Bacteriophages; methods and protocols; v.1: Isolation, characterization, and interactions.9781588296825 Bacteriophages; methods and protocols; v.1: Isolation, characterization, and interactions. Ed. by Martha R. J. Clokie and Andrew M. Kropinski. Humana Press Inc. 2009 307 pages $119.00 Hardcover Methods in molecular biology; 501 QR342 Clokie (U. of Leicester, UK) and Kropinski (Public Health Agency of Canada, Ontario) present a two-volume work on bacteriophages methods and protocols for experienced and new bacteriophage researchers; each volume is sold separately and has a different ISBN. The first volume features 25 chapters by 43 international academics and researchers focusing on the isolation of phages from a range of environments, their morphological and molecular characterization, and methods for the investigation of their interaction with bacteria. The second volume addresses bacteriophage genomics, metagenomics, transcriptomics, proteomics, and applied bacteriophage biology. A sampling of topics from the first volume: bacteriophage enrichment from water and soil, isolation of phage via induction of lysogens, enumeration of bacteriophages by double agar overlay plaque assay, basic phage electron microscopy, construction of phage mutants, generalized transduction, and preparation and characterization of anti-phage serum. ([c]2009 Book News, Inc., Portland, OR) |
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