Cloning and expression of recombinant membrane receptor 1 in SF9 insect cells.A recombinant form of Adiponectin Membrane Receptor 1 will be produced for transfection trans·fec·tion n. Infection of a bacterium or cell with DNA or RNA isolated from a bacteriophage or from an animal or a plant virus, resulting in replication of the complete virus. into Sf9 insect cells. This receptor binds to adiponectin, which is a hormone released from adipose tissue adipose tissue (ăd`əpōs'): see connective tissue. adipose tissue or fatty tissue Connective tissue consisting mainly of fat cells, specialized to synthesize and contain large globules of fat, within a . Adiponectin is a protein with insulin-sensitizing properties, which is an important fact for individuals plagued with diabetes and obesity. Research has shown that it is attributed to enhancing the uptake of glucose into skeletal muscle. The DNA DNA: see nucleic acid. DNA or deoxyribonucleic acid One of two types of nucleic acid (the other is RNA); a complex organic compound found in all living cells and many viruses. It is the chemical substance of genes. of the receptor, AdipoR1, will be cloned into a PiEX-4 vector, then transfected into SF-9 cells. Once expression in the insect cells occurs, structural analysis will be performed to determine cell signaling and binding mechanisms. Further understanding of the sub-cellular mechanisms of this receptor could aid in the discovery of inhibitory therapies that would increase insulin sensitivity insulin sensitivity The systemic responsiveness to glucose, which can be measured by 1. The insulin sensitivity index–measures the ability of endogenous insulin to ↓ glucose in extracellular fluids by inhibiting glucose release from the liver and , which would prove a remarkable breakthrough for obese and diabetic individuals. |
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