Crimean-Congo hemorrhagic fever in Bulgaria.

We report the epidemiologic characteristics of Crimean-Congo hemorrhagic fever in Bulgaria, as well as the first genetic characterization of the virus strains circulating in the country in 2002 to 2003 that caused disease in humans.

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Crimean-Congo hemorrhagic fever virus (CCHFV) (genus Nairovirus, family Bunyaviridae) causes severe disease with a fatality rate as high as 30%. CCHFV is endemic in the Balkan Peninsula; a number of cases occur every year, sometimes in an epidemic form. Cases have been reported in Albania (1), Kosovo (2,3), and Bulgaria (4). Mountains approximately 1,500 m to 2,500 in high separate these countries from Greece, where no case of the disease has yet been identified. However, a CCHFV strain was isolated in Greece from Rhipicephalus bursa ticks, collected in May 1975 from goats of a flock in Vergina village, 80 km west of Thessaloniki (5). Antibodies against the virus were detected in the Greek human population (6). CCHFV is also endemic in Russia and in parts of Asia and Africa.

The virus is transmitted to humans by the bite of ixodid ticks (primarily of the Hyalomma genus) or by contact with blood or tissues from infected persons or infected livestock. The risk for spread of the virus from person to person is high, which occasionally results in nosocomial outbreaks. After an incubation period of 3 to 7 days, the patient has sudden onset of fever, chills, myalgia, and headache, which rapidly progress to severe illness; a hemorrhagic state follows with bleeding from the mucous membranes and petechiae, associated with thrombocytopenia and leukopenia (7).

CCHFV, like all members of the genus, is a negative-stranded RNA virus with a tripartite genome consisting of a small, medium, and large segment encoding the nucleocapsid protein; the glycoprotein precursor, which results in the two envelope glycoproteins G1 and G2; and the putative RNA-dependent polymerase, respectively (8).

We report (for the first time in English) the epidemiologic characteristics of the disease in Bulgaria. We also provide the first genetic characterization of the CCHFV strains circulating in the country from 2002 to 2003 that caused disease in humans.

Bulgaria is a country of 8 million inhabitants in the eastern part of the Balkans (Figure 1). CCHF was first recognized in the country in 1952 and became a reportable disease in 1953. In 1968, CCHFV was isolated from blood samples of two patients. Results from serologic investigations showed that approximately 20% of patients living in disease-endemic areas who reported a tick bite had antibodies to CCHFV (4). The seropositivity in animals in the disease-endemic areas can be as high as 50%. Most cases were reported from Plovdiv and Pazardgik (central Bulgaria), Haskovo and Kardgali (southeastern Bulgaria), Shumen (northeastern Bulgaria), and Burgass (eastern Bulgaria) (4). The most prevalent tick in Bulgaria is Ixodes ricinus; however CCHFV strains have been isolated from Hyalomma plumbeum (H. marginatum), Rhipicephalus sanguineus, and Boophilus calcaratus (9).

[FIGURE 1 OMITTED]

From 1953 to 1974, 1,105 CCHFV cases were reported to the Bulgarian Ministry of Health; the fatality rate was approximately 17%. Of them, 20 cases were nosocomial infections and 52% were fatal. In 1974, an immunization program was introduced for medical workers and military personnel in CCHF-endemic areas. The treatment regimen consisted of mouse brain preparation inactivated by chloroform, heated at 58[degrees]C, and adsorbed on Al[(OH).sub.3]. The first two doses were given at day 0 and day 30; a third dose was given 1 year later, and another dose was given 5 years after that (10). As a result, between 1975 and 1996, the number of reported CCHF cases was reduced to 279, with a fatality rate of 11.4%. No infection was reported from vaccinated military personnel (11).

Since 1997, a total of 124 cases occurred in Bulgaria, 27 of them fatal (Table 1). Most patients had been bitten by a tick; however, a few were infected through direct contact with CCHF patients. Only the eastern part of the country has been affected; two main foci exist, one in the southeast and a second one in northeast. The mean age of patients is 52 years (range 11-79 years). Most patients are men (74%), probably because they are mole frequently exposed to ticks bites during outdoor activities. The disease occurs mainly from March to July when ticks are more active. The main clinical symptoms are fever, malaise, nausea, epistaxis, petechiae, and bleeding from the gastrointestinal tract; the main laboratory findings are leukopenia, thrombocytopenia, and elevated transaminase levels.

To investigate the genetic relationships of the CCHFV strains circulating recently in Bulgaria, RNA was extracted from cell culture supernatant from six virus isolates. The virus had been isolated in a Vero E6 cell line from blood samples taken from CCHF patients who were infected in 2002 and 2003. The epidemiologic characteristics of the patients are shown at Table 2. A reverse-transcriptase-nested polymerase chain reaction (PCR) was applied to amplify a partial fragment of the S RNA genome segment by using two sets of primers, F2-R3 and F3-R2 (12). Purified PCR products were sequenced; the nucleotide sequences were submitted to the GenBank database and assigned the accession numbers AY550253-AY550258. After aligning the obtained Bulgarian CCHFV sequences with respective ones retrieved from GenBank, we constructed a phylogenetic tree with PHYLIP software (13) (Figure 2). All Bulgarian isolates were found to cluster together, with a genetic homology of 98.4% to 100% at the nucleotide level. Identical sequences were obtained from isolates originating from the same region in the same year.

[FIGURE 2 OMITTED]

The Bulgarian CCHFV strains were found to cluster with other Balkan strains from Kosovo and Albania, with a mean genetic difference of 2% and 1.2%, respectively. All Balkan strains clustered in the same branch with CCHFV strains from European Russia, such as STV/ HU29223 strain, isolated in 2000 from human blood in Stavropol (mean genetic difference 2.5%), and ROS/ TI28044, isolated in 2000 from Hyalomma marginatum ticks in Rostov (mean genetic difference 3.7%) (14). A "European CCHFV group," distinct from all others, is evident. An exception to the European group is the Greek strain AP92, isolated from R. bursa ticks (5), which forms an independent clade, which differs from the Bulgarian strains by 24%. This genetic difference is likely attributable to the different species of related ticks or to reassortment. Studies on the Greek strain are still in progress; they will help explain the genetic and pathogenic differences among this strain and respective strains from neighboring countries.

Although the genetic divergence among European strains is low, a great divergence is seen among European CCHFV strains and strains from other continents (Asia and Africa). As the number of CCHFV sequences derived from the S genome segment is growing, eight distinct clades can be seen: 1) strain AP92 from Greece; 2) strains from Senegal, Mauritania, and Iran; 3) strains from Senegal, Mauritania, and South Africa; 4) strains from Nigeria and Central African Republic; 5) strain from Uganda; 6) strains from Central Asia and China; 7) strains from Madagascar, Iran, and Pakistan; and 8) European strains (Russia, Albania, Kosovo, and Bulgaria) (Figure 2).

In conclusion, this report shows that the CCHV is endemic in Bulgaria and causes severe disease in the whole Balkan Peninsula (except Greece) and that the Bulgarian CCHFV strains are genetically similar to other Balkan virus strains (except AP92). CCHFV evolves relatively slowly, which suggests that the great genetic divergence among the strains is not time-dependent. Whether this divergence is because of the different tick species, the different geographic location, or any other reason, remains to be elucidated.

Table 1. Distribution of Crimean-Congo hemorrhagic fever cases and
related deaths in Bulgaria, 1997-2003

Y      No. of cases   No. of deaths (%)

1997        20             4 (20)
1998        15             3 (20)
1999         5             2 (40)
2000        10             1 (10)
2001        18             5 (28)
2002        56            12 (21)
2003        14             2 (14)

Table 2. Epidemiologic characteristics of patients whose blood
samples yielded Crimean-Congo hemorrhagic fever virus strains

No of isolate   Date of disease onset    Sex     Age (y)    Area

BUL1/03               June 2003          Male      60      Haskovo
BUL2/03               June 2003          Male      45      Shumen
BUL3/02              April 2002         Female     62      Shumen
BUL6/02               June 2002          Male      73      Plovdiv
BUL9/02               June 2002          Male      23      Shumen
BUL10/02             August 2002         Male      39      Haskovo

References

(1.) Papa A, Bino S, Llagami A, Brahimaj B, Papadimitriou E, Pavlidou V, et at. Crimean-Congo hemorrhagic fever in Albania, 2001. Eur J Clin Microbiol Infect Dis. 2002;21:603-6.

(2.) Drosten C, Minnak D, Emmerich P, Schmitz H, Reinicke T. Crimean-Congo hemorrhagic fever in Kosovo. J Clin Microbiol. 2002;40:1122-3.

(3.) Papa A, Bozovic B, Pavlidou V, Papadimitriou E, Pelemis M, Antoniadis A. Genetic detection and isolation of Crimean-Congo hemorrhagic fever virus, Kosovo, Yugoslavia. Emerg Infect Dis. 2002;8:852-4.

(4.) Vasilenko S, Chumakov M, Katzarov G, Mihailov A, Levi V, Kebedgiev G, et al. Investigations on Congo-Crimean hemorrhagic fever in Bulgaria 11. Serological examinations of people and animals in endemic and nonendemic for CCHF areas [article in Bulgarian]. Epidemiology, Microbiology, and Infectious Diseases. 1971;8:150-6.

(5.) Papadopoulos O, Koptopoulos G. Crimean-Congo hemorrhagic fever (CCHF) in Greece: isolation of the virus from Rhipicephalus bursa ticks and a preliminary serological survey. Zentbl Bakteriol Hyg Abt 1. 1980;Suppl 9; 189-93.

(6.) Antoinadis A, Casals J. Serological evidence of human infection with Congo-Crimean hemorrhagic fever virus in Greece. Am J Trop Med Hyg. 1982;31:1066-7.

(7.) Swanepoel R, Shepherd AJ, Leman PA, Shepherd SP, McGillivray GM, Erasmus MJ, et al. Epidemiologic and clinical features of Crimean-Congo hemorrhagic fever in southern Africa. Am J Trop Med Hyg. 1987;36:120-32.

(8.) Nichol ST. Bunyaviruses. In: Knipe DM, Howley PM, editors. Fields virology, vol. 2. Philadelphia: Lippincott Williams and Wilkins; 2001. p. 1603-33.

(9.) Vasilenko SM, Kirov I, Katzarov G, Chumakov M, Kebedjiev G, Butenko A, et al. Investigations on CCHF in Bulgaria [article in Bulgarian]. Proceedings of Hyg Epidemiol Inst. 1970;22:153-66.

(10.) Todorov S, Kovacheva T, Velcheva D, Katzaruv G. Congo-Crimean hemorrhagic fever--prophylaxis and treatment [article in Bulgarian]. Contemporary Medicine. 2001;42:54-60.

(11.) Kovacheva T, Velcheva D, Katzarov G. Studies on the morbidity of Congo-Crimean hemorrhagic fever before and after specific immunoprophylaxis [article in Bulgarian]. Infectology. 1997;34:34-5.

(12.) Rodriguez LL, Maupin GO, Ksiazek TG, Rollin PE, Khan AS, Schwarz TF, et al. Molecular investigation of a multisource outbreak of Crimean-Congo hemorrhagic fever in the United Arab Emirates. Am J Trop Med Hyg. 1997;57:512-8.

(13.) Felsenstein J. PHYLIP (Phylogeny Inference Package) version 3.5c. Seattle: Department of Genetics, University of Washington; 1993.

(14.) Yashina L, Petrova I, Seregin S, Vyshemirskii O, Lvov D, Aristova V, et al. Genetic variability of Crimean-Congo hemorrhagic fever virus in Russia and Central Asia. J Gen Virol. 2003;84:1199-206.

Dr. Papa is assistant professor in the First Department of Microbiology, School of Medicine of Aristotle University, Thessaloniki, Greece. Her major interests include the molecular biology and epidemiology of bunyaviruses and retroviruses.

Address for correspondence: Anna Papa, First Department of Microbiology, School of Medicine, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece; fax: [phone]; email: annap@ med.auth.gr

Anna Papa, * Iva Christova, ([dagger]) Evangelia Papadimitriou, * and Antonis Antoniadis *

* WHO Collaborating Center for Reference and Research on Arboviruses and Haemorrhagic Fever Viruses at Aristotle University of Thessaloniki, Thessaloniki, Greece; and ([dagger]) National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria