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Analysis of anti-HIV-1 Tat hammerhead ribozyme catalytic activity. (Withdrawn).

The Human Immunodeficiency Virus human immunodeficiency virus

Human immunodeficiency virus (HIV)
A transmissible retrovirus that causes AIDS in humans.
 (HIV-1) is a retrovirus retrovirus, type of RNA virus that, unlike other RNA viruses, reproduces by transcribing itself into DNA. An enzyme called reverse transcriptase allows a retrovirus's RNA to act as the template for this RNA-to-DNA transcription.  that causes the Acquired Immune Deficiency Syndrome Acquired immune deficiency syndrome (AIDS)

A viral disease of humans caused by the human immunodeficiency virus (HIV), which attacks and compromises the body's immune system.
 (AIDS). HIV-1 infects CD4+ cells, including T helper lymphocytes, the destruction of which renders the immune system and human body defenseless. AIDS treatments include drug and potential gene therapies. However, gene therapies are potentially curative, whereas drug therapies act by preventing infection of additional cells. In the area of gene therapy, ribozymes have been of particular interest. Ribozymes are small catalytic RNAs that specifically cleave single-stranded RNA RNA: see nucleic acid.
 in full ribonucleic acid

One of the two main types of nucleic acid (the other being DNA), which functions in cellular protein synthesis in all living cells and replaces DNA as the carrier of genetic
 targets. Our research includes the use of specifically designed anti-HIV-1 hammerhead ribozymes. These ribozymes are designed to cleave Tat mRNA from the HIV-1 strain NL43. Tat is a small virally encoded protein that is responsible for regulation of viral transcription. Furthermore, Tat interacts directly with its target sequence within the LTR and is therefore critical for viral replication. Anti-tat hammerhead ribozymes and non-catalytic control ribozymes targeted to four Tat mRNA target sequences were cloned into plasmid DNAs. Each ribozyme Ribozyme

A ribonucleic acid (RNA) molecule that, like a protein, can catalyze specific biochemical reactions. Examples include self-splicing rRNA and RNase P, both involved in catalyzing RNA processing reactions (that is, the biochemical reactions that convert
, along with the Tat substrate, was transcrit ed by in vitro assay. Ribozyme catalytic activity was measured in an in vitro cleavage reaction followed by analysis of the cleavage products by acrylamide acrylamide /acryl·a·mide/ (ah-kril´ah-mid) a vinyl monomer used in the production of polymers with many industrial and research uses; the monomeric form is a neurotoxin.  gel electrophoresis.
Pamela L. Wall, William H. Jackson
Department of Biology and Geology
University of South Carolina
COPYRIGHT 2003 South Carolina Academy of Science
No portion of this article can be reproduced without the express written permission from the copyright holder.
Copyright 2003 Gale, Cengage Learning. All rights reserved.

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Author:Wall, Pamela L.; Jackson, William H.
Publication:Bulletin of the South Carolina Academy of Science
Article Type:Brief Article
Geographic Code:1U5SC
Date:Jan 1, 2003
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