Avian Pathol: An experimental model to quantify horizontal transmission of Mycoplasma gallisepticum.Before interventions to control horizontal transmission horizontal transmission n. Transmission of infection by contact. horizontal transmission Epidemiology The transmission of an infection from one to another person of the same generation in the same population. of Mycoplasma mycoplasma Any of the bacteria that make up the genus Mycoplasma. They are among the smallest of bacterial organisms. The cell varies from a spherical or pear shape to that of a slender branched filament. gallisepticum can be tested, a suitable experimental model should be available. Transmission dynamics in a flock can be quantified by 2 parameters: the average number of secondary cases infected by one typical infectious case (RO) and the number of new infections that occur due to one infectious animal per unit of time ([beta]). The transmission dynamics of M gallisepticum have not been studied experimentally, so the aim of this study was to examine the horizontal transmission of M gallisepticum. The study was carried out using a pairwise design with 3 different inoculation doses. Every pair consisted of an inoculated chicken and a susceptible in-contact chicken. Five susceptible individually housed chickens were placed in between pairs to measure airborne transmission. Infection was detected by serology Serology The division of biological science concerned with antigen-antibody reactions in serum. It properly encompasses any of these reactions, but is often used in a limited sense to denote laboratory diagnostic tests, especially for syphilis. , quantitative polymerase chain reaction Quantitative polymerase chain reaction (qPCR) is a modification of the polymerase chain reaction used to rapidly measure the quantity of DNA, complementary DNA or ribonucleic acid present in a sample. , and culture. The inoculated and in-contact chickens were equally infectious and the pairs could be regarded as independent. The RO was estimated to be greater than 1 (95% confidence interval), the estimated [beta] was 0.22 per day, and there was no significant difference between the different inoculation doses. It was concluded that the animal model as described in this study meets the conditions for the establishment of transmission dynamics of M gallisepticum and, therefore, can be used to establish the quantitative effect of intervention measures on horizontal transmission. 2005;34:355-361. |
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