Amersham Biosciences Introduces Ettan DIGE Platform for 2-D Fluorescence Difference Gel Electrophoresis.Business Editors, Health & Medical Writers BIOWIRE2K PISCATAWAY, N.J.--(BW HealthWire)--April 23, 2002 Amersham Biosciences A novel method for differential protein expression analysis Amersham Biosciences, the life sciences business of Amersham plc Amersham plc was a UK pharmaceutical company, specializing in medical diagnostics and life science products. The company was formed in 1997 by merging Amersham International (U.K.), Pharmacia Biotech (Sweden) and Nycomed (Norway). (LSE LSE - Language Sensitive Editor :, NYSE NYSE See: New York Stock Exchange :, OSE OSE - Open Systems Environment : AHM AHM Automated Hacking Machines AHM All Hands Meeting AHM Academy for Healthcare Management AHM Atom Heart Mother (Pink Floyd album) AHM Airport Handling Manual AHM Acutely Hazardous Material AHM Anti-Helicopter Mine ) has introduced Ettan(TM) DIGE DIGE Differential In-Gel Electrophoresis DIGE Direct-Injection Gasoline Engine , a new platform for its unique, patented technology for 2-Dimensional Fluorescence Difference Gel Electrophoresis Difference gel electrophoresis (DIGE) is a form of gel electrophoresis where up to three different protein samples can be labeled with fluorescent dyes (for example Cy3, Cy5, Cy2) prior to two-dimensional electrophoresis. (2-D DIGE). 2-D DIGE technology is already used by some 50 leading pharmaceutical and academic laboratories world-wide through an early-access program. With Ettan DIGE, researchers can be confident that protein expression differences are real, biological differences, leading to more reliable and rapid development of disease and drug models. Ettan DIGE is a unique system that brings an as yet unrivalled statistical confidence and level of reliability to 2-D electrophoresis results, as each protein spot has its own internal standard. Ettan DIGE offers proteomics researchers a critical advantage as it enables multiplexing, the separation of multiple samples on a single gel. Multiplexing increases the throughput, reproducibility, and accuracy of protein expression studies. Researchers can now compare, on the same gel, protein samples from normal and diseased tissues, together with drug-treated tissues. This allows detection of the differential expression of proteins in particular disease states, and monitoring of the effects of existing or new drug candidates. The Ettan DIGE system consists of three key components: Amersham Biosciences' proprietary cyanine dye Noun 1. cyanine dye - any of a class of dyes containing a -CH= group linking two heterocyclic rings containing nitrogen; used as sensitizers in photography dye, dyestuff - a usually soluble substance for staining or coloring e.g. fabrics or hair chemistry, CyDye(TM) DIGE fluors (exclusively licensed to Amersham Biosciences from Carnegie Mellon University Carnegie Mellon University, at Pittsburgh, Pa.; est. 1967 through the merger of the Carnegie Institute of Technology (founded 1900, opened 1905) and the Mellon Institute of Industrial Research (founded 1913). ), Typhoon typhoon: see hurricane. (TM) variable-mode gel imager, and DeCyder(TM) differential analysis software. In Ettan DIGE, up to three fluorescently labelled protein samples can be loaded onto the same electrophoresis gel. After 2-D electrophoresis, the gel is scanned using the highly sensitive Typhoon imager. DeCyder software can be used to automatically locate and analyze the protein spots, yielding a very sensitive and accurate measure of differential protein expression. DeCyder derives statistical data intra-gel rather than inter-gel, enabling routine detection of down to less than ten percent differences in expression with over ninety-five percent confidence, within minutes. "We are delighted to provide such a powerful proteomics tool to the research community. We are helping researchers to achieve results they can trust," says Pieter Noordeloos, Vice President Marketing, Proteomics, Amersham Biosciences. "Ettan DIGE brings together our core strengths and proprietary technologies including CyDye fluors, 2-D electrophoresis, imaging, and DeCyder analysis software, all key components of the Ettan DIGE platform." Amersham Biosciences, the life sciences business of Amersham plc (LSE: NYSE: OSE: AHM), is a world leader in developing and providing integrated systems and solutions for disease research, drug development, and manufacture. Our systems are used to uncover the function of genes and proteins, for the discovery and development of drugs, and for the manufacture of biopharmaceuticals. The customers for Amersham Biosciences' products and technology are pharmaceutical and biotechnology companies, and research and academic institutions, principally in North America, Europe, Latin America, and Asia. More information about Amersham Biosciences is available at www.amershambiosciences.com Amersham Biosciences--enabling molecular medicine |
|
Printer friendly
Cite/link
Email
Feedback
Reader Opinion