Construct food-grade cloning vectors for lactic acid bacteria.As you know, lactic acid bacteria The Lactic Acid Bacteria (LAB) comprise a clade of Gram positive, low-GC, acid tolerant, non-sporulating, non-respiring rod or cocci that are associated by their common metabolic and physiological characteristics. (LAB) are a commercially significant group of microorganisms. Genetically improving these bacteria requires the development of techniques and vectors for introducing or altering desired genes. The goals of research at the Cooperative Research Center for Food Industry Innovation (CRC (Cyclical Redundancy Checking) An error checking technique used to ensure the accuracy of transmitting digital data. The transmitted messages are divided into predetermined lengths which, used as dividends, are divided by a fixed divisor. , University of New South Wales The University of New South Wales, also known as UNSW or colloquially as New South, is a university situated in Kensington, a suburb in Sydney, New South Wales, Australia. , Biotechnology Department, Sydney NSW NSW New South Wales Noun 1. NSW - the agency that provides units to conduct unconventional and counter-guerilla warfare Naval Special Warfare 2052, Australia) are to construct a variety of food-grade cloning vectors for use in LAB and to improve the bacteriophage resistance of dairy starter cultures using genetic techniques. Investigators have improved their understanding of LAB genetics. They have identified and characterized the regulatory genes in the lactococcal operon that encodes copper resistance. They've also identified antisense RNA antisense RNA an RNA sequence which is complementary to a functional RNA. that helps control plasmid replication in lactococci. Application of the knowledge generated from this fundamental genetic research is being implemented toward the construction of more sophisticated food-grade cloning vectors. Two additional food-grade vectors have been constructed, providing a greater selection of vectors available for use. Some of these vectors have been successfully introduced into commercial starter strains and are highly stable. Several Streptococcus thermophilus Streptococcus thermophilus (official name Streptococcus salivarius subsp. thermophilus) is a Gram-positive facultative anaerobe. It is a cytochrome-, oxidase- and catalase-negative organism that is nonmotile, non-spore forming and homofermentative. shuttle vectors have been constructed, and genetic techniques have been established for this LAB species. Meanwhile, research into bacteriophage resistance has focused on consolidating data on phage phage: see bacteriophage. phage - A program that modifies other programs or databases in unauthorised ways; especially one that propagates a virus or Trojan horse. See also worm, mockingbird. The analogy, of course, is with phage viruses in biology. resistance mechanisms that have been isolated. Complete DNA sequences have been achieved for four abortive abortive /abor·tive/ (ah-bor´tiv) 1. incompletely developed. 2. abortifacient (1). 3. cutting short the course of a disease. a·bor·tive adj. 1. infection mechanisms and two restriction modification systems isolated from lactococci. Four other subcloned mechanisms are being characterized, providing for a good selection of mechanisms that can be used to construct multiple phage resistance units. Meanwhile, phage resistance mechanisms have been introduced into a food-grade vector, and these constructs are being characterized. Current efforts involve introducing the food-grade vectors carrying multiple phage resistance mechanisms into selected L. lactis and L. lactis subsp. cremoris strains. These constructs will be characterized to assess the effectiveness and stability of the phage resistance units in different hosts. Investigators plan to construct more versatile food-grade cloning vectors. Further information. Melissa Harvey; phone: +61 2 9385 1014; fax: +61 2 9385 1015; email: foodcrc@unsw.edu.au. |
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