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Baylisascaris procyonis: an emerging helminthic zoonosis. (Synopsis).

Baylisascaris procyonis, a roundworm infection of raccoons, is emerging as an important helminthic zoonosis, principally affecting young children. Raccoons have increasingly become peridomestic animals living in close proximity to human residences. When B. procyonis eggs are ingested by a host other than a raccoon, migration of larvae through tissue, termed larval migrans, ensues. This larval infection can invade the brain and eye, causing severe disease and death. The prevalence of B. procyonis infection in raccoons is often high, and infected animals can shed enormous numbers of eggs in their feces. These eggs can survive in the environment for extended periods of time, and the infectious dose of B. procyonis is relatively low. Therefore, the risk for human exposure and infection may be greater than is currently recognized.

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Baylisascaris procyonis, a ubiquitous roundworm infection of raccoons (Procyon lotor), is increasingly being recognized as a cause of severe human disease (1,2). B. procyonis has a widespread geographic distribution, with infection rates as high as 70% in adult raccoons and exceeding 90% in juvenile raccoons (3). As with other ascarids, eggs are excreted in feces and must develop externally, typically in soil, to become infectious. When raccoons ingest infective eggs, larvae will hatch, enter the wall of the small intestine, and subsequently develop to adult worms in the small bowel. However, ingestion of eggs by other host animals, especially rodents and other small mammals, results in extraintestinal migration of larvae (4); an estimated 5%-7% of larvae invade the brain (5). The migration of helminth larvae through tissue in suboptimal hosts is termed larva migrans and may affect the viscera (visceral larva migrans [VLM]), the eye (ocular larva migrans [OLM]), or the nervous system (neural larva migrans [NLM] (6). Raccoons :may also become infected when they eat larvae that have become encapsulated in the tissues of rodents and other animals (3).

More than 90 species of wild and domesticated animals have been identified as infected with B. procyonis larvae (3). Outbreaks of fatal central nervous system disease caused by B. procyonis have occurred on farms and in zoos and research animal colonies and have affected commercial chickens, bob-white quail, guinea pigs, commercial pheasants, and domestic rabbits (7-11). Natural infections have also been recognized in dogs, rodents, porcupines, chinchillas, prairie dogs, primates, woodchucks, emus, foxes, and weasels (12-16). Experimental infection of a variety of nonhuman primates has also been reported (17).

Human Infection

B. procyonis infection of humans typically results in fatal disease or severe sequelae (1,2,18-24; pers. comm., W. Murray). Clinical manifestations include eosinophilic encephalitis, ocular disease, and esoinophilic cardiac pseudotumor. Elevated peripheral cerebrospinal fluid eosinophilia can be detected in cases of meningoencephalitis. Eleven recognized human cases, four of them fatal, have been reported (Table). The first human case was reported in 1984 in a 10-month-old infant with fatal eosinophilic meningoencephalitis (18). At autopsy, numerous granulomas containing larvae of B. procyonis were observed in several organs and tissues (18). The brain was the most heavily affected, with granulomas concentrated in the periventricular white matter, around the dentate nuclei, and along the cerebral and cerebellar cortices. Numerous granulomas and larvae were also found in the mesentery and cardiac tissue. The infant's family lived in a rural, wooded area of Pennsylvania, and raccoons were nesting in unused chimneys at the time infection was acquired.

Four additional cases of eosinophilic encephalitis with similar pathologic characteristics have been documented. Magnetic resonance images from a human case of Baylisascaris encephalitis are shown in Figure 1. In patients who have survived central nervous system (CNS) invasion, severe neurologic sequelae have resulted. In a fatal case, an eosinophilic cardiac pseudotumor, affecting principally the left ventricle, was observed at autopsy; no larvae or granulomas were found in any other tissue examined.

[FIGURE 1 OMITTED]

No effective therapy exists for the visceral form of B. procyonis larval infection. In an experimental model, mice treated with albendazole and diethylcarbamezine within 10 days after infection were protected from CNS disease (25); however, several anthelminthic agents have been used to treat human cases without success. Laser photocoagulation has been successful in treating ocular infection (26).

Because the disease is transmitted by the fecal-oral route, human cases of B. procyonis infection typically occur in younger age groups, mainly infants, who often engage in oral exploration of their environment and are therefore more likely to be exposed to B. procyonis eggs. Raccoon activity near the patient's residence is often described. All but one of the reported patients to date have been male, however; there is no reason to believe that females are less susceptible to infection.

Diagnosis and Underrecognition of Infection

Diagnosis of B. procyonis infection is typically done through morphologic identification of larvae in tissue sections (27). However, accurate diagnosis requires experience in recognizing larval morphologic characteristics and differentiating among a number of possible larval nematode agents, including Toxocara canis, T. cati, Ascaris lumbricoides, and species of Gnathastoma, Angiostrongylus, and Ancylostoma, as well as larval cestode infections such as cysticercosis and echinococcosis (6,27). Characteristic features of B. procyonis larvae in tissue include its relatively large size (60 [mu]) and prominent single lateral alae (27) (Figure 2). While serologic testing has been performed in some cases as supportive diagnostic evidence, no commercial serologic test is currently available (28,29). However, a presumptive diagnosis can be made on the basis of clinical (meningoencephalitis, diffuse unilateral subacute neuroretinitis [DUSN], pseudotumor), epidemiologic (raccoon exposure), radiologic (white matter disease), and laboratory results (blood and CNS eosinophilia).

[FIGURE OMITTED]

Human baylisascariasis is probably underrecognized, and the full spectrum of clinical illness is unclear. The agent is unknown to most clinicians and typically is not considered in a differential diagnosis. In addition, confirming the diagnosis requires an effective biopsy specimen that must contain an adequate cross-section of a larva. Since small numbers of larvae can cause severe disease and larvae occur sporadically in tissue, a biopsy may frequently fail to include larvae; such a specimen will result in a negative finding. Moreover, larval morphologic characteristics may not be recognized or may be misidentified. The accurate diagnosis of parasites in tissues can be difficult even for trained microscopists, and mistaken identification, particularly of helminth larvae, is not uncommon (27). Finally, no commercial serologic test exists for the diagnosis of B. procyonis infection, and the sensitivity, specificity, and predictive value of available serologic tests are unknown. Evidence for underrecognition of larval B. procyonis infection can be found in several reported cases of DUSN caused by larvae compatible with B. procyonis and a case of eosinophilic meningoencephalitis reported in an infant in 1975 (26,30,31).

Infection Potential and Human Risk

Although relatively few human cases of baylisascariasis have been reported, several factors suggest that the likelihood of exposure and infection may be greater than is currently recognized. Raccoons have a widespread geographic distribution, and infection with B. procyonis is common in raccoon populations, with typically high prevalence rates observed. An infected raccoon can harbor numerous adult worms and may excrete large numbers of eggs. A single adult female worm may produce an estimated 115,000 to 877,000 eggs per day, and an infected raccoon can shed as many as 45,000,000 eggs daily (3,4,32). In light of the relatively low infectious dose of B. procyonis (estimated to be [less than or equal to] 55,000 eggs) and the viability of the eggs in the environment for months to years, the infection potential is not insubstantial. Raccoons have increasingly become peridomestic animals living in close proximity to human residences and are among the fastest growing wildlife populations nationwide. These animals benefit from feeding on abundant pet food left accessible, either accidentally or intentionally, and their populations can thrive under such conditions. In one suburban area near the residence of a recent patient in northern California, the raccoon population was measured at 30 animals per quarter acre. Areas frequented by raccoons and used for defecation were found in close proximity to human dwellings, and B. procyonis eggs are routinely recovered from these areas (1). Children, particularly toddlers, may be at particular risk of exposure.

Although baylisascariasis may indeed be underdiagnosed, asymptomatic human infection may be the typical response, and the limited number of cases reported may indicate that an unrecognized immune defect is necessary for severe infection to occur. The prevalence of asymptomatic infection in human populations has yet to be determined.

A Possible Agent of Bioterrorism

In an era of increasing concern about bioterrorism (33), certain characteristics of B. procyonis make it a feasible bioterrorist agent. The organism is ubiquitous in raccoon populations and therefore easy to acquire. Enormous numbers of eggs can be readily obtained, and these eggs can survive in an infectious form for prolonged periods of time. As with other ascarids, the eggs can remain viable in a dilute (0.5%-2%) formalin solution for all indefinite period of time, and animal studies suggest that B. procyonis has a relatively small infectious dose. Moreover, the organism causes a severe, frequently fatal infection in humans, and no effective therapy or vaccine exists. Introduction of sufficient quantities or B. procyonis eggs into a water system or selected food products could potentially result in outbreaks of the infection. A similar agent, Ascaris suum, a roundworm of pigs, was used to intentionally infect four university students who required hospitalization after eating a meal that had been deliberately contaminated with a massive dose of eggs (34). Contamination of community water sources would be difficult since the eggs of B. procyonis are relatively large (80 [micro]m long by 65 [micro]m wide) and would be readily removed by standard filtration methods or the flocculation and sedimentation techniques used by municipal water systems in the United States. However, posttreatment contamination or targeting of smaller systems could be possible.

Conclusion

Baylisascariasis is an emerging helminthic zoonosis with the potential for severe infection that may be a more important public health problem than is currently recognized. Educating the medical community is of paramount importance in helping to define the extent of infection. Physicians should consider B. procyonis infection in the differential diagnosis of patients with eosinophilic meningoencephalitis, DUSN, and eosinophilic pseudotumor. While infants and children have a higher probability of infection, all age groups are at risk. The public should be made aware of the potential risks of exposure to raccoons and raccoon feces. Raccoons should be discouraged as pets or should be routinely evaluated for B. procyonis infection and treated. However, screening and treatment may not be sufficient to prevent exposure, since the likelihood of reinfection is high. The public should be discouraged from feeding raccoons and should ensure that possible food sources (such as pet food, water, and garbage) are protected from raccoon access. Further study of the impact of larval B. procyonis infection on human health is warranted. Development of a standardized serologic test for B. procyonis would allow epidemiologic studies of its prevalence and incidence and help determine factors associated with infection. A sensitive and specific test would also provide a noninvasive method of diagnosis. Finally, a better understanding of the pathogenesis of B. procyonis infection and efforts to develop effective treatment approaches are warranted.
Table. Reported human cases of larval Baylisascaris procyonis infection

Year (a) Location Age Sex Clinical

1980 Pennsylvania 10 mo Male Eosinophilic
 meningoencephalitis
1984 Illinois 18 mo Male Eosinophilic
 meningoencephalitis
1990 New York 13 mo Male Eosinophilic
 meningoencephalitis
1992 California 29 yr Male Diffuse unilateral subacute
 neuroretinitis
1991 Germany 48 yr Female Diffuse unilateral subacute
 neuroretinitis
1995 Massachusetts 10 yr Male Esoinophilic cardiac
 pseudotumor
1996 Michigan 6 yr Male Chorioretinitis, neurologic
 deficits
1996 Michigan 2 yr Male Eosinophilic
 meningoencephalitis,
 chorioretinitis
1997 California 13 mo Male Eosinophilic
 meningoencephalitis
1998 California 11 mo Male Eosinophilic encephalitis
1999 California 17 yr Male Eosinophilic
 meningoencephalitis

Year (a) Location Outcome Reference

1980 Pennsylvania Fatal 17
1984 Illinois Fatal 18
1990 New York Severe neurologic 19
 sequelae
1992 California Ocular sequelae 21
1991 Germany Ocular sequelae 22
1995 Massachusetts Fatal 20
1996 Michigan Severe neurologic 23
 sequelae
1996 Michigan Severe neurologic 23
 sequelae
1997 California Severe neurologic 2
 sequelae
1998 California Severe neurologic 1
 sequelae
1999 California Fatal (b)

(a) year of onset or report.

(b) Pers. comm., W. Murray.


Acknowledgment

The authors thank Howard A. Rowley for graciously providing the images in Figure 1.

Dr. Sorvillo is Associate Professor, Department of Epidemiology, UCLA School of Public Health. His research interests include the epidemiology and control of infectious diseases, particularly parasitic agents.

References

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(2.) Rowley HA, Uht RM, Kazacos KR, Sakanari J, Wheaton WV, Barkovich AJ, et al. Radiologic-pathologic findings in raccoon roundworm (Baylisascaris procyonis) encephalitis. Am J Neuroradiol 2000;21:415-20.

(3.) Kazacos KR, Boyce WM. Baylisascaris larva migrans. J Am Vet Med Assoc 1989;195:894-903.

(4.) Hamann KJ, Kephart GM, Kazacos KR, Gleich GJ. Immunofluorescent localization of eosinophil granule major basic protein in fatal human cases of Baylisascaris procyonis infection. Am J Trop Med Hyg 1989;40:291-7.

(5.) Kazacos KR. Raccoon ascarids as a cause of larva migrans. Parasitol Today 1986;2:253-5.

(6.) Beaver P, Jung RC, Cupp EW. Clinical parasitology. Philadelphia: Lea & Febiger; 1984.

(7.) Richardson JA, Kazacos KR, Thacker HL, Dhillon AS, Winterfield RW. Verminous encephalitis in commercial chickens. Avian Dis 1979;24:498-503.

(8.) Kazacos KR, Kazacos EA, Render JA, Thacker HL. Cerebrospinal nematodiasis and visceral larva migrans in an Australian (Latham's) brush turkey. J Am Vet Med Assoc 1982;818:1295-8.

(9.) Kazacos KR, Reed WM, Thacker HL. Cerebrospinal nematodiasis in pheasants. J Am Vet Med Assoc 1986;189:1353-4.

(10.) Van Andel RA, Franklin CL, Besch-Williford C, Riley LK, Hook RR, Kazacos KR. Cerebrospinal larva migrans due to Baylisascaris procyonis in a guinea pig colony. Lab Anim Science 1995;45:27-30.

(11.) Kazacos KR, Reed WM, Kazacos EA, Thacker HL. Fatal cerebrospinal disease caused by Baylisascaris procyonis in domestic rabbits. J Am Vet Med Assoc 1983;183:967-71.

(12.) Kazacos KR, Appel GO, Thacker HL. Cerebrospinal nematodiasis in a woodchuck suspected of having rabies. J Am Vet Med Assoc 1981;179:1102-4.

(13.) Campbell GA, Hoover JP, Russell WC, Breazile JE. Naturally occurring cerebral nematodiasis due to Baylisascaris larval migration in two black-and-white ruffed lemurs (Varecia variegata variegata) and suspected cases in three emus (Dromaius novaehollandiae). J Zoo Wildl Med 1997;8:204-7.

(14.) Williams CK, McKown RD, Veatch JK, Applegate RD. Baylisascaris sp. found in a wild northern bobwhite (Colinus virginianus). J Wildl Dis 1997;33:158-60.

(15.) Rudmann DG, Kazacos KR, Storandt ST, Harris DL, Janovitz EB. Baylisascaris procyonis larva migrans in a puppy: a case report and update for the veterinarian. J Am Anim Hosp Assoc 1996;32:73-6.

(16.) Fitzgerald SD, White MR, Kazacos KR. Encephalitis in two porcupines due to Baylisascaris larval migration. J Vet Diagn Invest 1991;3:359-62.

(17.) Ball RL, Dryden M, Wilson S, Veatch J. Cerebrospinal nematodiasis in a white-handed gibbon (Hylobates lar) due to Baylisascaris sp. J Zoo Wildl Med 1998;29:221-4.

(18.) Huff DS, Neafie RC, Binder MJ, De Leon GA, Brown LW, Kazacos KR. The first fatal Baylisascaris infection in humans: an infant with eosinophilic meningoencephalitis. Pediatr Pathol 1984;2:345-52.

(19.) Fox AS, Kazacos KR, Gould FT, Thomas C, Boyer KM. Fatal eosinophilic meningoencephalitis and visceral larva migrans caused by the raccoon ascarid Baylisascaris procyonis. N Engl J Med 1985;312:1619-23.

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(22.) Goldberg MA, Kazacos KR, Boyce WM, Ai E, Katz B. Diffuse unilateral subacute neuroretinitis. Morphometric, serologic, and epidemiologic support for Baylisascaris as a causative agent. Ophthalmology 1993;100:1695-701.

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(24.) Gavin PJ, Shulman ST, Kazacos KR, Davis AT, Mets MB, Byrd S, et al. Neural larva migrans caused by the raccoon roundworm Baylisascaris procyonis: Two pediatric cases from Chicago, Illinois. American Society of Microbiology Conference, May 2001, Abstract No. C-443.

(25.) Kazacos KR. Byalisascaris procyonis and related species. In: Sanuel WM, Pybus MJ, Cawthorn RJ, editors. Parasitic diseases of wild mammals. Ames (IA): Iowa State University Press; 2001.

(26.) Raymond LA, Gutierrez Y, Strong LE, Wander AH, Buten R, Cordan D. Living retinal nematode (filarial-like) destroyed with photocoagulation. Ophthamology 1978;85:944-9.

(27.) Orihel T, Ash LR. Parasites in human tissue. Chicago: American Society of Clinical Pathologists; 1995.

(28.) Boyce WM, Asai DJ, Wilder JK, Kazacos KR. Physicochemical characterization and monoclonal and polyclonal antibody recognition of Baylisascaris procyonis larval excretory-secretory antigens. J Parasitol 1989;75:540-8.

(29.) Boyce WM, Branstetter BA, Kazacos KR. Comparative analysis of larval excretory-secretory antigens of Baylisascaris procyonis, Toxocara canis and Ascaris suum by Western blotting and enzyme immunoassay. Int J Parasitol 1988;18:109-13.

(30.) Gass JDM, Gilbert WR, Guerry RK, Scelfo R. Diffuse unilateral subacute neuroretinitis. Opthalmology 1978:85:521-45.

(31.) Anderson DC, Greenwood R, Fishman M, Kagan IG. Acute infantile hemiplegia with cerebrospinal fluid eosinophilic pleocytosis: an unusual case of visceral larva migrans. J Pediatr 1975;86:247-9.

(32.) Jacobson JE, Kazacos KR, Montague FH. Prevalence of eggs of Baylisascaris procyonis (Nematoda:Ascaroidea) in raccoon scats from an urban and a rural community. J Wildl Dis 1982;18:461-4.

(33.) Henderson DA. The looming threat of bioterrism. Science 1999;283:1279-82.

(34.) Phills JA, Harrold AJ, Whiteman GV, Perelmutter L. Pulmonary infiltrates, asthma and eosinophilia due to Ascaris suum infestation in man. N Engl J Med 1972;286:965-70.

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Address for correspondence: Frank Sorvillo, UCLA School of Public Health, Box 951772, Los Angeles, CA 90024, USA; fax: 714-816-9099; e-mail: fsorvill@ucla.edu

Frank Sorvillo, * Lawrence R. Ash, * O.G.W. Berlin, * ([dagger]) JoAnne Yatabe, ([dagger]) Chris Degiorgio, ([double dagger]) and Stephen A. Morse ([section])

* University of California Los Angeles School of Public Health, Los Angeles, California; ([dagger]) Specialty Labs, Santa Monica, California, USA; ([double dagger]) University of California Los Angeles School of Medicine, Los Angeles, California, USA; and ([section]) Centers for Disease Control and Prevention, Atlanta Georgia, USA
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Author:Morse, Stephen A.
Publication:Emerging Infectious Diseases
Geographic Code:1USA
Date:Apr 1, 2002
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