main component. Amahewu is made by boiling maize meal to soft porridge, which is then left to ferment overnight. This is a nonalcoholic (lactate) fermentation process that has been shown to degrade a number of mycotoxins (15). It is possible that [FB.sub.1], if it present in this product, could have also been degraded. From these results, it seems that the rural population should be advised to grow and use sorghum rather than maize. These communities can also be encouraged to consume traditional fermented products instead of unfermented maize-derived dishes, especially after our findings in this study have been confirmed.

Fecal analysis. Of the 40 rural fecal samples analyzed, 13 (33%) were positive for [FB.sub.1] (range = 0.5-39.0 mg/kg; average = 9.0 mg/kg) (Table 2), whereas only 3 out of 44 (7%) urban samples were positive (range = 0.6-16.2 mg/kg; average = 6.8 mg/kg). The mean concentration of [FB.sub.1] in the samples of the rural feces was significantly different to that from the urban area (p = 0.014). These results reflect the incidence of [FB.sub.1] in the maize that was consumed and is important in showing that analysis of feces is a useful short-term marker for [FB.sub.1] exposure, as the fecal samples were taken 24 hr after maize consumption. It is tempting to correlate the range of [FB.sub.1] (0.1-22.2 mg/kg) intake from maize in the rural population with the level found in feces samples (0.5-39.0 mg/kg), but clearly, other factors are also of importance here. These include personal habits, amount of food consumed, body weight, gut flora, and frequency of bowel movement. However, it does not seem unreasonable to assume, as a working rule, that in the rural population after 24 hr of ingestion a similar magnitude of [FB.sub.1] will be found in the dried feces. A time-course study of the period after the ingestion of contaminated maize is required to evaluate whether feces is a practical marker of [FB.sub.1] exposure. In general, Fisher's exact test showed that the rural population is 6 times more at risk ([chi square] = 6.03) of exposure than the urban population. This is possible because members of the rural population are routinely exposed to [FB.sub.1] from their diet (7).

GIS. In the case of the rural population, the various households were pinpointed using GIS and their locations are presented in Figure 1. This proved highly valuable because it avoided ambiguity and ensured that repeated visits and follow-up were easy, even by different workers in the field.

[ILLUSTRATION OMITTED]

Conclusions

This study has shown that the rural communities are at a much higher risk of exposure to [FB.sub.1] from consuming maize than the urban communities. However, this risk is minimized when nonalcoholic fermented maize such as amahewu is consumed. Degradation of [FB.sub.1] in this lactate fermentation process is not well understood and warrants further investigation. There is evidence that sorghum and its products are less prone to [FB.sub.1] contamination; furthermore, the drier areas such as Tugela Valley seem to favor its cultivation. It would be wise to encourage rural communities to grow and consume sorghum. It must, however, be noted that the sample size in this study was small. Another survey with a larger sample size, covering other areas of KwaZulu Natal, should be carried out to give a more comprehensive conclusion.

REFERENCES AND NOTES

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(11.) Murphy PA, Hendrich S, Hopmans EC, Hauck CC, Lu Z, Buseman G, Munkvold G. Effect of processing on fumonisin content of maize. In: Fumonisins in Food (Jackson LS, DeVries JW, Bullerman BL, eds). New York:Plenum Press, 1996;323-334.

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(13.) Bothast RJ, Bennett GA, Vancauwenberge JE, Richard JL. Fate of fumonisin [B.sub.1] in naturally contaminated maize during ethanol fermentation. Appl Environ Microbiol 58:233-236 (1992).

(14.) Scott PM, Kanhere SR, Lawrence GA, Daly EF, Farber JM. Fermentation of wort containing added ochratoxin A and fumonisin [B.sub.1]. Food Addit Contain 12:31-40 (1995).

(15.) Gqaleni N, Shandu NR, Sibiya P, Dutton MF. Indigenous non-alcoholic fermentations and mycotoxin degradation [Abstract]. In: International Symposium on Mycotoxins in the Food Chain (a Satellite Symposium of the IUTOX 8th International Congress of Toxicology), Toulose, France. (Le Bars J, Galtier P, eds). Reveue de Medecine Veterinaire 149:563 (1998).

Paul K. Chelule, Nceba Gqaleni, Michael F. Dutton, and Anil A. Chuturgoon

Environmental Health Research Unit, School of Medical Sciences, Nelson R. Mandela School of Medicine, University of Natal, Congella, South Africa

Address correspondence to M.F. Dutton, Environmental Health Research Unit, School of Medical Sciences, Nelson R. Mandela School of Medicine, University of Natal, Private Bag 7, Congella 4013, South Africa. Telephone: [phone]. Fax: + 27 031 260 4455. E-mail: [e-mail]

We thank the chiefs, the people of Tugela Valley, and S.E. Mncube for consenting to work with us.

This project was funded by the the National Research Fund-Franco and the Cancer Association of South Africa; P.K. Chelule received a scolarship from the Joint Financial Programme for Co-operation in Higher Education/Netherlands Organization for Internation Co-peration in Higher Education.

Received 20 March 2000; accepted 16 October 2000.