Effects of estradiol 17-beta and tamoxifen on MCF-7 human breast cancer cells.Concerns for human safety have been expressed as estrogens Estrogens Hormones produced by the ovaries, the female sex glands. Mentioned in: Acne, Polycystic Ovary Syndrome estrogens (es´trōjenz), n. are considered to be probable female carcinogens and have impact on reproductive system. We have examined in vitro effects of estradiol 17-beta, and tamoxifen on estrogen responsive MCF-7 human breast cancer cells. Estradiol 17-beta ([10.sup.-]6 and [10.sup.-4] M) and tamoxifen ([10.sup.-6] and [10.sup.-4] M) were added to the cultures of MCF-7 cells, and incubated for 24 hours Adv. 1. for 24 hours - without stopping; "she worked around the clock" around the clock, round the clock . Morphological changes were observed under phase-contrast microscope. The MTT assay was performed to assess cell viability. We have observed very low level of cell viability with [10.sup.-4] M tamoxifen when compared to the DMSO DMSO dimethyl sulfoxide. DMSO n. Dimethyl sulfoxide; a colorless hygroscopic liquid obtained from lignin, used as a penetrant to convey medications into the tissues. DMSO, n. control (P <0.05). There was a minor increase in cell viability with [10.sup.-6] M estradiol 17-beta. However, [10.sup.-4] M tamoxifen again significantly inhibited (P <0.01) the cell viability when compared to [10.sup.-6] M estradiol 17-beta. These results indicate that exposure of estrogen and tamoxifen to human breast cancer cells yielded changes in cell shape and cell viability. This may provide a useful model to study steroid hormone regulation of cytoskeleton cytoskeleton System of microscopic filaments or fibres, present in the cytoplasm of eukaryotic cells (see eukaryote), that organizes other cell components, maintains cell shape, and is responsible for cell locomotion and for movement of the organelles within it. in responsive human tumor cells. * Supported by grants from NIH "Not invented here." See digispeak. NIH - The United States National Institutes of Health. MD00233 and HD38342, and NSF HRD-0217602 |
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