DNA amplification: current technologies and applications.
Horizon Bioscience, Norfolk, UK IBSN IBSN Infantile Bilateral Striatal Necrosis : 0-9545232-9-6 Pages: 335, Price: U.S.$180
DNA amplification is a powerful technique that has had an immense impact on scientific research in the past 2 decades. While polymerase chain reaction polymerase chain reaction (pŏl`ĭmərās') (PCR), laboratory process in which a particular DNA segment from a mixture of DNA chains is rapidly replicated, producing a large, readily analyzed sample of a piece of DNA; the process is (PCR PCR polymerase chain reaction.
polymerase chain reaction
Polymerase chain reaction (PCR) ) is still the most popular method, alternative methods of DNA amplification are constantly being developed. In addition, the extraordinary versatility of PCR has led to its use in novel ways that have opened new avenues of research. These novel methods for DNA amplification and the versatility of PCR are highlighted in DNA Amplification: Current Technologies and Applications.
The 17 chapters in this book are divided into 4 sections that focus on enzymes (3 chapters), thermal cycling methods (6 chapters), isothermal i·so·ther·mal
Of, relating to, or indicating equal or constant temperatures.
having the same temperature. methods (6 chapters), and the detection of non-DNA analytes by DNA amplification (2 chapters). Each chapter has a thorough description of methods and highly detailed protocols for applying the technique to at least 1 specific application. Several excellent chapters describe the uses of Phi29 DNA polymerase and of applications using isothermal rolling circle amplification. A chapter on multiple-displacement amplification details the isothermal amplification of total genomic DNA and should prove extremely useful for amplifying DNA DNA: see nucleic acid.
or deoxyribonucleic acid
One of two types of nucleic acid (the other is RNA); a complex organic compound found in all living cells and many viruses. It is the chemical substance of genes. in limited amounts, such as DNA from clinical samples. The final 2 chapters describe use of either real-time PCR or rolling circle amplification to detect and quantify non-DNA analytes, such as serum cytokines Cytokines
Chemicals made by the cells that act on other cells to stimulate or inhibit their function. Cytokines that stimulate growth are called "growth factors. , with much greater sensitivity than conventional enzyme-linked immunosorbent assay enzyme-linked immunosorbent assay
Enzyme-linked immunosorbent assay (ELISA)
A diagnostic blood test used to screen patients for AIDS or other viruses. methods.
This book is not for the novice scientist, as it does not describe basic DNA amplification fundamentals; rather, it is directed at those with a solid background in molecular biology who desire knowledge of cuttingedge applications. Although many of the detailed protocols will not be applicable to certain laboratory situations, the versatility of most of the methods described will allow them to be easily adapted to other studies. Therefore, this book will be a good addition to the library of researchers in molecular biology or to molecular diagnostics laboratories planning to expand their horizon beyond standard PCR amplification techniques.
Address for correspondence: Robert F. Massung, Centers for Disease Control and Prevention Centers for Disease Control and Prevention (CDC), agency of the U.S. Public Health Service since 1973, with headquarters in Atlanta; it was established in 1946 as the Communicable Disease Center. , 1600 Clifton Rd, Atlanta, GA 30333, USA; fax: 404-639-4436; email: firstname.lastname@example.org
Robert F. Massung, Centers for Disease Control and Prevention, Atlanta, Georgia, USA