Canadian Forest Service obtains United States patent.Canadian Forest Service The Canadian Forest Service (CFS) is a sector of the Canadian government department of Natural Resources Canada. Part of the federal government since 1899, the CFS is a science-based policy organization responsible for promoting the sustainable development of Canada's forests and (Canada) has patented an isolated polynucleotide polynucleotide /poly·nu·cleo·tide/ (-noo´kle-o-tid) any polymer of mononucleotides. pol·y·nu·cle·o·tide n. sequence useful as a promoter in plant root tissue. The sequence is derived from the promoter for the Western white pine gene PR10. The sequence comprises at least 45 nucleotidcs, and has at least two Dof elements which individually may be in a forward or inverted inverted reverse in position, direction or order. inverted L block a pattern of local filtration anesthesia commonly used in laparotomy in the ox. orientation. Each Dof element has a sequence NNNWAAAGNNN, wherein where·in adv. In what way; how: Wherein have we sinned? conj. 1. In which location; where: the country wherein those people live. 2. N is A, T, G, or, C, and, W is A or T. The Dof elements are separated from each other by between 10 and 20 nucleotides. The sequence also has a TATA box TATA box a eukaryotic DNA sequence usually TATAAATA, similar to the Pribnow box of Escherichia coli, occurring in the promoter region 25 to 35 bases upstream from the transcriptional start site that binds the general transcription factor TFIID which begins the formation of located between 15 and 25 nucleotides in the 3' direction of the closest Dof element. In some instances, the polynucleotide has one Dof element with the sequence NNNWAAAGNNN and another with the sequence NNNCTTTWNNN. In some instances the polynucleotide sequence is one where the 5' Dof element sequence is CTCTCTTTAAT, and another Dof element (which may be the next Dof element) sequence is TTTAAAGGTT and the TATA box sequence is TACAATAAATA or TATAWAWA, wherein W is A or T. Also provided are sequences useful in modulating gene expression in stem and leaf tissue. (US 7,285,656) |
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