Cutaneous melioidosis and necrotizing fasciitis caused by Burkholderia pseudomallei.

In areas where melioidosis is endemic, stress on the healthcare system is substantial. Because clinical manifestations are protean, the illness is difficult to diagnose, and cutaneous Burkholderia pseudomallei infections can progress to necrotizing fasciitis. While it is an uncommon complication of cutaneous melioidosis, necrotizing fasciitis is potentially fatal and requires aggressive management, including early diagnosis, appropriate antibiotics selection, and operative debridement.

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Melioidosis is a disease caused by a gram-negative bacterium, Burkholderia pseudomallei. Endemic to Southeast Asia, Taiwan, China, Central and South America, and northern Australia, sporadic infections occur throughout the world, usually in travelers to the disease-endemic areas (1-3). Humans are usually infected by soil contamination of puncture wounds. The respiratory tract is the most common site of infection (3). Cutaneous melioidosis with necrotizing fasciitis is rare: we found no reports of cutaneous melioidosis with necrotizing fasciitis and only one previous report of melioidosis with necrotizing fasciitis, which complicated postoperative drainage of a parotid abscess (4).

The Study

A 61-year-old Chinese man with a history of psoriasis and alcoholic liver cirrhosis sought treatment for left ankle swelling, erythema, and tenderness. He worked as a dishwasher in a restaurant and could not recall any antecedent trauma to the affected limb. He was febrile on admission and was empirically treated with intravenous penicillin and cloxacillin for cellulitis. Despite antimicrobial drug therapy, his lower limb infection progressed with formation of cutaneous blisters. A decision for wound debridement was made. During surgery, grayish necrotic fascia, extensive subcutaneous tissue necrosis, loss of resistance of the normally adherent superficial fascia to blunt dissection, and foul-smelling "dishwater" pus were noted. A diagnosis of necrotizing fasciitis was made.

The tissue specimen was spread onto blood agar plate (trypticase soy agar supplemented with 5% sheep blood, BBL [Becton, Dickinson and Company, Franklin Lakes, NJ]) and Ashdown's media, a selective media for B. pseudomallei. Characteristic colonial morphologic findings of B. pseudomallei were noted on blood agar and Ashdown's media. The organism's identity was confirmed with biochemical tests (positive oxidase reaction, simmons citrate) and microscopic morphologic findings. (The automated blood culture system used was the BACTEC Fluorescent Series blood culture system [Becton, Dickinson and Company].) Tissue (taken during surgery) and blood cultures grew B. pseudomallei susceptible to ceftazidime, amoxicillin/clavulanic acid, imipenem, chlorarnphenicol, and cotrimoxazole but resistant to ampicillin, amikacin, and gentamicin. Results of melioidosis serologic testing were also positive. The patient's chest roentgenogram was normal. A computed tomography scan of his abdomen and pelvis showed splenic abscesses, but no prostatic abscess was noted. A digital rectal examination demonstrated an enlarged prostate that was nontender. The patient underwent two further operations for wound debridement and split thickness skin grafting. He was treated with ceftazidime and doxycycline; when his fever persisted, his treatment was converted to intravenous imipenem. He responded well and was discharged after he completed a 4-week course of imipenem. On discharge, no oral antimicrobial drugs were dispensed.

One month later, the man was readmitted with fever associated with scrotal swelling and pain. On examination, a firm, tender right prostatic nodule was noted. A transrectal ultrasound showed abscesses in both prostatic lobes. The aspirate from the prostatic abscesses and blood cultures grew B. pseudomallei with antimicrobial drug susceptibilities identical to the organism cultured from his initial admission. A 6-week coarse of intravenous ceftazidime was given. He also underwent transurethral resection of the prostate; the histologic results indicated benign prostatic hyperplasia with acute on chronic inflammation. On discharge, he was prescribed oral amoxicillin-clavulanate (for 4 weeks) and doxycycline (for 1 year).

Seventeen months after cessation of oral antibiotics, he reappeared with a left temporal scalp abscess and lever. No organism was isolated from the scalp pus culture, but the blood culture again grew B. pseudomallei with identical antimicrobial drug susceptibilities. He was given 4 weeks of ceftazidime followed by amoxicillin-clavulanate (for 4 weeks) and doxycycline (long-term). After 8 months on oral doxycycline, the patient was symptom-free.

Conclusions

This patient history is representative of the protean clinical features of melioidosis. The disease has been called the great mimicker, with a wide range of clinical syndromes including pneumonia, visceral abscesses, soft tissue infections, septic arthritis, and septicemia (1,3,5). Lim et al. previously reported a case of melioidosis with a parotid abscess that was drained and subsequently progressed to necrotizing fasciitis (4). Although not previously reported, necrotizing fasciitis can be the initial sign of melioidosis. While cutaneous infections by B. pseudomallei are usually indolent soft tissue infections manifesting as cellulitis or abscesses (1-3,6), these soft tissue infections can progress to necrotizing fasciitis in melioidosis.

Melioidosis can remain latent for prolonged periods and reactivate with severe sepsis years after the initial infection (1,7). Melioidosis is characterized by relapses or recrudescence, especially in immunocompromised patients (1,7,8). For this patient, the first relapse occurred 1 month after the first hospital discharge. Another relapse occurred 17 months after cessation of oral maintenance antimicrobial drug therapy.

For patients with a known history of melioidosis, antimicrobial agents for B. pseudomallei should be used. B. pseudomallei are frequently intrinsically resistant to many antimicrobial agents, including aminoglycosides and first- or second-generation cephalosporins. Current recommended therapy of severe melioidosis includes intravenous ceftazidime or imipenem for 10 days to 4 weeks, followed by maintenance oral antimicrobial agents (amoxicillin-clavulanate or a combination of trimethoprim-sulfamethoxazole and doxycycline) for 10 to 18 weeks (9).

In the context of soft tissue infections, patients with a history of melioidosis developing cutaneous septic foci should be treated promptly and aggressively with antibiotics against B. pseudomallei in addition to the common organisms responsible for these infections such as Staphylococci aureus and streptococcal species. In this setting, an intravenous regime consisting of crystalline penicillin and cloxacillin may not be the appropriate initial antimicrobial drug regime (3). Awareness that soft tissue infection can progress to necrotizing fasciitis, a life--and limb-threatening condition, is important, and a high index of suspicion should be maintained. When necrotizing fasciitis is suspected, magnetic resonance imaging of the affected area should be performed to ensure early diagnosis, and aggressive debridement of all nonviable tissue should follow for an improved outcome (10).

Melioidosis is a major problem and a rampant disease in rural parts of Central and South America, North Australia, and Southeast Asia. In northeast Thailand, it is responsible for 20% to 40% of deaths from community-acquired septicemia (11). Its true incidence is probably underestimated due to under detection and limited availability of culture facilities (12). Awareness of the spectrum of soft tissue infections caused by B. pseudomallei, including the distinct possibility of necrotizing fasciitis at the extreme of the spectrum, is important. The successful management of necrotizing fasciitis requires appropriate antimicrobial drug selection and aggressive operative debridement.

Acknowledgment

We thank At-Ling Tan for the laboratory support she provided.

Dr. Wang is a trainee in internal medicine. She is currently working in the Department of Internal Medicine, Changi General Hospital, Singapore.

References

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(3.) Currie BJ, Fisher DA, Howard DM, Burrow JN, Lo D, Selva-Nayagam S, et al. Endemic melioidosis in tropical northern Australia: a 10-year prospective study and review of the literature. Clin Infect Dis 2000;31:981-6.

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(5.) Yee KC, Lee MK, Chua CT, Puthucheary SD. Melioidosis, the great mimicker: a report of 10 cases from Malaysia. J Trop Med Hyg 1988;91:249-54.

(6.) Tran D, Tan HH. Cutaneous melioidosis. Clin Exp Dermatol 2002;27:280-2.

(7.) Mays EE, Ricketts EA. Melioidosis: recrudescence associated with bronehogenic carcinoma twenty-six years following initial geographic exposure. Chest 1975;68:261-3.

(8.) Silbermann MH, Gyssens IC, Endtz HP, Kuijper EJ, van der Meer JTM. Two patients with recurrent melioidosis after prolonged antibiotic therapy. Scand J Infect Dis 1997;29:199-201.

(9.) Smith MD, Wuthiekanun V, Walsh AL, White NJ. In-vitro activity of carbapenem antibiotics against beta-lactam susceptible and resistant strains of Burkholderia pseudomallei. J Antimicrob Chemother 1996;37:611-5.

(10.) Wong CH, Chang HC, Pasupathy S, Khin LW, Tan JL, Low CO. Necrotizing fasciitis: clinical presentation, microbiology and determinants of mortality. J Bone Joint Surg Am 2003;85-A:1454-60.

(11.) Chaowagul W, White NJ, Dance DA, Wattanagoon Y, Naigowit P, Davis TM, et al. Melioidosis: a major cause of community-acquired septicemia in northeastern Thailand. J Infect Dis 1989;159:8909.

(12.) Parry CM, Wutheikanum V, Hao NT, Diep TS, Than LT, Loc PV, et al. Melioidosis in southern Vietnam: clinical surveillance and environmental sampling. Clin Infect Dis 1999;29:1323-6.

Address for correspondence: Chin-Ho Wong, Department of Plastic Surgery, Singapore General Hospital, Outram Road, Singapore 169608; fax: [phone]; email: [e-mail]

Yi-Shi Wang, * Chin-Ho Wong, [dagger] and Asok Kurup [dagger]

Changi General Hospital, Singapore [dagger] Singapore General Hospital, Singapore