Construction of human myelin oligodendrocyte glycoprotein isoform 16. 3a. (Withdrawn).
Myelin/oligodendrocyte glycoprotein (MOG v. t. 1. To move away; to go off.
[
imp. & p. p. os> Mogged
r>;
p. pr. & vb. n. os> Mogging.] ) is an integral membrane
protein An Integral Membrane Protein (IMP) is a protein molecule (or assembly of proteins) that is permanently attached to the biological membrane. Such proteins can be separated from the biological membranes only using detergents, nonpolar solvents, or sometimes denaturing agents. found specifically in the central nervous system (CNS). MOG may
be a primary target antigen in immune-mediated demyelinating disorders
such as multiple sclerosis. In order to examine the role of MOG in the
CNS, the human MOG gene was spliced using reverse transcriptase
polymerase chain reaction polymerase chain reaction (pŏl`ĭmərās') (PCR), laboratory process in which a particular DNA segment from a mixture of DNA chains is rapidly replicated, producing a large, readily analyzed sample of a piece of DNA; the process is (RT-PCR RT-PCR
reverse transcriptase-polymerase chain reaction. See PCR1. ). This revealed MOG specific variants
that encoded different exons. Exon 3 encoded a hydrophilic domain
containing multiple stop codons that would result in truncation of MOG
prior to translation of its transmembrane domain. We constructed an
isoform, 16. 3a, which encoded this exon. The construction technique
used was splicing by overlap extension (SOE), which involves generating
the two fragments of interest in separate polymerase chain reactions
(PCR).
Kerry-Ann Stewart, Rush Oliver
Department of Biological and Physical Sciences
Benedict College
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