Clostridium perfringens. (Junior Division).The purpose of this research was to determine the presence of Clostridium perfringens found in or on dry soil, water, silage silage (sī`lĭj) or ensilage (ĕn`səlĭj), succulent, moist feed made by storing a green crop in a silo. The crop most used for silage is corn; others are sorghum, sunflowers, legumes, and grass. , soil in a barn, cow bedding, and cow udders. C. perfringens is a soil-borne Gram positive bacteria, is hemolytic he·mo·lyt·ic adj. Destructive to red blood cells; hematolytic. Hemolytic Referring to the destruction of the cell membranes of red blood cells, resulting in the release of hemoglobin from the damaged cell. , and produces the enzyme lecithinase lec·i·thin·ase n. See phospholipase. lecithinase an enzyme that splits lecithin. Called also phospholipase. , and is anaerobic anaerobic /an·aer·o·bic/ (an?ah-ro´bik) 1. lacking molecular oxygen. 2. growing, living, or occurring in the absence of molecular oxygen; pertaining to an anaerobe. . Soil and water samples were obtained, diluted with tap water, smeared onto blood agar plates, then cultured in anaerobic conditions. Anaerobic conditions were achieved using GasPac pouches clinically used for anaerobic culturing. Using an inoculating loop, the hemolytic colonies were isolated then streaked onto egg yolk yolk (yok) the stored nutrient of an oocyte or ovum. yolk n. The portion of the egg of an animal that consists of protein and fat from which the early embryo gets its main nourishment and of plates for isolation of bacteria producing the lecithinase enzyme. It was concluded that Clostridium perfringens is present and a dominant bacteria in dirty livestock bedding, manure saturated soil, and on cow udders. This poses a risk to young and weak livestock of developing enterotoxaemia, caused by ingesting the bactera. Kori Tagtmeyer, Hi-Plains High School. |
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