Condition, reproductive activity, and gross biochemical composition of the Manila clam, Tapes philippinarum in natural and newly created sandy habitats of the southern coast of Korea.ABSTRACT Sand was added to the mudflat Mudflats are coastal wetlands that form when mud is deposited by the tides or rivers, sea and oceans. They are found in sheltered areas such as bays, bayous, lagoons, and estuaries. in a small bay on the southern coast of Korea in an attempt to create a new habitat for the Manila clam (Tapes philippinarum) in the muddy intertidal zone The intertidal zone, also known as the littoral zone, in marine aquatic environments is the area of the foreshore and seabed that is exposed to the air at low tide and submerged at high tide, i.e., the area between tide marks. . To evaluate whether the newly created sandy habitat was functionally similar to natural ones, seasonal variations in condition, reproductive activity, and biochemical composition of clams in created and natural conditions were compared from May 2000 to October 2001. Clams reared in the newly created and natural habitats had similar patterns and levels with respect to condition and tissue dry weight. Standardized animal condition and tissue dry weight of clams peaked in spring, when protein and carbohydrate reserves were at maximum levels, and declined progressively throughout the summer-autumn period to October, as a result of continuous spawning. Condition and tissue weight were quickly restored during the winter-spring period, concurrently with accumulation of protein and carbohydrate reserves. Similar biochemical compositions and reproductive cycles reproductive cycle
The cycle of physiological changes that begins with conception and extends through gestation and parturition. for the clam stocks in the two habitats are likely to be related to their similar environmental conditions, in particular food availability. Comparison of the isotopic signatures of T. philippinarum tissues and potential food resources suggested that food availability in the study area was mostly dependent on resuspension Noun 1. resuspension - a renewed suspension of insoluble particles after they have been precipitated
suspension - a mixture in which fine particles are suspended in a fluid where they are supported by buoyancy of microphytobenthos, along with seasonal dynamics of phytoplankton phytoplankton
Flora of freely floating, often minute organisms that drift with water currents. Like land vegetation, phytoplankton uses carbon dioxide, releases oxygen, and converts minerals to a form animals can use. . These observations clearly show that newly created sandy habitats may provide habitat functions that enable Manila clams to have similar biological cycles to those in natural habitats.
KEY WORDS: Clam culture, Tapes philippinarum, condition, reproductive cycle, biochemical composition, created habitat
The Manila clam, Tapes philippinarum (Adams & Reeve 1850), is a mollusc mollusc
members of the phylum Mollusca, which comprises about 50,000 species. Includes snails, slugs and the aquatic molluscs—oysters, mussels, clams, cockles, arkshells, scallop, abalone, cuttlefish, squid. species that lives in sand, sandy-silt, or muddy-gravel sediments from the intertidal in·ter·tid·al
Of or being the region between the high tide mark and the low tide mark.
in to subtidal zones a few meters in depth. It is also one of the most widely exploited bivalve bivalve, aquatic mollusk of the class Pelecypoda ("hatchet-foot") or Bivalvia, with a laterally compressed body and a shell consisting of two valves, or movable pieces, hinged by an elastic ligament. resources for human consumption. Because of its high productivity and commercial importance, it is now cultivated worldwide. Numerous studies, particularly for commercial production, have enhanced understanding of the growth, reproduction, and physiological ecology Physiological ecology (animal)
A discipline that combines the study of physiological processes, the functions of living organisms and their parts, with ecological processes that connect the individual organism with population dynamics and community structure. of the clam under different environmental conditions, and thereby enabled rearing outside its normal habitat (Bourne Bourne, town (1990 pop. 16,064), Barnstable co., SE Mass., crossed by Cape Cod Canal; settled 1627, inc. 1884. Bourne Bridge (1935), across the canal, made the town an entry point to Cape Cod and a resort and commercial center. 1982, Beninger & Lucas 1984, Xie & Burnell 1994).
In contrast to a progressive increase in T. philippinarum production in coastal areas where the clam was introduced, a steady trend of decreasing annual production has been observed in indigenous habitats on the Korean coast, from approximately 74,000 tonnes in 1990 to around 38,000 tonnes in 2000 (Ministry of Maritime Affairs and Fisheries The Ministry of Maritime Affairs and Fisheries, or MOMAF, is a cabinet-level division of the government of South Korea. It oversees a variety of government offices, including the marine police. Republic of Korea 2005, National Fisheries fisheries. From earliest times and in practically all countries, fisheries have been of industrial and commercial importance. In the large N Atlantic fishing grounds off Newfoundland and Labrador, for example, European and North American fishing fleets have long Research and Development Institute 2004). The major causes of this decline have long been recognized and include habitat loss through reclamation of tidal flats, habitat disturbance by marine pollution, overexploitation of the species, mass mortality (main causes are unknown yet), and predation predation
Form of food getting in which one animal, the predator, eats an animal of another species, the prey, immediately after killing it or, in some cases, while it is still alive. Most predators are generalists; they eat a variety of prey species. of mud shrimp The term mud shrimp is used for a number of different mud-dwelling crustaceans:
The condition (flesh content) and biochemical composition of bivalves vary in time and space, because they are affected by exogenous Exogenous
Describes facts outside the control of the firm. Converse of endogenous. factors, such as water temperature and food availability, and endogenous endogenous /en·dog·e·nous/ (en-doj´e-nus) produced within or caused by factors within the organism.
1. Originating or produced within an organism, tissue, or cell. factors, such as energy demands for reproduction (Ansell & Trevallion 1967, Dare & Davies 1975, Newell & Bayne 1980, Navarro et al. 1989, Okumus& Stirling 1998, Kang et al. 2000). A close relationship has also been demonstrated between condition, the accumulation and utilization of nutrients, and the reproductive cycle in various bivalve species (Walne 1970, Gabbott 1975, Bayne 1976, Zandee et al. 1980, Ruiz et al. 1992, Ojea et al. 2004). Accordingly, seasonal variations in condition and biochemical composition of T. philippinarum can give a seasonal indication of its physiological or nutritional state under varying environmental conditions during the gametogenic cycle (Beninger & Lucas 1984, Robert et al. 1993, Marin et al. 2003, Meneghetti et al. 2004).
In this study we evaluated seasonal variations in condition, reproductive activity, and biochemical composition of the Manila clam, T. philippinarum, in natural and newly created sandy habitats.
MATERIALS AND METHODS
Study Site and Field Experiment Design
Jinju Bay is a small (about 25 km long and 13 km wide), shallow bay located on the southern coast of Korea. with a mean water depth of 4 m (Fig. 1). It is well protected by islands to the south and the water is well mixed by strong tidal currents. The tide is semidiurnal sem·i·di·ur·nal
1. Of, relating to, occurring, or performed during half a day.
2. Occurring or coming approximately once every 12 hours, as the tides.
3. with a maximum tidal range of 3.6 m on spring tide. Intertidal sediments in the bay are typically muddy sand, and about 411 ha have been developed for Manila clam culture. However, mud depth in the bay has increased up to 50 cm during the last two decades through sedimentation sedimentation
In geology, the process of deposition of a solid material from a state of suspension or solution in a fluid (usually air or water). Broadly defined it also includes deposits from glacial ice and materials collected under the effect of gravity alone, as in talus of suspended silt transported in intermittent freshwater pulses from the artificial dam on the Nam River, north of the bay. This has made the sediment muddier and thus reduced the area of clam habitat.
To assess if clam farming ground could be developed, sand of a total of 900 [m.sup.3] to a depth of 18 cm was added to the fine sediment surface of 5000 [m.sup.2] (50 m x 100 m) of a mudflat site on the west of the bay in March 2000. The sanded flat was tilled by raking nine months after addition of the sand. creating a silty silt
A sedimentary material consisting of very fine particles intermediate in size between sand and clay.
v. silt·ed, silt·ing, silts
v.intr. sand habitat. In May 2000, clam juveniles collected from natural beds were seeded onto three sites: the created silty sand habitat, a nonsanded mudflat area, and a natural habitat area. Juvenile clams of about 1500 kg were planted at each site. The period of immersion varied between 1 h (neap tides the lowest tides of the lunar month, which occur in the second and fourth quarters of the moon; - opposed to
See also: Neap ) and 5 h (spring tides) per tidal cycle at the three sites. Water depth was approximately 2 m at high tide, depending on the neap-spring tidal cycle. Sediment characteristics of the experimental sites, and initial density, shell length, and final density of the clam are presented in Table 1. Initial clam density was 153 [+ or -] 8 (SE). 112 [+ or -] 10, and 112 [+ or -] 10 individuals [m.sup.-2] at the non-sanded mudflat area, created silty sand habitat, and natural habitat, respectively. At the end of experiment, the clam density was 34 [+ or -] 3 (SE) and 23 [+ or -] 1 (SE) individuals [m.sup.-2] at the created silty sand habitat and natural habitat, respectively. Survival rates were over 90% of the initial density at those two sites two months after seeding, but all the clams at the nonsanded mudflat area had disappeared during that period. Therefore, further monthly sampling was only conducted at the created silty sand habitat (the treatment site) and natural habitat (the control site).
[FIGURE 1 OMITTED]
Clam Collection and Biometric Measurement
Clams were collected monthly at the treatment and control sites from May 2000 to October 2001. Clams of similar size were sampled to minimize compositional variations because of age (size) class differences and to assess seasonal variation in clam activities (Fig. 2). About 60 individuals were collected at each site using a manual rake. Specimens were transported to the laboratory and placed overnight in filtered seawater seawater
Water that makes up the oceans and seas. Seawater is a complex mixture of 96.5% water, 2.5% salts, and small amounts of other substances. Much of the world's magnesium is recovered from seawater, as are large quantities of bromine. at in situ In place. When something is "in situ," it is in its original location. temperature, to ensure gut evacuation and removal of fecal fecal /fe·cal/ (fe´k'l) pertaining to or of the nature of feces.
Relating to or composed of feces.
pertaining to or of the nature of feces. contents. After rinsing, the weight of each clam was determined and the shell length, width, and height were measured to the nearest 0.05 mm using Vernier calipers See Vernier.
a gauge with a graduated bar and a sliding jaw bearing a vernier, used for accurate measurements.
See also: Calipers Vernier . The clams were then dissected dis·sect·ed
1. Botany Divided into many deep, narrow segments: dissected leaves.
2. Geology Cut by irregular valleys and hills.
Adj. 1. and the sex of each determined by a smear. Tissue dry weight and biochemical composition was determined for 15-20 specimens of each sex, and a further 10 specimens of each sex were used for histological his·tol·o·gy
n. pl. his·tol·o·gies
1. The anatomical study of the microscopic structure of animal and plant tissues.
2. The microscopic structure of tissue. analysis. The shell of each clam was dried at 60[degrees]C for approximately 72 h, and the shell weight recorded. Tissues dissected for biochemical analysis were freeze-dried and the tissue dry weight of each individual was determined by subtracting the water content mass from wet tissue mass. The dried tissues were ground to powder with a mortar and pestle A mortar and pestle is a tool used to crush, grind, and mix substances. The pestle is a heavy stick whose end is used for pounding and grinding, and the mortar is a bowl. The substance is ground between the pestle and the mortar. and an aliquot aliquot (al-ee-kwoh) adj. a definite fractional share, usually applied when dividing and distributing a dead person's estate or trust assets. (See: share) was heated at 450[degrees]C for 24 h to determine ash weight. The remaining dry tissue was stored at -30[degrees]C in a refrigerator for later biochemical analysis.
On each sampling occasion at each site the temperature and salinity of surface and bottom waters were measured at high tide using a CTD CTD 1 Connective tissue disease, see there 2 Cumulative trauma disorder, see there meter (Seabird Electronics, Inc.). For bottom waters, 5 L was pumped from 1 m above the bottom, screened through a 180 [micro]m Nitex mesh to eliminate zooplankton zooplankton: see marine biology.
Small floating or weakly swimming animals that drift with water currents and, with phytoplankton, make up the planktonic food supply on which almost all oceanic organisms ultimately depend (see and large particles, and collected in acid-washed plastic bottles. Water samples were kept on ice in the dark until filtered on pre-combusted Whatman GF/F glass-fiber filters (47 mm, 0.7 [micro]m pore size). Filters for chlorophyll a Noun 1. chlorophyll a - a blue-black plant pigment having a blue-green alcohol solution; found in all higher plants
chlorophyl, chlorophyll - any of a group of green pigments found in photosynthetic organisms; there are four naturally occurring forms analysis were stored at -80[degrees]C, and filters for suspended particulate matter particulate matter
n. Abbr. PM
Material suspended in the air in the form of minute solid particles or liquid droplets, especially when considered as an atmospheric pollutant.
Noun 1. were dried at 60[degrees]C overnight and then placed in a dessicator.
Chlorophyll a concentration was determined from acetone acetone (ăs`ĭtōn), dimethyl ketone (dīmĕth`əl kē`tōn), or 2-propanone (prō`pənōn), CH3COCH3 extracts using a fluorometric method according to according to
1. As stated or indicated by; on the authority of: according to historians.
2. In keeping with: according to instructions.
3. Holm-Hansen et al. (1965), using a fluorometer fluorometer /flu·o·rom·e·ter/ (fldbobr-rom´e-ter) the instrument used in fluorometry, consisting of an energy source (e.g., a mercury arc lamp or xenon lamp) to induce fluorescence, filters or monochromators for selection of the (Turner Designs Model 10 AU 005). Total suspended particulate matter (SPM SPM - Sequential Parlog Machine ) was determined from filter weights before and after drying, following filtration of a known volume of water.
Condition and Reproductive Cycle
The condition index was calculated from the dry weight of tissue and shell according to the formula: Condition = [tissue dry weight (mg)/dry shell weight (mg)] x 100 (Lucas and Beninger 1985).
For 10 specimens of each sex, the gonads were fixed in Bouin solution, embedded in paraffin paraffin, white, more-or-less translucent, odorless, tasteless, waxy solid. It melts between 47°C; and 65°C; and is insoluble in water but soluble in ether, benzene, and certain esters. , sectioned at 5 [micro]m, and stained with iron hematoxylin-eosin (Humason 1962). The gametogenic stage was classified (Chung et al. 1994, Sbrenna & Campioni 1994), and scored on a 0-4 scale according to Mann's (1979a) scheme: stage 0 = inactive, stage 1 = early active, stage 2 = late active, stage 3 = ripe, stage 4 = spawning-spent). The arithmetic means (mathematics) arithmetic mean - The mean of a list of N numbers calculated by dividing their sum by N. The arithmetic mean is appropriate for sets of numbers that are added together or that form an arithmetic series. of the individual scores of whole specimens were recorded as the gonadal gonadal
pertaining to or arising from a gonad. See also testicular, ovarian.
cords formed by epithelial cells which migrate from the mesonephric tubules in the embryo to the gonadal ridge and establish the indifferent maturity index (GMI GMI Governance Metrics International (New York, New York)
GMI Giant Magneto-Impedance
GMI Global MSF Interoperability
GMI General Motors Institute
GMI General Mills, Inc. ) for each sampling occasion (see details in Meneghetti et al. 2004).
Powdered sample (5-10 mg) was used for biochemical analysis. Protein content was determined using the method of Lowry et al. (1951) after alkaline hydrolysis hydrolysis (hīdrŏl`ĭsĭs), chemical reaction of a compound with water, usually resulting in the formation of one or more new compounds. with 0.5 N NaOH at 30[degrees]C for 24 h. Carbohydrate and glycogen glycogen (glī`kəjən), starchlike polysaccharide (see carbohydrate) that is found in the liver and muscles of humans and the higher animals and in the cells of the lower animals. were extracted in 15% trichloroacetic acid trichloroacetic acid /tri·chlo·ro·ace·tic ac·id/ (tri-klor?o-ah-se´tik) an extremely caustic acid, used in clinical chemistry to precipitate proteins and applied topically in chemabrasion and to remove warts. and determined as glucose following the phenol-sulfuric acid method (Dubois et al. 1956). Glycogen was quantified after precipitation with 100% ethanol. Extraction of total lipid was performed in a mixture of chloroform chloroform (klôr`əfôrm) or trichloromethane (trī'klôrōmĕth`ān), CHCl3 and methanol methanol, methyl alcohol, or wood alcohol, CH3OH, a colorless, flammable liquid that is miscible with water in all proportions. Methanol is a monohydric alcohol. It melts at −97. (Bligh & Dyer 1959) and lipid content determined following the method of Marsh and Weinstein (1966).
[FIGURE 2 OMITTED]
To evaluate the physiological state Noun 1. physiological state - the condition or state of the body or bodily functions
physical condition, physiological condition
wakefulness - a periodic state during which you are conscious and aware of the world; "consciousness during wakefulness in a sane of T. philippinarum independent of growth, absolute values for tissue dry weight were standardized to that of a clam (standard animal) of 31.5 mm in shell length (the mean of all specimens analyzed) and compared for each sampling occasion. Allometric al·lom·e·try
The study of the change in proportion of various parts of an organism as a consequence of growth.
al equations of tissue dry weight against shell length at each sampling were determined by linear regression Linear regression
A statistical technique for fitting a straight line to a set of data points. analysis following logarithmic logarithmic
pertaining to logarithm.
when the logs of two variables plotted against each other create a straight line. transformation (base 10). The same analysis was used to relate the weight of biochemical constituents and ash to the tissue dry weight. All regressions were statistically significant (P < 0.01). Dry weights of biochemical constituents were then computed for a given shell length by substituting appropriate values of tissue weight in the regression equations Regression equation
An equation that describes the average relationship between a dependent variable and a set of explanatory variables. referred to above (see details in Navarro et al. 1989). The results of biochemical analysis were then expressed in mg per standard animal.
Stable Isotopes stable isotope
An isotope of an element that shows no tendency to undergo radioactive breakdown.
The clam tissue [[delta].sup.13]C and [[delta].sup.15]N were measured for at least four individuals, sampled bimonthly bi·month·ly
1. Happening every two months.
2. Happening twice a month; semimonthly.
1. Once every two months.
2. Twice a month; semimonthly.
n. pl. at each site as described above. Marine particulate par·tic·u·late
Of or occurring in the form of fine particles.
A particulate substance.
composed of separate particles. organic matter (POM) and riverine riv·er·ine
1. Relating to or resembling a river.
2. Located on or inhabiting the banks of a river; riparian: "Members of a riverine tribe ... POM were collected bimonthly at the bay mouth and downstream of the Nam River. For sampling POM at each site, about 20 L of water was prefiltered in situ with a 250-[micro]m screen to remove large particles and transported to the laboratory as soon as possible. The particulates in this prefiltered water were concentrated onto a precombusted Whatman GF/F filter in the laboratory, treated with 2-3 drops of 10% HCl, and then rinsed with distilled water Noun 1. distilled water - water that has been purified by distillation
H2O, water - binary compound that occurs at room temperature as a clear colorless odorless tasteless liquid; freezes into ice below 0 degrees centigrade and boils above 100 degrees centigrade; . For each POM sampling, microphytobenthos was collected by scraping the visible mat of benthic ben·thos
1. The collection of organisms living on or in sea or lake bottoms.
2. The bottom of a sea or lake.
[Greek. diatoms diatoms
a series of unicellular algae, microscopic in size, with cell walls containing silica. Members of the family Diatomaceae. Their remains accumulate as geological deposits and are mined. See diatomaceous earth. from the sediment surface. This was extracted and prepared for isotope analysis Isotope analysis is the identification of isotopic signature, the distribution of certain stable isotopes and chemical elements within chemical compounds. This can be applied to a food web to make it possible to draw direct inferences regarding diet, trophic level, and subsistence. according to Couch's procedure (1989), as described by Riera and Richard (1996). These pretreated samples were kept frozen until analysis, freeze-dried at -70[degrees]C, and then loaded into tin capsules prior to isotope analysis.
Values for [[delta].sup.13]C and [[delta].sup.15]N were obtained using a CHN CHN China
CHN Canadian Health Network
CHN Coalition on Human Needs
CHN California Homeschool Network
CHN Cleveland Housing Network
CHN Center for Human Nutrition
CHN Carbon, Hydrogen, Nitrogen
CHN Community Health Nurse elemental analyzer (EuroVector 3000 Series, Italy) combined with a continuous-flow isotope ratio mass spectrometer spectrometer
Device for detecting and analyzing wavelengths of electromagnetic radiation, commonly used for molecular spectroscopy; more broadly, any of various instruments in which an emission (as of electromagnetic radiation or particles) is spread out according to some (GV Instruments, IsoPrime, UK). All isotope ratios were expressed as the relative per mill ([per thousand]) difference between the sample and conventional standard reference materials (PeeDee Belemnite bel·em·nite
A cone-shaped, fossilized internal shell of any of an extinct genus of cephalopods related to the cuttlefish.
[New Latin belemn carbonate and [N.sub.2] in air) as follows: [delta]X = [([R.sub.sample]/[R.sub.standard]) - 1] x [10.sup.3], where X is [sup.13]C or [sup.15]N, and R is the corresponding ratio of [sup.13]C:[sup.12]C or [sup.15]N:[sup.14]N. A laboratory internal standard (peptone peptone /pep·tone/ (pep´ton) a derived protein, or a mixture of cleavage products produced by partial hydrolysis of native protein.pepton´ic
n. , Merke) was run every sixth sample. Measurement precision based on the standard deviation In statistics, the average amount a number varies from the average number in a series of numbers.
(statistics) standard deviation - (SD) A measure of the range of values in a set of numbers. of 20 replicates of the internal standard was within 0.2[per thousand] for both isotope pairs. Two replicates were analyzed from each sample of ground clam tissue.
Commercially available software was used to analyze the experimental results (SPSS A statistical package from SPSS, Inc., Chicago (www.spss.com) that runs on PCs, most mainframes and minis and is used extensively in marketing research. It provides over 50 statistical processes, including regression analysis, correlation and analysis of variance. package, Chicago, IL). Data were tested for normality normality, in chemistry: see concentration. using the Shapiro-Wilk procedure. Leven test was used to check the homogeneity Homogeneity
The degree to which items are similar. of variance among data. A paired sample t-test for paired comparisons was used to test the significance of variations in environmental parameters between sites and to determine differences in condition index, tissue dry weight, and biochemical components between sexes or sites during the study period. The arcsine transformation was applied to percentage data, such as condition index, prior to analysis. Analysis of variance (ANOVA anova
see analysis of variance.
ANOVA Analysis of variance, see there , two-way) with Tukey multiple comparison test was performed to determine differences in [[delta].sup.13]C and [[delta].sup.15]N values of the Manila clam tissues between sites and among sampling months.
Temperature, Salinity, and Potential Food
Monthly mean water temperature showed a seasonal cycle typical of temperate zones, with maximum temperatures (22.6[degrees]C to 25.1[degrees]C) in July-September and minimum temperatures (7.4[degrees]C to 8.0[degrees]C) in January-February (Fig. 3). Salinity varied inversely with a summer minimum of less than 30 PSU PSU - power supply unit in 2000 and 31 PSU in 2001, according to the monsoonal climate and constant values at around 33 PSU for the major part of the year. No differences in temperature and salinity were found between sites (paired t-test, P = 0.798 and 0.067).
Monthly mean SPM concentration fluctuated between 6.9 and 19.0 mg [L.sup.-1] (Fig. 4A). Although relatively high concentrations of more than 16 mg [L.sup.-1] were recorded during May to July 2001, concentrations were highly variable. Although chlorophyll a concentration peaked in summer in 2000, coincident co·in·ci·dent
1. Occupying the same area in space or happening at the same time: a series of coincident events. See Synonyms at contemporary.
2. with the period of salinity minima, its seasonal trend was not clear (Fig. 4B). Rather, chlorophyll a concentration was also characterized by irregular peaks as shown in variations of SPM concentration. Both SPM and chlorophyll a values did not differ significantly between sites (P = 0.422 and 0.559).
[FIGURE 3 OMITTED]
Condition and Reproductive Cycle
Because of the possibility of sexual differentiation sexual differentiation See Hermaphroditism, hirsutism, Müllerian ducts, Precocious puberty, Pseudoprecocious puberty, Tanner staging, Testis-determining factor, Virilization, Wolffian ducts, XXX, XXY, XXXY, XYY syndromes, Y Chromosome. in biochemical composition, as shown in the clam Ruditapes decussates (L.) (Perez Camacho et al. 2003), all analyses in this study were carried out separately for each sex. Seasonal variations in condition index were very similar between control and treatment sites, and the data showed a clear seasonal pattern (Fig. 5) that was very similar between sexes. The maximum values in condition index occurred in May 2000 and were followed by a progressive decline throughout the summer-autumn period to minimum levels in October. After November a subsequent increase was observed, which peaked in March to April 2001. This spring peak was followed by a summer decline. No statistical difference in condition index was found between females and males of each population (P = 0.583 and 0.113 for control and treatment sites, respectively) or between sites (P = 0.466 and 0.791 for female and male, respectively).
Values for GMI also exhibited clear seasonality that was similar between control and treatment sites (Fig. 6). This was attributed to sexual maturation of the clams. In both years, the Years, The
the seven decades of Eleanor Pargiter’s life. [Br. Lit.: Benét, 1109]
See : Time development of gonadal tissue was initiated in both sexes in January and peaked during May to July, when the condition index values started to decline. Gametogenic stage 4 can be used as the criterion for the beginning and end of spawning. The appearance and disappearance of stage 4 coincided with the period of condition decline, indicating that spawning began in May and continued until October. Spawning was followed by a sexual resting period in November to December.
[FIGURE 4 OMITTED]
[FIGURE 5 OMITTED]
Tissue Dry Weight
The tissue dry weight of a standard animal was calculated for each sex in the control and treatment groups (Fig. 7). There was a remarkable seasonal variation in tissue dry weight in both groups, the patterns being similar to those of the condition index. The dry weight of soft tissues decreased steadily from maximum levels in May 2000 to minimum levels in October, coincident with the spawning period, and increased progressively to spring 2001, when values peaked. This was followed by a gradual decrease to the end of the experiment in October 2001. Differences in tissue dry weight of standard animals were not statistically significant between females and males of each population (P = 0.150 and 0.727 for control and treatment sites) or between sites (P = 0.145 and 0.877 for female and male).
Gross Biochemical Composition
Seasonal variations in gross biochemical composition of standard animals showed similar patterns between sexes of each population or sites, with clear seasonal trends (Fig. 8). Seasonal patterns in absolute values for all biochemical components (protein, carbohydrate, and lipid) were very similar and followed those of tissue dry weight of standard animals and the condition index for each population.
Protein peaked at the beginning of the study in May 2000 and in April 2001. Sharp declines occurred in June 2000 and from August to October, when minimum values were detected. Loss in protein content correlated with spawning. Values then increased gradually to peak in March to April 2001, followed by a sudden decline in May and July to October. No statistical difference was found in the protein value of standard animals between females and males of each population (P = 0.148 and 0.811 for control and treatment sites) or between sites (P = 0.121 and 0.182 for female and male).
A similar seasonal pattern for carbohydrate and glycogen indicated that carbohydrate content of the clams depends on glycogen mobilization. Similar to protein, a carbohydrate peak occurred in May 2000 and was followed by a sudden decrease from June until October to November. Carbohydrate (glycogen) reserves were quickly restored during the winter to spring and decreased again through the summer to a minimum in October 2001. A paired t-test showed no significant difference in carbohydrate and glycogen values between females and males of each population (P = 0.693 and 0.820 for carbohydrate for control and treatment sites); 0.123 and 0.135 for glycogen) or between sites (P = 0.827 and 0.727 for carbohydrate for females and males; 0.199 and 0.142 for glycogen, respectively).
Lipid values were lowest in October to November and highest in spring, displaying a similar seasonal pattern to protein and carbohydrate. There was no difference in the lipid value of standard female and male clams between each population (P = 0.094 and 0.331 for control and treatment sites) or between sites (P = 0.389 and 0.341 for female and male).
Although peak values of ash content were observed in spring, its seasonal pattern was less pronounced than other biochemical variables. No significant difference in ash value of standard animals was found between females and males of each population (P = 0.141 and 0.191 for control and treatment sites) or between sites (P = 0.210 and 0.090, for females and males).
Stable Isotope Ratios for Clams and Potential Food Sources
Values for [[delta].sup.13]C in muscle tissue of clams averaged -17.4 [+ or -] 0.6[per thousand] (SD, n = 26) and -17.6 [+ or -] 0.6[per thousand] (n = 25) at the control and treatment sites, respectively (Table 2). There were no significant differences in [[delta].sup.13]C values between sites and among the sampling months (two-way ANOVA, [F.sub.(1, 39)] = 0.059, P = 0.809 for site; [F.sub.5, 39] = 2.318, P = 0.062 for month). Values for [[delta].sup.15]N averaged 10.8 [+ or -] 0.8[per thousand] (n = 23) and 10.5 [+ or -] 0.8[per thousand] (n = 23) at the control and treatment sites, respectively (Table 2), with no significant difference between sites (two-way ANOVA, [F.sub.5, 34] = 20.186, P < 0.001). However, the [[delta].sup.15]N values showed significant seasonal differences, being about 2[per thousand] more depleted de·plete
tr.v. de·plet·ed, de·plet·ing, de·pletes
To decrease the fullness of; use up or empty out.
[Latin d in June 2001 than in August, October, and December 2000 for both sites ([F.sub.5, 39] = 2.318, P = 0.062; Tukey HSD HSD Human Services Department
HSD High Speed Data
HSD Hillsboro School District (Hillsboro, OR)
HSD Hybrid Synergy Drive (Toyota/Lexus)
HSD High School Diploma
HSD Historical Society of Delaware test, P < 0.05). Although significant seasonal changes in both [[delta].sup.13]C and [[delta].sup.15]N values for microphytobenthos, marine POM, and riverine POM were detected, the stable isotope compositions of these three potential food sources were clearly distinguished: the average values (n = 12) were, respectively, -14.5 [+ or -] 1.6[per thousand], 21.1 [+ or -] 1.1[per thousand], and -27.8 [+ or -] 2.7[per thousand] for [[delta].sup.13]C, and 8.9 [+ or -] 0.9[per thousand], 5.7 [+ or -] 1.3[per thousand], and 9.0 [+ or -] 0.8[per thousand] for [[delta].sup.15]N (Table 2; Fig. 9).
According to a dual plot of [[delta].sup.13]C and [[delta].sup.15]N deviations measured for the clams and their potential food sources (Fig. 9), the dietary components of the clams appeared to be primarily composed of microphytobenthos and marine POM throughout the year. Figure 10 presents the relative biomass contributions of the three dietary sources, calculated from a linear mixing model based on mass balance equations using [[delta].sup.13]C and [[delta].sup.15]N values (the IsoSource model, Phillips & Gregg 2003). The results confirmed that a major part of clam biomass for both sites was derived from microphytobenthos and marine POM. Microphytobenthos contribution tended to be relatively constant from August 2000 to April 2001, and then sharply decreased to June. Conversely, the contribution of marine POM progressively increased from October 2000 to June 2001. The dietary contribution of riverine POM ranged between 11% and 17% in August and October 2000, but it was negligible throughout the rest of the year.
[FIGURE 6 OMITTED]
The condition index of marine bivalves summarizes the physiological activity of the animals under given environmental conditions, and its fluctuation has important implications for cultivation and harvesting strategies for bivalves (Lucas & Beninger 1985, Okumus& Stirling 1998). The primary objective in this study was to compare the condition of Manila clams (T. philippinarum) reared in natural and artificially created habitat in relation to gametogenic processes, and accumulation and mobilization of reserve materials, as well as to evaluate habitat functions of the created farming grounds for this species. The condition index had a seasonal cycle at both the treatment and the control site, with minimum values in autumn (October to November) rising to peak levels in early spring (May 2000 and March to April 2001) (Fig. 5). No difference was evident in the index level between clams from the natural and created habitat. As the condition of bivalves reflects changes of physiological status dependent on environmental factors including food supply and temperature, this result indicates that clam stocks from created silty sand habitat can have biological traits similar to those from natural clam habitat and provide a good quality product for market.
In this study, marine sand transported by a large barge was sprinkled on the muddy sediments during the high slack water slack water
1. A period of cessation in the strong flow of a current of water, especially at high or low tide.
2. An area in a sea or river unaffected by currents; still water.
Noun 1. period and then flattened flat·ten
v. flat·tened, flat·ten·ing, flat·tens
1. To make flat or flatter.
2. To knock down; lay low: The boxer was flattened with one punch. during the ebb tide ebb tide
The receding or outgoing tide; the period between high water and the succeeding low water.
The period between high tide and low tide during which water flows away from the shore. . Adding sand made the muddy substrate coarser (Table 1). Monthly monitoring of the sediment characteristics showed that chemical oxygen demand (7.53-11.80 mg [g.sup.-1]), hydrogen sulfide hydrogen sulfide, chemical compound, H2S, a colorless, extremely poisonous gas that has a very disagreeable odor, much like that of rotten eggs. It is slightly soluble in water and is soluble in carbon disulfide. (0.00-0.08 mg [g.sup.-1]), and organic content (1.0-7.7%) of coarser sediments in the created habitats were much lower than those (9.27-32.58 mg [g.sup.-1], 0.00-0.10 mg [g.sup.-l], and 7.0-8.1%, respectively) in muddy sediments (data not shown in Results). Clam-farming ground exposed to environmental conditions similar to those of natural clam habitat was created by adding sand to the muddy substrate, and none of the hydrological hy·drol·o·gy
The scientific study of the properties, distribution, and effects of water on the earth's surface, in the soil and underlying rocks, and in the atmosphere. and sedimentary parameters evaluated showed significant differences between the natural and created habitats (Figs. 3 and 4, Table 1). Although most differences were caused by reproductive and physiological characteristics of clams rather than regional differences in environmental conditions, environmentally stressful conditions can result in differences in physiological status of marine bivalves in time and space (Navarro et al. 1989, Robert et al. 1993, Kang et al. 2000, Marin et al. 2003). Accordingly, the comparable environmental conditions between the natural and created habitat were probably responsible for the similarity in timing and level of the maximum and minimum values for the condition index of clams in this study.
[FIGURE 7 OMITTED]
Spring maxima in condition found during this investigation have also been observed in European populations (Beninger & Lucas 1984, Marin et al. 2003). Biometric data revealed that the maximum condition value was considerably greater in both stocks in the spring of 2000 than in 2001. Similar results were obtained by Beninger and Lucas (1984) in T. philippinarum from the north Atlantic coast of France. This may be explained by the diminishing growth rate with size (age) in marine bivalves (Ohba 1959, Beninger & Lucas 1984, and references therein). The spring maxima in condition were followed by a progressive decline during summer and autumn caused by spawning, after which clam condition recovered rapidly in parallel to accumulation of energy the storing of energy by means of weights lifted or masses put in motion; electricity stored.
See also: Accumulation reserves in late autumn to winter 2000. The timing of condition minima recorded during this study appeared to be somewhat different from those observed in European clam populations. In Brittany (France), Beninger and Lucas (1984) observed only a partial condition recovery in spring because of physiological stress during the autumn and winter. In the Lagoon of Venice, Marin et al. (2003) also found condition recovery from February throughout spring to summer, because of the decrease in water temperature and food availability in autumn to winter. In other words Adv. 1. in other words - otherwise stated; "in other words, we are broke"
put differently , minimum values in condition index were recorded during winter in European waters. These differences in timing of condition minima and recovery suggest that the clams at the study sites had a positive energy balance during late autumn and winter, as indicated by the accumulation of energy reserves after spawning.
Strong positive correlations between condition index and GMI values (control: r = 0.752 and 0.780 for female and male, P < 0.001 for both; treatment: r = 0.748 and 0.724, P < 0.001) indicated a close relationship between the gametogenic cycle and condition. There was no apparent difference in stages of gonadal maturation between sites (Fig. 6). After a brief inactive period in late autumn (November to December), gametogenesis Gametogenesis
The production of gametes, either eggs by the female or sperm by the male, through a process involving meiosis. In animals, the cells which will ultimately differentiate into eggs and sperm arise from primordial germ cells set aside from the was initiated in January. Ripe stage was reached in spring and was followed by spawning, which began in May and lasted throughout summer until October. Mean water temperature was 7.6[degrees]C in January 2001 and 14.3[degrees]C in May, comparable to minimum temperatures of 8[degrees]C for gonadal activity and 14[degrees]C for spawning of T. philippinarum (Mann 1979b). The reproductive cycle of T. philippinarum observed during this investigation is very similar to that of Mediterranean populations (Sbrenna & Campioni 1994, Meneghetti et al. 2004) and a Japanese population (Ohba 1959). However, the onset of gametogenesis and gamete gamete (găm`ēt): see reproduction. ripening ripening
said of meat. See curing. in northern European populations (Beninger & Lucas 1984, Xie & Burnell 1994) and Vostok Bay populations of the northwestern part of the East/Japan Sea (Ponurovsky & Yakovlev 1992) is delayed compared with populations in warmer climates. Xie and Burnell (1994) speculated that this time lag could be explained by a time to temperature effect.
Continuous or multiple spawning during summer has been observed worldwide (Ponurovsky & Yakovlev 1992, Sbrenna & Campioni 1994, and references therein). In the present investigation, it is difficult to determine the precise timing and intensity of spawning from a monthly sampling strategy. The seasonal cycles of gametogenesis and the amplitude of seasonal fluctuations of standard animal tissue dry weight was quite similar between the natural and created habitat. The loss of tissue weight observed during the spring-summer period appeared to coincide with spawning time as seen in the seasonal variations in the sexual maturation process and the GMI values (Figs. 6 and 7). The rapid decrease in standard animal tissue dry weight during the spring to summer period suggests two spawning events. The spawning pattern of the Manila clam is known to be highly variable, with one spawning period reported in Washington State and Ireland (Holland & Chew 1974, Xie & Burnell 1994, respectively); two spawning peaks in Japan (Tanaka 1954, Ohba 1959), France (Beninger & Lucas 1984) and Italy (Sbrenna & Campioni 1994, Meneghetti et al. 2004); and three spawning events in southwest Spain (Sarasquete et al. 1990). The timing and intensity of gonadal development and spawning is known to vary locally, seasonally, and interannually (Beninger & Lucas 1984, Navarro et al. 1989, Sbrenna & Campioni 1994, Kang et al. 2000, Meneghetti et al. 2004). These studies demonstrated that temperature is not the only factor acting on gonad gonad /go·nad/ (go´nad) a gamete-producing gland; an ovary or testis.gonad´algonad´ial
indifferent gonad the sexually undifferentiated gonad of the early embryo. development and the beginning of spawning, and that local or temporal variations in gonadal maturation of bivalves are closely related to variations in nutritional conditions (that is, food availability). Therefore, the gametogenic cycle observed during this investigation appears to be explained by a seasonal cycle in accumulation and mobilization of reserve materials in clams in relation to variability in trophic trophic /tro·phic/ (tro´fik) (trof´ik) pertaining to nutrition.
Of, relating to, or characterized by nutrition. conditions.
[FIGURE 8 OMITTED]
Seasonal patterns and levels of tissue dry weight and biochemical components in clams were quite similar in the natural and created habitat (Figs. 7 and 8), indicating that clams reared in created habitats can have the same physiological characteristics as those in natural habitats. Seasonal variations in the absolute values of biochemical components paralleled those of tissue dry weights in clams at both sites. Seasonal cycles in tissue dry weight of standard animals seemed to reflect the reproductive cycle, with weight being maximal prior to the spring to summer spawning, and then decreasing simultaneously with spawning. Protein constitutes the major organic component of gametes in T. philippinarum (Beninger & Lucas 1984). Somatic somatic /so·mat·ic/ (so-mat´ik)
1. pertaining to or characteristic of the soma or body.
2. pertaining to the body wall in contrast to the viscera.
adj. proteins are also mobilized during gametogenesis, acting as the predominant respiratory substrate during this period (Mann & Glomb 1978, Adachi 1979; see also Beninger & Lucas 1984, Marin et al. 2003). As expected from these studies, the absolute values for protein in clams in this study peaked during the spring-summer spawning period (Fig. 8). A sharp decline in May to June 2000 and April to May 2001 indicated the first spawning during these periods. After this first spawning, there was a rapid recovery in protein content and then a slow decrease until October, indicating the second spawning event.
[FIGURE 9 OMITTED]
Seasonal variation in absolute values for lipid was very similar to those of protein. It is commonly accepted that glycogen reserves are converted into lipids, which are a major component of bivalve oocytes (Holland 1978, Gabbott 1983, Beninger & Lucas 1984, Marin et al. 2003). Accordingly, the maximum values detected prior to spawning, and the minimum after, may be explained by the above hypothesis. As observed in the seasonal variation in protein, there was a rapid recovery in lipid content after the first spawning. Somatic proteins and lipids also serve as energy reserves in T. philippinarum under conditions of nutritional stress and energy imbalance (Beninger & Lucas 1984, Marin et al. 2003). After the spawning period in this study, tissue dry weight began to increase without a period of decrease during inactive gonadal phase (no oocytes) in winter, in contrast to European populations (Beninger & Lucas 1984, Marin et al. 2003). Because the absolute values of all biochemical components also increased during this period, it is difficult from this study to confirm the role of proteins and lipids as maintenance energy reserves.
Seasonal patterns of carbohydrate in clams from the sampling sites could be explained by glycogen patterns. Carbohydrate and glycogen peaks in May 2000 were followed by a sudden decrease as a consequence of spawning. In contrast to protein and lipid, glycogen recovery was not detected after the first spawning in both 2000 and 2001. Glycogen has long been considered to be the main energy reserve for both the formation of gametes and the maintenance of adult bivalves during periods of nutritional stress (see Beninger & Lucas 1984). The results presented here support this conclusion and also the conversion of glycogen into lipids, as previously discussed. The absolute values for glycogen were lowest at the end of spawning and then quickly recovered throughout winter to spring. In general, the recovery and accumulation of glycogen in bivalves is related to good conditions of food availability (Ansell & Trevallion 1967, Newell & Bayne 1980, Navarro et al. 1989, Okumus& Stirling 1998, Kang et al. 2000). Beninger and Lucas (1984) showed that a failure to constitute energy reserves in autumn after spawning, and the exhaustion of reserves caused by energy imbalances in winter, resulted in a delay in the recovery of condition and the maturation of gonads in Brittany (France) populations of T. philippinarum. On the other hand, Sbrenna and Campioni (1994), and Meneghetti et al. (2004) suggested that high accumulation of reserve materials, because of high food availability from late winter to early spring, leads to gamete development in winter and spawning in May in lagoon systems of Italy. Thus, the recovery of glycogen reserves throughout winter--spring in this study indicates that the Manila clams had sufficient food supply to enable resumption of gonadal maturation and spawning in May.
[FIGURE 10 OMITTED]
Comparison of isotopic signatures of T. philippinarum tissues with those of potential food resources confirmed the contribution of microphytobenthos to the clam diet in both the natural and the created habitat (Fig. 10). The POM and chlorophyll a data did not show any seasonal trend, indicating no apparent seasonality in the trophic conditions of ambient waters. Irregular peaks in SPM and chlorophyll a values, as well as the isotopic data. suggest that the nutritional conditions of waters were strongly influenced by resuspension caused by tide and wind. Therefore, despite an important role of phytoplankton in the clam diet throughout the year, food availability to meet energy requirements in this study area might be maintained by resuspension of microphytobenthos, even during the winter to spring period. The high availability Also called "RAS" (reliability, availability, serviceability) or "fault resilient," it refers to a multiprocessing system that can quickly recover from a failure. There may be a minute or two of downtime while one system switches over to another, but processing will continue. of food because of the summer phytoplankton blooms may have provided energy requirements for the rapid recovery of reserve materials after the first spawning as well as for the second spawning in summer. The isotopic signatures of clam tissues showed that the dietary contribution of organic matter from marine POM (largely phytoplankton) increased markedly in late spring to summer 2001. Phytoplankton blooms caused by heavy rain and nutrient input, which are concentrated during late spring to early summer, are well-known phenomena in coastal regions with a monsoonal climate, as occurs on the Korean peninsula (Kang et al. 2000, Park et al. 2001). Although limited to late summer, a dietary contribution from riverine POM was detected. This is consistent with the conclusion of Kasai et al. (2004) that Manila clams select marine POM (phytoplankton and microphytobenthos) from the organic matter available in their habitat, and the contribution of terrestrial material increases temporarily during rainfall. Finally, our biochemical and isotopic data indicated that food availability in the study area was mostly dependent on resuspension of microphytobenthos, along with seasonal dynamics of phytoplankton.
In summary, clams reared in newly created or natural habitats had similar patterns and levels in condition and tissue dry weight. The similar biochemical compositions and reproductive cycles for clams in the two habitats are likely to be a consequence of similarities in environmental conditions, and thereby food availability. The results show that the newly created sandy habitats may provide habitat functions that enable Manila clams to have biological cycles similar to those in natural habitats. These results confirm the conclusion of Melia et al. (2004) that rearing sites with high sand content are preferable in terms of growth and maximum attainable size of Manila clams. This initial experiment primarily tested whether Manila clams can adapt to created sandy habitats. For commercially sustainable exploitation and better yield of T. philippinarum in the future, more detailed studies of the effect of stock density and deposition rate of silt modifying the substrate are needed.
The authors thank H. J. Park, J. H. Kwak, and E. J. Jang for their technical assistance and the anonymous referees for their reviewing and critical comments on the manuscript. This work was supported for two years by Pusan National University History
Pusan National University (PNU) was founded on May 1946 in Pusan, Korea's second largest metropolis, by Korean government,which has been established five months earlier than Seoul National University in Seoul. Research Grant.
Adachi, K. 1979. Seasonal changes of the protein level in the adductor muscle Noun 1. adductor muscle - a muscle that draws a body part toward the median line
skeletal muscle, striated muscle - a muscle that is connected at either or both ends to a bone and so move parts of the skeleton; a muscle that is characterized by of the clam, Tapes philippinarum (Adams and Reeve) with special reference to the reproductive seasons. Comp. Biochem. Physiol. 64:85-89.
Ansell, A. D. & A. Trevallion. 1967. Studies on Tellina tenuis ten·u·is
n. pl. ten·u·es Linguistics
1. A voiceless stop.
2. A voiceless unaspirated stop in ancient Greek. de Costa: I. Seasonal growth and biochemical cycle. J. Exp. Mar. Biol. Ecol. 1:220-235.
Bayne, B. L. 1976. Aspects of reproduction in bivalve mollusks. In: M. L. Wiley, editor. Estuarine es·tu·a·rine
1. Of, relating to, or found in an estuary.
2. Geology Formed or deposited in an estuary.
Adj. 1. estuarine - of or relating to or found in estuaries
estuarial processes. New York New York, state, United States
New York, Middle Atlantic state of the United States. It is bordered by Vermont, Massachusetts, Connecticut, and the Atlantic Ocean (E), New Jersey and Pennsylvania (S), Lakes Erie and Ontario and the Canadian province of : Academic Press. pp. 432-448.
Beninger, P. G. & A. Lucas. 1984. Seasonal variations in condition, reproductive activity, and gross biochemical composition of two species of adult clam reared in a common habitat: Tapes decussates (L.) (Jeffeys) and Tapes philippinarum (Adams & Reeve). J. Exp. Mar. Biol. Ecol. 79:19-37.
Bligh, E. G. & W. F. Dyer. 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37:911-917.
Bourne, N. 1982. Distribution, reproduction, and growth of Manila clam, Tapes philippinarum (Adams & Reeves), in British Columbia British Columbia, province (2001 pop. 3,907,738), 366,255 sq mi (948,600 sq km), including 6,976 sq mi (18,068 sq km) of water surface, W Canada. Geography
. J. Shellfish shellfish, popular name for certain edible mollusks (see Mollusca), e.g., oysters, clams, and scallops, and for certain edible crustaceans, e.g., crabs, lobsters, and shrimps. All are aquatic invertebrates with shells; they are not fish. Res. 2:47-54.
Chung, E. Y., D. K. Ryou & J. H. Lee. 1994. Gonadal development, age and growth of the shortnecked clam, Ruditapes philippinarum (Pelecypoda: Veneridae), on the coast of Kimje, Korea. Korean J. Malucol 10:38-54.
Chung, E. Y., S. B. Hur, Y. B. Hur & J. S. Lee. 2001. Gonadal maturation and artificial spawning of the Manila clam, Ruditapes philippinarum (Pelecypoda: Veneridae), in Komso Bay, Korea. J. Fish. Sci. Tech. 4:208-218.
Couch, C. A. 1989. Carbon and nitrogen stable isotopes of meiobenthos and their food resources. Estuar. Coast. Shelf Sci. 28:433-441.
Dare, P. J. & G. Davies. 1975. Experimental suspended culture of mussels, Mytilus edulis (L), in Wales Wales, Welsh Cymru, western peninsula and political division (principality) of Great Britain (1991 pop. 2,798,200), 8,016 sq mi (20,761 sq km), west of England; politically united with England since 1536. The capital is Cardiff. using spat transplanted from a distant settlement ground. Aquaculture aquaculture, the raising and harvesting of fresh- and saltwater plants and animals. The most economically important form of aquaculture is fish farming, an industry that accounts for an ever increasing share of world fisheries production. 6:257-274.
DeNiro, N. J. & S. Epstein. 1979. Influence of diet on the distribution of carbon isotopes in animals. Geochim. Cosmochim. Acta 42:495-506.
DeNiro, N. J. & S. Epstein. 1981. Influence of diet on the distribution of nitrogen isotopes in animals. Geochim. Cosmochim. Acta 45:341-351.
Dubois, M., K. A. Gilles, J. K. Hamilton, P. A. Rebecs & F. Smith. 1956. Colorimetric col·or·im·e·ter
1. Any of various instruments used to determine or specify colors, as by comparison with spectroscopic or visual standards.
2. method for the determination of sugars and related substances. Anal. Chem. 28:350-356.
Gabbott, P. A. 1975. Storage cycles in marine bivalve mollusks: an hypothesis concerning the relation between glycogen and gametogenesis. In: H. Barnes, editor. Proceedings of the Ninth European Marine Biology marine biology, study of ocean plants and animals and their ecological relationships. Marine organisms may be classified (according to their mode of life) as nektonic, planktonic, or benthic. Nektonic animals are those that swim and migrate freely, e.g. Symposium. Aberdeen, Scotland, UK: Aberdeen University Press. pp. 191-211.
Gabbott, P. A. 1983. Development and seasonal metabolic activities in marine mollusks. In: A. S. M. Saleuddin & K. M. Wilbur, editors. The Mollusca. Vol. 2. New York: Academic Press. pp. 165-217.
Goulletquer, P., R. Robert & G. Trut. 1999. Manila clam Tapes philippinarum culture: sediment-clam interactions. Aquat. Living Resour. 12:45-56.
Holland, D. A. & K. K. Chew. 1974. Reproductive cycle of the Manila clam (Venerupis japonica japonica (jəpŏn`əkə): see quince; camellia. ), from Hood Canal Hood Canal is a fjord off Puget Sound in the U.S. state of Washington Geography
Hood Canal is a fjord off Puget Sound in the U.S. state of Washington with an average width of 1.5 miles (2.4 km) and an average depth of 500 feet (152 m). , Washington. Proc. Nat. Shellfish Ass. 64:53-58.
Holland, D. L. 1978. Lipid reserves and energy metabolism Energy metabolism
Energy metabolism, or bioenergetics, is the study of energy changes that accompany biochemical reactions. Energy sustains the work of biosynthesis of cellular and extracellular components, the transport of ions and organic chemicals against in the larvae Larvae, in Roman religion
Larvae: see lemures. of benthic marine invertebrates. In: D. C. Malins & J. R. Sargent, editors. Biochemical and Biophysical Perspectives in Marine Biology. London: Academic Press. pp. 85-123.
Holm-Hansen, O., C. J. Lorenzen, R. W. Holms & J. D. H. Strickland. 1965. Fluorometric determination of chlorophyll. J. Cons. Perm. Int. Explor. Mer. 30:3-15.
Humason, G. L. 1962. Animal tissue techniques. San Francisco San Francisco (săn frănsĭs`kō), city (1990 pop. 723,959), coextensive with San Francisco co., W Calif., on the tip of a peninsula between the Pacific Ocean and San Francisco Bay, which are connected by the strait known as the Golden : W H Freeman & Co.
Kang, C. K., M. S. Park, P. Y. Lee, W. J. Choi & W. C. Lee. 2000. Seasonal variations in condition, reproductive activity, and biochemical composition of the Pacific oyster Pacific oyster
An oyster (Crassostrea gigas) cultured in the United States and Europe, having a scalloped shell and a fruity flavor. Also called Portuguese oyster. , Crassostera gigas (Thunberg), in suspended culture in two coastal bays of Korea. J. Shellfish Res. 19:771-778.
Kasai, A., H. Horie & W. Sakamoto. 2004. Selection of food sources by Ruditapes philippinarum and Mactra veneriformis (Bivalva: Mollusca) determined from stable isotope analysis. Fish. Sci. 70: 11-20.
Koh, C. H. 2001. The Korean tidal flat: environment, biology and human. Seoul, Korea: Seoul National University Not to be confused with the University of Seoul.
Seoul National University (SNU) is a national research university in Seoul, South Korea. Founded in 1946, SNU was the first national university in South Korea, and served as a model for the many national and public Press. 1073 pp.
Lowry, O. M., N. I. Roseborough, A. L. Farrand & R. J. Randall. 1951. Protein measurement with the folin phenol phenol (fē`nōl), C6H5OH, a colorless, crystalline solid that melts at about 41°C;, boils at 182°C;, and is soluble in ethanol and ether and somewhat soluble in water. reagent reagent /re·a·gent/ (re-a´jent) a substance used to produce a chemical reaction so as to detect, measure, produce, etc., other substances.
n. . J. Biol. Chem. 193:263-275.
Lucas, A. & P. G. Beninger. 1985. The use of physiological condition Noun 1. physiological condition - the condition or state of the body or bodily functions
physical condition, physiological state
wakefulness - a periodic state during which you are conscious and aware of the world; "consciousness during wakefulness in a sane indices in marine bivalve aquaculture. Aquaculture 44:187-200.
Mann, R. & S. J. Glomb. 1978. The effect of temperature on growth and ammonia excretion of the Manila clam Tapes japonica. Est. Coast. Mar. Sci. 6:335-339.
Mann, R. 1979a. Some biochemical and physiological aspects of growth and gametogenesis in Crassostrea gigas and Ostrea eclulis grown at sustained elevated temperature. J. Mar. Biol. Ass. UK. 59:95-100.
Mann, R. 1979b. The effect of temperature on growth, physiology, and gametogenesis in the Manila clam Tapes philippinarum (Adams & Reeve, 1850). J. Exp. Mar. Biol. Ecol. 37:121-133.
Marin, M. G., V. Moschino, M. Deppieri & L. Lucchetta. 2003. Variations in gross biochemical composition, energy value and condition index of T. philippinarum from the Lagoon of Venice. Aquaculture 219:859-871.
Marsh, J. B. & D. B. Weinstein. 1966. Simple charring Charring is a process of incomplete combustion that often occurs when biological tissue (living or dead) is subjected to heat. The resulting matter is sometimes called char. Coke and charcoal are produced this way. method for determination of lipid. J. Lipid Res. 7:57-576.
Melia, P., G. A. De Leo Leo, in astronomy
Leo [Lat.,=the lion], northern constellation lying S of Ursa Major and on the ecliptic (apparent path of the sun through the heavens) between Cancer and Virgo; it is one of the constellations of the zodiac. & M. Gatto. 2004. Density and temperature-dependence of vital rates Vital rates refer to how fast vital statistics change in a population (usually measure per 1000 individuals). There are 2 categories within vital rates: crude rates and refined rates. in the Manila clam Tapes philippinarum: a stochastic By guesswork; by chance; using or containing random values.
stochastic - probabilistic demographic model. Mar. Ecol. Prog. Ser. 272:153-164.
Meneghetti, F., V. Moschino & L. Da Ros. 2004. Gametogenic cycle and variations in oocyte oocyte /oo·cyte/ (-sit) the immature female reproductive cell prior to fertilization; derived from an oogonium. It is a primary o. prior to completion of the first maturation division, and a secondary o. size of Tapes philippinarum from the Lagoon of Venice. Aquaculture 240:473-488.
Minagawa, M. & E. Wada. 1984. Stepwise stepwise
incremental; additional information is added at each step.
stepwise multiple regression
used when a large number of possible explanatory variables are available and there is difficulty interpreting the partial regression enrichment of [sup.15]N along food chains: further evidence and the relation between [delta][sup.15]N and animal age. Geoehim. Cosmochim. Acta 48:1135-1140.
Ministry of Maritime Affairs and Fisheries Republic of Korea. 2005. 2005 Statistical year book of maritime affairs and fisheries, http:// fs.fips.go.kr/index.jsp.
National Fisheries Research and Development Institute. 2004. Korea US Aquaculture. http://www.lib.noaa.gov/korea/main_species/ manila.htm.
Navarro, E., J. I. P. Iglesias & A. Larranaga. 1989. Interannual variation in the reproductive cycle and biochemical composition of the cockle cockle, common name applied to the heart-shaped, jumping or leaping marine bivalve mollusks, belonging to the order Eulamellibranchia. The brittle shells are of uniform size, are obliquely spherical, and possess distinct radiating ridges, or ribs, which aid the Cerastaderma edule from Mundaca Estuary (Biscay, North Spain). Mar. Biol. 101:503-511.
Newell, R. I. & B. L. Bayne. 1980. Seasonal changes in the physiology, reproductive condition and carbohydrate content of the cockle Cardium (= Cerastoderma) edule (Bivalve: Cardiidae). Mar. Biol. 56:11-19.
Ohba, S. 1959. Ecological studies in the natural population of a clam Tapes japonica, with special reference to seasonal variations in the size and structure of the population and to individual growth. Biol. J. Okayama Univ. 5:13-47.
Ojea, J., A. J. Pazos, D. Martinez, S. Novoa, J. L. Sanchez & M. Abad. 2004. Seasonal variation in weight and biochemical composition of the tissues of Ruditapes decussatus in relation to the gametogenic cycle. Aquaculture 238:451-468.
Okumus, I. & H. P. Stirling. 1998. Seasonal variations in the meat weight, condition index and biochemical composition of mussels (Mytilus edulis L.) in suspended culture in two Scottish sea lochs. Aquaculture 159:249-261.
Park, M. S., C. K. Kang & P. Y. Lee. 2001. Reproductive cycle and biochemical composition of the ark shell Scapharca broughtonii (Schrenck) in a southern coastal bay of Korea. J. Shellfish Res. 20:177-184.
Perez Camacho, A., M. Delgado, M. J. Fernandez-Reiriz & U. Labarta. 2003. Energy balance, gonad development and biochemical composition in the clam Ruditales decussatus. Mar. Ecol. Prog. Ser. 258:133-145.
Phillips, D. L. & J. W. Gregg. 2003. Source partitioning using stable isotopes: coping with too many sources. Oecologia 136:261-269.
Ponurovsky, S. K. & Yu. M. Yakovlev. 1992. The reproductive biology of the Japanese littleneck lit·tle·neck
The quahog clam when small and suitable for eating raw. Also called littleneck clam.
[After Little Neck Bay, off western Long Island, New York.]
Noun 1. , Tapes philippinarum (A. Adams & Reeves 1850) (Bivalvia: Veneridae). J. Shellfish Res. 11:265-277.
Riera, P. & P. Richard. 1996. Isotopic determination of food sources of Crassostrea gigas along atrophic atrophic /atro·phic/ (a-tro´fik) pertaining to or characterized by atrophy.
A wasting of cells and tissues. gradient in the estuarine bay of Marennes-Oleron. Estuar. Coast. Shelf Sci. 42:347-360.
Robert, R., G. Trut & J. L. Laborde. 1993. Growth. reproduction and gross biochemical composition of the Manila clam Ruditapes philippinarum in the Bay of Arcachon, France. Mar. Biol. 116:291-299.
Ruiz, C., D. Martinez, G. Mosquera, M. Abad & J. L. Sanchez. 1992. Seasonal variations in condition, reproductive activity and biochemical composition of the flat oyster flat oyster
See European oyster. , Ostrea edulis, from San Cibran (Galicia, Spain). Mar. Biol. 112:67-74.
Sarasquete, M. C., S. Gimeno & M. L. Gonzalez de Canales. 1990. Cycle reproducteur de la palourde Ruditapes philippinarum (Adams & Reeves 1850) de la cote sud ouest atlantique (Espagne). Rev. hit. Oceanogr. Med. 97-98:90-99.
Sbrenna, G. & D. Campioni. 1994. Gametogenic and spawning patterns of Manila clams Tapes philippinarum (Bivalvia: Veneroida) in two lagoons of the River Po Delta, Italy. J. Shellfish Res. 13:37-46.
Tanaka, Y. 1954. Spawning season of important bivalves in Ariake Bay III Venerupis semidecussata (Reeve). Bull. Jap. Soc. Sci. Fish. 19:1165-1167.
Walne, P. R. 1970. The seasonal variation of meat and glycogen content of seven populations of oysters Ostrea edulis L. and a review of literature. Fishery Invest. Lond. Ser. 2:35.
Xie, Q. & G. M. Burnell. 1994. A comparative study of the gametogenic cycles of the clams Tapes philippinarum (A. Adams & Reeve 1850) and Tapes decussates (Linnaeus) on the south coast of Ireland. J. Shellfish Res. 13:467472.
Zandee, D. I., J. H. Kluytmans, W. Zurburg & H. Pieters. 1980. Seasonal variations in biochemical composition of Mytilus edulis with reference to energy metabolism and gametogenesis. Neth. J. Sea Res. 14:1-29.
CHANG-KEUN KANG, (1) * YANG SOON KANG, (2) EUN EUN Egyptian Universities Network
EUN Laayoune, Morocco - Laayoune-Hassan I Morocco (Airport Code)
EUN Endogenous Urinary Nitrogen
EUN External Update Notification
EUN End User Network JUNG CHOY, (1) DONG-SUN KIM, (3) BONG-TAEK SHIM (4) AND PIL-YONG LEE (2)
(1) Department of Biology, Pusan National University, Busan 609-735, Republic of Korea; (2) Department of Oceanography oceanography, study of the seas and oceans. The major divisions of oceanography include the geological study of the ocean floor (see plate tectonics) and features; physical oceanography, which is concerned with the physical attributes of the ocean water, such as , National Fisheries Research and Development Institute, Busan 619-900, Republic of Korea; (3) Research Center for Ocean Industrial Development (RCOID), Pukyong National University Pukyong National University is one of the 10 large-scale national universities in Busan, South Korea. University has two campuses - Daeyeon-dong and Yongdang-dong. These campuses are situated near coastal district of Nam-gu. , 608-737 Busan, Republic of Korea; (4) Goseong Maritime and Fisheries Office, Masan Regional Maritime Affairs & Fisheries Office. 638-805 Gyeongnam, Republic of Korea
* Corresponding author. E-mail: firstname.lastname@example.org
TABLE 1. Sediment characteristics of the experimental sites, and initial density, shell length, and final density of the clam. Data are from May 6 2000, when the sites were seeded with clams, except for final density (October 30 2001). Data are means (ranges in parentheses). Organic % Mud Median Grain Content Site <63 [micro]m Size ([micro]m) (% Ignition loss) Nonsanded mudflat 83 23 7.5 (7.0-8.2) Created habitat 17 193 2.5 (1.0-7.7) Natural habitat 20 185 2.6 (1.0-8.0) Initial (seed) Density Final Density (individuals Initial Shell (Individuals Site [m.sup.-2]) Length (mm) [m.sup.-2]) Nonsanded mudflat 153 (138-164) 25.3 (18.0-33.9) 0 Created habitat 112 (100-131) 24.8 (17.6-41.2) 34 (22-39) Natural habitat 100 (92-123) 24.5 (20.0-38.1) 23 (20-28) TABLE 2. [[delta].sup.13]C and [[delta].sup.15]N values (mean [+ or -] SD) for microphytobenthos, marine POM (particulate organic matter), riverine POM, and clams collected in Jinju Bay. Figures in parentheses are the number of analyzed samples. August Sample 2000 [[delta].sup.13]C Microphytobenthos -13.7 [+ or -] 1.6 (2) Marine POM -22.0 [+ or -] 1.3 (2) Riverine POM -28.2 [+ or -] 1.1 (2) Clams (control) -17.9 [+ or -] 0.2 (4) Clams (treatment) -17.8 [+ or -] 0.5 (4) [[delta].sup.15]N Microphytobenthos 8.9 [+ or -] 1.6 (2) Marine POM 4.6 [+ or -] 1.6 (2) Riverine POM 9.5 [+ or -] 0.6 (2) Clams (control) 11.3 [+ or -] 0.3 (4) Clams (treatment) 11.4 [+ or -] 0.5 (4) Sample October [[delta].sup.13]C Microphytobenthos -13.8 [+ or -] 0.5 (2) Marine POM -21.9 [+ or -] 0.8 (2) Riverine POM -30.4 [+ or -] 2.1 (2) Clams (control) -17.8 [+ or -] 0.9 (5) Clams (treatment) -17.8 [+ or -] 0.3 (4) [[delta].sup.15]N Microphytobenthos 9.7 [+ or -] 0.1 (2) Marine POM 5.6 [+ or -] 0.5 (2) Riverine POM 10.0 [+ or -] 0.9 (2) Clams (control) 11.3 [+ or -] 0.6 (4) Clams (treatment) 11.2 [+ or -] 0.3 (4) Sample December [[delta].sup.13]C Microphytobenthos -13.9 [+ or -] 0.1 (2) Marine POM -20.7 [+ or -] 0.9 (2) Riverine POM -31.3 [+ or -] 0.3 (2) Clams (control) -16.9 [+ or -] 0.8 (4) Clams (treatment) -17.9 [+ or -] 0.3 (4) [[delta].sup.15]N Microphytobenthos 8.9 [+ or -] 0.7 (2) Marine POM 5.6 [+ or -] 1.0 (2) Riverine POM 8.3 [+ or -] 0.2 (2) Clams (control) 11.0 [+ or -] 0.7 (4) Clams (treatment) 11.0 [+ or -] 0.2 (4) February Sample 2001 [[delta].sup.13]C Microphytobenthos -14.9 [+ or -] 0.8 (2) Marine POM -20.2 [+ or -] 0.9 (2) Riverine POM -26.4 [+ or -] 1.1 (2) Clams (control) -17.3 [+ or -] 0.3 (5) Clams (treatment) -17.0 [+ or -] 0.7 (5) [[delta].sup.15]N Microphytobenthos 9.7 [+ or -] 0.8 (2) Marine POM 7.0 [+ or -] 0.4 (2) Riverine POM 8.3 [+ or -] 1.0 (2) Clams (control) 11.0 [+ or -] 0.4 (4) Clams (treatment) 10.1 [+ or -] 0.6 (4) Sample April [[delta].sup.13]C Microphytobenthos -17.4 [+ or -] 0.6 (2) Marine POM -20.7 [+ or -] 1.5 (2) Riverine POM -24.4 [+ or -] 0.2 (2) Clams (control) -17.2 [+ or -] 0.3 (4) Clams (treatment) -17.5 [+ or -] 0.2 (4) [[delta].sup.15]N Microphytobenthos 7.6 [+ or -] 0.7 (2) Marine POM 6.7 [+ or -] 0.1 (2) Riverine POM 8.6 [+ or -] 0.3 (2) Clams (control) 10.7 [+ or -] 0.4 (3) Clams (treatment) 10.3 [+ or -] 0.4 (4) Sample June [[delta].sup.13]C Microphytobenthos -13.2 [+ or -] 1.1 (2) Marine POM -20.8 [+ or -] 0.2 (2) Riverine POM -26.0 [+ or -] 0.1 (2) Clams (control) -17.4 [+ or -] 0.7 (4) Clams (treatment) -18.0 [+ or -] 0.6 (4) [[delta].sup.15]N Microphytobenthos 8.4 [+ or -] 0.5 (2) Marine POM 3.7 [+ or -] 0.1 (2) Riverine POM 9.1 [+ or -] 0.2 (2) Clams (control) 9.2 [+ or -] 0.7 (4) Clams (treatment) 9.3 [+ or -] 0.3 (3) Sample Total [[delta].sup.13]C Microphytobenthos -14.5 [+ or -] 1.6 (12) Marine POM -21.1 [+ or -] 1.1 (12) Riverine POM -27.8 [+ or -] 2.7 (12) Clams (control) -17.4 [+ or -] 0.6 (26) Clams (treatment) -17.6 [+ or -] 0.6 (25) [[delta].sup.15]N Microphytobenthos 8.9 [+ or -] 0.9 (12) Marine POM 5.7 [+ or -] 1.3 (12) Riverine POM 9.0 [+ or -] 0.8 (12) Clams (control) 10.8 [+ or -] 0.8 (23) Clams (treatment) 10.5 [+ or -] 0.8 (23)