Amersham Biosciences Simplifies DNA Preparation Process with Innovative Whole Genome Amplification Method.Business Editors/Health/Medical Writers BIOWIRE2K PISCATAWAY, N.J.--(BUSINESS WIRE)--April 10, 2003 GenomiPhi DNA Amplification Kit Enables More Genetic Testing from Limited Source Material Amersham Biosciences, the life sciences business of Amersham plc (LSE LSE - Language Sensitive Editor :, NYSE NYSE See: New York Stock Exchange :, OSE OSE - Open Systems Environment : AHM), today announced the launch of GenomiPhi(TM) DNA Amplification Kit to simplify the DNA DNA: see nucleic acid. DNA or deoxyribonucleic acid One of two types of nucleic acid (the other is RNA); a complex organic compound found in all living cells and many viruses. It is the chemical substance of genes. preparation process and enable high-quality DNA to be prepared from limited biological material. This innovative method has been validated with positive feedback from researchers worldwide for use in cancer research, population genetics, environmental studies, plant genomics, and molecular cloning. The simple isothermal i·so·ther·mal adj. Of, relating to, or indicating equal or constant temperatures. isothermal, isothermic having the same temperature. method employed by GenomiPhi DNA Amplification Kit allows microgram quantities of DNA to be produced overnight from nanogram nanogram /nano·gram/ (ng) (nan?o-gram) one billionth (10-9) of a gram. nan·o·gram n. Abbr. ng One billionth (10-9) of a gram. amounts of starting material. With only one protocol needed for different types of starting material, total hands-on time required by users is just 20 minutes. The isothermal amplification technique utilized by GenomiPhi DNA Amplification Kit also eliminates the need for a thermocycler, which simplifies the customer's workflow. This method offers an advantage over traditional matrix-based purification columns for genomic DNA preparation where the DNA yield depends on sufficient starting quantities of high quality source material. The source material for GenomiPhi DNA Amplification Kit can be purified DNA or lysed cells from whole blood, buccal swabs, cell-blotted paper, plant tissue and microorganisms. The amplified DNA preserves original single nucleotide polymorphism Noun 1. single nucleotide polymorphism - (genetics) genetic variation in a DNA sequence that occurs when a single nucleotide in a genome is altered; SNPs are usually considered to be point mutations that have been evolutionarily successful enough to recur in a (SNP) information and can be used in various applications including SNP genotyping and short tandem repeat A short tandem repeat (STR) in DNA is a class of polymorphisms that occurs when a pattern of two or more nucleotides are repeated and the repeated sequences are directly adjacent to each other. (STR) genotyping, polymerase chain reaction polymerase chain reaction (pŏl`ĭmərās') (PCR), laboratory process in which a particular DNA segment from a mixture of DNA chains is rapidly replicated, producing a large, readily analyzed sample of a piece of DNA; the process is (PCR PCR polymerase chain reaction. PCR abbr. polymerase chain reaction Polymerase chain reaction (PCR) ) and library construction. The GenomiPhi method employs the biochemical properties of Phi29 DNA polymerase, a highly processive enzyme with excellent strand displacement activity and high fidelity, in combination with random-sequence hexamer primers to amplify DNA in an isothermal process. This is the only Phi 29 DNA polymerase-based product that can be sold for nucleic acid research use under applicable patents. "When source material is in limited supply, it is vital that sufficient amounts of high-quality genomic DNA content is achieved simply and cost effectively," said Andy Bertera, Vice President Genomics Product Management at Amersham Biosciences. "Compared to traditional genomic DNA isolation and purification methods, where DNA yield depends on the quantity and quality of the starting material, plus different protocols, GenomiPhi DNA Amplification Kit is a straightforward process for preparing DNA." Amersham Biosciences, the life sciences business of Amersham plc (LSE: NYSE: OSE: AHM), is a world leader in developing and providing integrated systems and solutions for disease research, drug development and manufacture. Our systems are used to uncover the function of genes and proteins, for the discovery and development of drugs and for the manufacture of biopharmaceuticals. The customers for Amersham Biosciences' products and technology are pharmaceutical and biotechnology companies and research and academic institutions, principally in North America, Europe, Latin America and Asia. More information about Amersham Biosciences is available at www.amershambiosciences.com Amersham Biosciences--enabling molecular medicine |
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