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Drought stress and sowing date effects on yield and some grain traits of rapeseed cultivars.

Introduction

Oilseed rape(Brassica napus L.) is a member of the mustard (Brassicaceae) family and has become one of the most important sources of the vegetable oil in the world. Its oil also has potential in developing biodiesel market [5]. Also rapeseed is the single most important winter-spring oil crop that is globally recognized as an alternative to temperate cereals in the winter - spring grown season of the most temperate agricultural regions [16]. Water stress and temperature can reduce crop yield by affecting both source and sink for assimilates; also canola response to stress depends on the developmental stage, and seed yield in canola depends on the events occurring prior to and flowering stage [18]. Water saving and efficient use of precipitation are the priorities for agricultural improvement in arid and semiarid regions. Although limited regional and global research is available on oilseed rape production under irrigated conditions, it is known to be sensitive to water deficit conditions [1,24,20]. Gammelvind et al. [7] reported that water deficient in late vegetative and early reproductive growth stages reduces photosynthetic rate in leaves and yield. Johnston et al. (2002) resulted once the minimum water use of approximately 127 mm was achieved, seed yield of canola increased at the rate of 6.9-7.2 Kg [ha.sup.-1] [mm.sup.-1]. Water stress at flowering negatively influenced the formation of pod and seed size, resulting in lower final seed yield. Suttal et al. [21] have observed that seed yield was positively correlated to total precipitation and negatively correlated to mean maximum daily temperature that belonged to sowing date.

In the lower rainfall area, combination of the early sowing date with early flowering cultivar would be essential for the production of high seed yield [23]. Hocking and stapper [11] reported that, in Australia, seed yield of canola was reduced by 35% for a May sowing and by 67% for a July sowing, compared an April sowing. Stapper & Fischer [25] reported that late sowing date resulted in a shortening of the per-flowering period and decreases in seed yield, harvest index and yield component. The late sowing usually causes a decline in grown, leaf area, and a faster maturation [15].

Phospholipids (PL) have moderate antioxidant activity especially in the presence of phenolic antioxidants and/or acidic synergists [14]. PLs have therapeutic properties and used to improve human physiological and mental performance, lowering cholesterol levels, and treating neurological disorders [9]. Nevertheless, PLs in rapeseed oil are considered as undesirable impurities causing refining problems, oil losses due to the formation of emulsions during the alkali treatment [26]. Crude oil contains two type of PL: hydratable and non-hydratable phospholipids. Crude oil from rapeseed, which has been damaged in the field, during storage or handling and transportation, contain significant amount of non-hydratable phospholipids [27].

The importance of sowing date and drought stress have been reported in many researches [4,19,12], but little information is available on the interactive effects of delayed sowing date and drought stress on grain yield, oil percent, oil yield, protein percent, phosphor content and phospholipids of rapeseed. Therefore the main objective of these studies was to assess these traits of rapeseed cultivars in the Mediterranean types regions in delayed sowing date and interruption of irrigation at reproductive stage.

Material And Methods

Drought stress and sowing date effects on some physiological and agronomical traits of advanced Rapeseed (Brassica napus L.) cultivars were assessed during the 2008-2010 growing seasons at Karaj, Iran (50[degrees]75'E, 35[degrees]9'N; 1313 m a.s.l) with a three replicated randomized complete block design arranged in factorial split-plot. Treatments were included: Irrigation and sowing date as main plots, in this regard irrigation considered in two different levels of normal irrigation (control) ([I.sub.1]), and interruption of irrigation at reproductive stage (drought stress) ([I.sub.2]) and sowing date considered in two different levels including second half-September ([D.sub.1]) and second half-October (delayed sowing date) ([D.sub.2]), and Cultivars as sub plots. RGS006 ([V.sub.1]), Hyola330 ([V.sub.2]), RG4403 ([V.sub.3]), RG405/02 ([V.sub.4]), Hyola401 ([V.sub.5]) were used as experimental cultivars.

Each experimental plot consisted of six rows, with four m long and 30 cm spaced between rows and five cm distance between plants on the rows. Two side rows considered as margin and four middle lines considered for determination of traits such as grain yield. Soil samples were taken in depth of 0-30 and 30-60 cm, and according to the soil tests data, P and K were applied at a rate of 75 kg [P.sub.2][O.sub.5] [ha.sub.-1] and 50 kg [K.sub.2]O [ha.sub.-1] pre-plant in the form of triple superphosphate and [K.sub.2]S[O.sub.4], respectively and incorporated in the soil. S was applied at a rate of 75 Kg [ha.sup.-1] in the form of urea in three stages: one-third in 2-4 leaves stage, one-third in stemming stage and one-third in flowering stage. The soil was a deep silt-loam with 6.8 (1:1 [H.sub.2]O) pH, 0.76 dS/m EC, 1.3% organic mater, and bulk density of 1.6 g [cm.sup.-3]. Water content at field capacity and permanent wilting point was 27.5 and 14%, respectively. Weather data (precipitation, maximum and minimum of temperature and relative humidity) were recorded and reported as mean monthly data for the 2 years that the studies were conducted (Table1).

The following traits were assessed: grain yield, oil percent, oil yield, protein percent, phosphor content, phospholipids. Grains were dried at 40[degrees]C for four hours under vacuum condition to less than 5% moisture content and then milled to desired particle size by a mortar. Oil was extracted by AOCS method [2]. Oil content of the samples was expressed on percent based on whole seed. Phosphor content was extracted by IUPAC standard with IID.16, 20. Number; by preparing of ash solution in acid and determine of absorption amount with spectrophotometer in [729.sup.nm] and then compare with standard sample [22].

Results analyzed by MSTAT-C software and Duncan's multiple rang test at %5 level used for mean comparison.

Result:

Combined analysis of variance for some rapeseed traits are presented in table 2. In all assessed traits, first sowing date ([D.sub.1]) and normal irrigation ([I.sub.1]) were better than delay sowing date ([D.sub.2)] and drought stress ([I.sub.2]) conditions. Simple effects study of sowing date and irrigation showed that rapeseed was more sensitive to delay sowing date in comparison with drought stress, generally. As an example, oil yield at [D.sub.2] and [I.sub.2] conditions was 559.22 and 805.66 kg [ha.sup.-1] respectively.

Study of interaction effects of sowing date and irrigation revealed that [D.sub.1][I.sub.2] was superior to [D.sub.1][I.sub.2] conditions as we see for example grain yield under [D.sub.1][I.sub.2] by average of 2885 kg [ha.sup.-1] in comparison with grain yield under [D.sub.1][I.sub.2] by average of 1729 kg [ha.sup.-1].

Study on simple cultivar affect showed that the best cultivar regarding yield (3228 kg [ha.sup.-1]), oil percent (42.54%), oil yield (1339 kg [ha.sup.-1]), protein percent (34.96%), grain phosphor content (921.1 ppm) and phospholipids content (27630 ppm) were: [V.sub.2], [V.sub.1], [V.sub.1], [V.sub.2], [V.sub.5] and [V.sub.5], respectively.

Interaction effects of sowing date and cultivars (DV) showed that in delay sowing date ([D.sub.2]), [V.sub.2] had the most oil percent (39.93%), oil yield (625.7 kg [ha.sup.-1]), phosphor content (662.1 ppm), and phospholipids (19863 ppm); also, [V.sub.3] and [V.sub.5] with 1603 kg [ha.sup.-1] grain yield and 26.25% protein percent respectively, were the best cultivars in this condition that recommended (Fig 1- 6).

But multiple analysis of variance for irrigation and cultivars interaction effects (IV) showed that: in the drought stress condition from reproductive stage, [V.sub.1] with the most grain yield (2129 kg [ha.sup.-1]), oil yield (874.2 kg [ha.sup.-1]), protein percent (28.10%); and also [V.sub.2] with most production of oil percent (40.19%), phosphorcontent (630 ppm) and phospholipids (18900 ppm) were the best cultivars in this condition that recommended (fig 1-6).

Therefore, for any reason such as delayed harvesting of one spring crop, compelled to fall delayed planting to obtain maximum oil yield [V.sub.2]; and in drought stress condition from reproductive stage or deficit irrigation, [V.sub.1] recommended.

General results and interaction effects of sowing dates, irrigation regimes and cultivars showed in table 3.

According to table 3, superior cultivars for each trait and condition brought that recommended for the same conditions. For example, if maximum protein percent under delayed-sowing with normal irrigation was the main goal of production, [V.sub.2] would be suggested. Also, rapeseed cultivation not recommended under [D.sub.2][I.sub.2] due to low production in this condition in all cultivars.

[FIGURE 1 OMITTED]

[FIGURE 2 OMITTED]

[FIGURE 3 OMITTED]

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[FIGURE 5 OMITTED]

[FIGURE 6 OMITTED]

Discussion:

In a developing country with arid and semiarid climate such as Iran and due to its growing population, increasing food requirement and limited water resources, deficit irrigation merits consideration.

Irrigation scheduling is particularly important since many field crops are more sensitive to water deficit at a specific phonological stage. For example, water sensitive stages are flowering and boll formation stages in cotton, vegetative growth stage in soybean, flowering and grain filling stages in wheat and vegetative forming stages of sunflower and sugar beet [13]. Oilseed rape yield data in different locations under rain-fed and irrigation conditions are also available. For instance: in the different regional of Australia [6,10,13], and in the south west region of Indian Punjab [3], In Iran [8] found yield to be in the range of 1.0-5.3 t [ha.sup.-1].

Numerous research studies for different climates have shown that sowing date influences the growth, seed yield and quality of rapeseed [11,16,28]

Conclusion:

This study provides new information showed that oilseed rape significantly sensitive to delayed sowing date and also, interrupting irrigation at reproductive stage, causing to yield decrease significantly.

Reference

[1.] Al-Tabet, S.S., 2003. Growth and yield of oilseed rape in response to water salinity. The Journal of Agricultural Science Mansoura University, 28(2): 761-762.

[2.] AOCS., 1993. official methods and recommended particles of the American oil chemists society. AOCS press. Champaign. U.S.A.

[3.] Buttar, G.S., H.S. Thind, & M.S. Aujla, 2006. Method of planting and irrigation at various levels of nitrogen affect the seed yield and water use efficiency in transplanting oilseed rape (Brassica napus L.). Agricultural Water Management, 85: 253-260.

[4.] Chango, G., P.B.E. Mc Vetty, 2000. Relationship of physiological characters to yield parameters in oilseed rape (B. napus). Can. J. Plant Sci., 81: 1-6.

[5.] Economic research service USDA., 1996. Crambe, industrial rapeseed and tung provide valuable oils. Industrial Used of Agricultural Materials, 9: 17-23.

[6.] Farre, I., M. Robertson, & S. Asseng, 2007. Reliability of oilseed rape production in different rainfall zones of western. Australian Journal of Agricultural Research, 58(4): 326- 334.

[7.] Gammelvind, L.H., J.K. Schjoerring, V.O. Mogensen, C.R. Jenson, J.G.H. Bock, 1996. Photosynthesis in leaves and siliques of winter oilseed rape (Brassica napus L.) Plant Soil., 186: 227-236.

[8.] Hamzei, J., A.D.M. Nasab, F.R. Khoei, A. Havanshir and M. Moghaddam, 2007. Critical period of the weed control in three winter oilseed rape (Brassica napus L.) cultivars. Turkish Journal of Agricultural and Forestry, 31: 83-90.

[9.] Hidalgo, F.J., R. Zamora, 2006. Peptides and proteins in edible oils: stability, allergenicity and new processing trends. Trends in food science & Technology, 17(2): 56-63.

[10.] Hirth, J.R., P.J. Haines, A.M. Ridley and K.F. Wilson, 2001. Lucerne in crop rotation on the Riverine Plains 2. Biomass and grain yield, water use efficiency, soil nitrogen and profitability. Australian Journal of Agricultural Research, 52: 279-293.

[11.] Hocking, P.J., M. Stapper, 2001. Effect of sowing time and nitrogen fertilizer on canola and wheat, and nitrogen fertilizer on Indian mustard. I. dry maters production, grain yield and yield component. Aust. J. Agric. Res., 52: 623-634.

[12.] Johnston, A.M., D.L. Tanaka, P.R. Miller, S.A. Brandt, D.C. Nielsen, G.P. Lafond, N.R. Riveland, 2002. Oilseed crop for semiarid croping systems in the northern Great Plains. Agron. J. 94: 231-240.

[13.] Kirda, C., 2002. Deficit irrigation scheduling based on plant growth stages showing water stress tolerance. Deficit irrigation practices. In: FAO corporate document repository, 22: 3-10. Rome.

[14.] Kourimska, L., J. Pokorny, Z. Reblova, 1994. Phospholipids as inhibitors of oxidation during food storage and frying. PrehrambenotehnolBiotehnol. Rev., 32(2-3): 9194.

[15.] Mckay, K.R., A.A. Schneiter, 1990. Response of spring canola and Crambe to several dates of planting (In 1990 agronomy abstracts). ASA. Madison WI.

[16.] Miralles, D.J., B.C. Ferro, G.A. Slafer, 2001. Developmental responses to sowing data in wheat, barley and rapeseed. Field Crops Research, 71: 211-223.

[17.] Mendham, N.J., P.A. Salsbury, 1995. Physiology, crop development, growth and yield. In: kimber, D.S., Macgregor, D.I. (Eds.), Brassica oilseed: production and utilization. CAB international, London, pp:11-64

[18.] Morrison, M.J., D.W. Stewart, 2002. Heat stress during flowering in summer Brassica . Crop Sci., 42: 794-803.

[19.] Nielsen, D.C., 1997. Water use and oil of rape under dryland condition in the center Great Plains. Journal of Production Agriculture., 10(2): 307-313.

[20.] Nuttal, W.F., A.P. Moulin, L.J. Townley-smith, 1992. Yield response of canola to nitrogen, phosphorous, precipitation and temperature. Agron. J. 84: 765-768.

[21.] Paquat, C., 1970. international union of pure and applied chemistry, standard methods for the analysis of oils, fats and derivatives. (6th edn). Pergamon press. U.K.

[22.] Si, P., G.H. Walton, 2004. Determination of oil concentration and seed yield in canola and Indian mustard in the lower rainfall areas of western Australia. Aust. J. agric. Res., 55: 367-377.

[23.] Sinaki, J.M., E.M. Heravan, A.H.S. Rad, G. Noormohamadigand, & G. Zarei, 2007. The effect of water deficit during growth stage of oilseed yield (Brassica napus L.). American Eurasian Journal of Agricultural environmental science, 2(4): 417-422.

[24.] Stapper, M., R.A. Fischer, 1990. Sowing date and planting spacing influence on high-yielding irrigated wheat in southern New south Wales. I. Phasic development, canopy growth and spike production. Aust. J. Agric. Res., 41: 997-1019.

[25.] Szydlowska-Czerniak, A., 2007. MIR spectroscopy and partial least-squares regression for determination of phospholipids in rapeseed oils at various stages of technological process. Food chemistry, (105): 1179-1187.

[26.] Szydlowska-Czerniak, A., E. Szlyk, 2003. Spectrophotometric determination of total phosphorous in rapeseeds and oils at various stages of technological process: calculation of phospholipids and non-hydra table phospholipids contents in rapeseed oil. Food chemistry, (81): 613-619.

[27.] Taylor, A.J., C.J. Smith, 1992. Effect of sowing date and seeding rate on yield components of irrigated canola (Brassica napus L.) grown on a red-brown Earth in Southern-eastern Australia. Aus. J. Agric. Res., 43(7): 1629-1641.

(1) Babak Delkhosh, (2) Amir Hossein Shirani Rad, (3) Zahra Bitarafan, (4) Gelareh Mousavi nejad

(1) Department of Agro Ecology, Science and Research Branch, Islamic Azad University, Tehran, Iran

(2) Department of Oilseed Crops, Seed and Plant Improvement Institute, Karaj, Iran

(3) Department of Agronomy, Science and Research Branch, Islamic Azad University, Tehran, Iran

(4) Department of food science and industries, Tehran university, Tehran, Iran

Corresponding Author

Babak Delkhosh, Department of Agro Ecology, Science and Research Branch, Islamic Azad University, Tehran, Iran

E-mail: b.delkhosh@gmail.com, Tel. +989122894200
Table 1: precipitation, average monthly temperatures and relative
humidity in two years of study

Month 2008-2009

 [T.sub.min] [T.sub.max] Precipitation Min. Max.
 ([degrees]C) ([degrees]C) (mm) R.H. R.H.
 (%) (%)

Sep 16.23 31.1 0 15.46 64.57
Oct 10.93 24.29 4.7 25.7 65.2
Nov 5.66 18.2 33.2 26.3 64.7
Dec -0.9 7.7 56.8 44.9 81.3
Jen -9.83 -1.51 59.1 59.03 90.72
Feb -2.82 7.34 23.0 47.11 82.75
Mar 7.38 21.06 3.4 19.33 57.2
Apr 11.53 24.26 12.6 18.36 59.32
May 13.64 27.4 7.1 18.16 60.53
Jun 16.1 33.56 0.2 17.66 69.46

Month 2009-2010

 [T.sub.min] [T.sub.max] Precipitation Min. Max.
 ([degrees]C) ([degrees]C) (mm) R.H. R.H.
 (%) (%)

Sep 17.1 31.36 2.2 18.2 58.26
Oct 11.7 23.83 10 26.03 68.10
Nov 4.46 13.33 11.6 45.07 86.07
Dec -0.6 9.06 26.3 42.00 78.64
Jen -1.7 7.0 14.7 43.83 85.0
Feb 2.85 11.53 45.1 38.35 80.6
Mar 4.93 16.74 19.2 24.67 61.8
Apr 6.00 17.4 54.7 34.8 75.66
May 13.61 26.64 19.9 25.96 67.00
Jun 15.4 31.26 6.8 17.86 64.79

Table 2: analysis of variance for yield and some traits of rapeseed
oil in the combined

 d.f Grain Oil Oil
S.O. V yield percent yield

Year (Y) 1 n.s ** *
Error 4 - - -
Sowing date (D) 1 ** ** **
Y.D 1 * n.s *
Irrigation (I) 1 ** ** **
Y.I 1 * n.s *
D.I 1 ** * *
Y.D.I 1 * * *
Error 12 - - -
Cultivar (C) 4 ** ** **
Y.C 4 * * *
D.C 4 ** ** *
Y.D.C 4 * * *
I.C 4 * * *
Y.I.C 4 * * *
D.I.C 4 ** ** **
Y.D.I.C 4 * * *
Error 64 - - -
Total 119 - - -

 Protein Phosphor Phospholipids
S.O. V percent content

Year (Y) * n.s *
Error - - -
Sowing date (D) ** ** **
Y.D * * n.s
Irrigation (I) ** ** **
Y.I n.s * *
D.I ** ** *
Y.D.I n.s * *
Error - - -
Cultivar (C) ** ** **
Y.C * * *
D.C ** ** **
Y.D.C * * *
I.C ** ** *
Y.I.C * * *
D.I.C ** ** **
Y.D.I.C * * *
Error - - -
Total - - -

n.s: non significant, *, ** significant at 5, 1% level, respective

Table 3: superior cultivars at interaction effects of sowing date,
Irrigation and cultivars (DIV) under study.

 [D.sub.1][I.sub.1] [D.sub.1][I.sub.2]

Grain yield(kg [ha.sup.-1]) [V.sub.2] (3471) [V.sub.1] (3186)
Oil percent (%) [V.sub.1] (43.02) [V.sub.2] (40.85)
Oil yield(kg [ha.sup.-1]) [V.sub.2] (1451) [V.sub.1] (1340)
Protein percent (%) [V.sub.5] (37.12) [V.sub.2] (33.98)
Phosphor (ppm) [V.sub.5] (1120) [V.sub.2] (765.5)
Phospholipids(ppm) [V.sub.5] (33610) [V.sub.2] (22970)

 [D.sub.2][I.sub.1] [D.sub.2][I.sub.2]

Grain yield(kg [ha.sup.-1]) [V.sub.2] (2151) [V.sub.3] (1432)
Oil percent (%) [V.sub.2] (40.34) [V.sub.7] (39.52)
Oil yield(kg [ha.sup.-1]) [V.sub.2] (866.6) [V.sub.3] (541.0)
Protein percent (%) [V.sub.5] (29.82) [V.sub.5] (22.69)
Phosphor (ppm) [V.sub.2] (829.5) [V.sub.5] (500.8)
Phospholipids(ppm) [V.sub.2] (24940) [V.sub.5] (15024)
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Title Annotation:Original Article
Author:Delkhosh, Babak; Rad, Amir Hossein Shirani; Bitarafan, Zahra; nejad, Gelareh Mousavi
Publication:Advances in Environmental Biology
Article Type:Report
Geographic Code:7IRAN
Date:Jan 1, 2012
Words:3283
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