Airborne particles of the California Central valley alter the lungs of healthy adult rats. (Research).Epidemiologic studies have shown that airborne particulate matter particulate matter
n. Abbr. PM
Material suspended in the air in the form of minute solid particles or liquid droplets, especially when considered as an atmospheric pollutant.
Noun 1. (PM) with a mass median aerodynamic diameter Drug particles for pulmonary delivery are typically characterized by aerodynamic diameter rather than geometric diameter. The velocity at which the drug settles is proportional to the aerodynamic diameter, da. < 10 pm (P[M.sub.10]) is associated with an increase in respiratory-related disease. However, there is a growing consensus that particles < 2.5 pm (P[M.sub.2.5]), including many in the ultrafine (< 0.1 pm) size range, may elicit greater adverse effects. PM is a complex mixture of organic and inorganic compounds Tentative listing related to this page, inorganic compounds by element (presently under construction), as well as .
This list is not necessarily complete or up to date – if you see an article that should be here but isn't (or one that shouldn't be here but is), please update ; however, those components or properties responsible for biologic effects on the respiratory system respiratory system: see respiration.
Organ system involved in respiration. In humans, the diaphragm and, to a lesser extent, the muscles between the ribs generate a pumping action, moving air in and out of the lungs through a have yet to be determined. During the fall and winter of 2000-2001, healthy adult Sprague-Dawley rats were exposed in six separate experiments to filtered air or combined fine (P[M.sub.2.5]) and ultrafine portions of ambient PM in Fresno, California, enhanced approximately 20-fold above outdoor levels. The intent of these studies was to determine if concentrated fine/ultrafine fractions of PM are cytotoxic cy·to·tox·ic
Of, relating to, or producing a toxic effect on cells.
cyto·tox·ic and/or proinflammatory in the lungs of healthy adult rats. Exposures were for 4 hr/day for 3 consecutive days. The mean mass concentration of particles ranged from 190 to 847 [micro]g/[m.sup.3]. PM was enriched primarily with ammonium nitrate, organic and elemental carbon, and metals. Viability of cells recovered by bronchoalveolar lavage Bronchoalveolar lavage
A way of obtaining a sample of fluid from the airways by inserting a flexible tube through the windpipe. Used to diagnose the type of lung disease. (BAL (1) (Basic Assembly Language) The assembly language for the IBM 370/3000/4000 mainframe series.
(2) (Branch And Link) An instruction used to transfer control to another part of the program.
BAL - Basic Assembly Language ) from rats exposed to concentrated PM was significantly decreased during 4 of 6 weeks, compared with rats exposed to filtered air (p < 0.05). Total numbers of BAL cells were increased during 1 week, and neutrophil neutrophil /neu·tro·phil/ (noo´tro-fil)
1. a granular leukocyte having a nucleus with three to five lobes connected by threads of chromatin, and cytoplasm containing very fine granules; cf. heterophil.
2. numbers were increased during 2 weeks. These observations strongly suggest exposure to enhanced concentrations of ambient fine/ultrafine particles in Fresno is associated with mild, but significant, cellular effects in the lungs of healthy adult rats. Key words: concentrated ambient particles, fine particles, particulate matter, pulmonary inflammation, ultrafine particles.
Epidemiologic studies have shown a relationship between increased levels of particulate matter (PM) with a mass median aerodynamic diameter < 10 [micro]m (P[M.sub.10]) and an increase in respiratory-related morbidity and mortality Morbidity and Mortality can refer to:
tr.v. im·pli·cat·ed, im·pli·cat·ing, im·pli·cates
1. To involve or connect intimately or incriminatingly: evidence that implicates others in the plot.
2. in the toxicity of inhaled PM in animal studies.
Although a wealth of epidemiologic information suggests that PM is involved in adverse health effects, the physical or chemical properties responsible remain unknown. Part of the difficulty in identifying such properties lies in the fact that creating "real-world" particles to study their effects in animals or humans in a controlled setting is difficult to do. Several studies have used particles from individual sources that contribute to the ambient PM. However, the disadvantage of exposure to particles collected from specific emission sources, including residual oil fly ash, coal fly ash, or diesel exhaust PM, is that these sources make a small contribution to the overall PM mix and are not likely to be representative of ambient particle exposures. In addition, such studies cannot effectively reproduce the ultrafine fraction of particles originally present at the time of particle formation, thus making it impossible to study their effects on biologic systems.
Recent advances in engineering have made it possible to concentrate particles in real time from the ambient environment to study health effects. These systems have facilitated the concentration of particles ranging in size from 0.1 to 2.5 pm; however, concentrating ultrafine particles (< 0.1 [micro]m) has been more problematic (Sioutas et al. 1997). Original particle concentrators included the Harvard Fine Particle Concentrator (Sioutas et al. 1995) and a concentrator that uses centrifugal force (Gordon et al. 1999). However, these concentrators do not enhance the concentration of particles below approximately 0.1 [micro]m (i.e., the ultrafine particles). The concentrator used for this study is designed to concentrate both fine and ultrafine PM (Kim et al. 2000). The system uses hydration hydration /hy·dra·tion/ (hi-dra´shun) the absorption of or combination with water.
1. The addition of water to a chemical molecule without hydrolysis.
2. to enlarge particles to supermicrometer droplets with supersaturation supersaturation,
n the addition to or presence of an ingredient in a solution in greater quantity than the solvent can permanently take up. and condensation. The particles are then separated by size using a virtual impactor and returned to their original (ambient) size by passing through a diffusion dryer to remove particle-bound water.
Normal rats (Clarke et al. 1999; Gordon et al. 1998; Kodavanti et al. 2000), as well as rats with chronic bronchitis chronic bronchitis
Inflammation of the bronchial mucous membrane, characterized by cough, hypersecretion of mucus, and expectoration of sputum over a long period of time and associated with increased vulnerability to bronchial infection. (Clarke et al. 1999; Kodavanti et al. 2000) or pulmonary hypertension Pulmonary Hypertension Definition
Pulmonary hypertension is a rare lung disorder characterized by increased pressure in the pulmonary artery. The pulmonary artery carries oxygen-poor blood from the lower chamber on the right side of the heart (right (Gordon et al. 1998), have been used to evaluate the effects of concentrated ambient particles (CAPs) on pulmonary function and inflammation. However, these studies have produced contrasting results. A potential deficiency in these studies was the inability to concentrate ultrafine (< 0.1 [micro]m) particles from the environment.
The Central Valley of California has some of the highest ambient particle concentrations found in the United States, especially in the P[M.sub.2.5] range. Contributors to ambient PM in the Central Valley include agricultural and ranching activities, fires, wind-blown dust, diesel and gasoline engine exhaust, power plant emissions, and home heating (Watson et al. 2000). Pinkerton et al. (2000) recently examined the lungs of deceased young Hispanic males from the California Central Valley (Fresno, CA) who had been healthy and died of nonrespiratory causes. They found principal sites of particle deposition and/or retention in the lungs to be terminal bronchioles and first-generation respiratory bronchioles associated with thickened thick·en
tr. & intr.v. thick·ened, thick·en·ing, thick·ens
1. To make or become thick or thicker: Thicken the sauce with cornstarch. The crowd thickened near the doorway.
2. walls and alveolar alveolar /al·ve·o·lar/ (al-ve´o-lar) [L. alveolaris ] pertaining to an alveolus.
Relating to an alveolus. septa septa /sep·ta/ (sep´tah) [L.] plural of septum.
Septum (plural, septa)
The dividing partition in the nose that separates the two nostrils. It is composed of bone and cartilage. along with dust-laden macrophages Macrophages
White blood cells whose job is to destroy invading microorganisms. Listeria monocytogenes avoids being killed and can multiply within the macrophage. (Pinkerton et al. 2000). Such observations strongly suggest that particles may be retained in the lungs and result in structural alterations over time.
The composition and concentration of fine and ultrafine fractions of P[M.sub.10] vary in the Central Valley with seasonal and atmospheric conditions. Therefore, studies described here were completed during two different times of the year to examine varied compositions of PM and their potential effects on the respiratory system. Concentration of the ambient particles in Fresno by 20-fold allowed for controlled exposure of healthy, adult rats to elevated levels of particles. The intent of this study was to determine if concentrated fine/ultrafine fractions of ambient P[M.sub.2.5] are cytotoxic and/or proinflammatory in the lungs of healthy adult rats. After 3 days of exposure (4 hr/day), we measured bronchoalveolar lavage (BAL) cell viability and total cells, as well as the number and relative proportion of macrophages, neutrophils neutrophils (ner·ō·trōˑ·filz),
n.pl white blood cells with cytoplasmic granules that consume harmful bacteria, fungi, and other foreign materials. , and lymphocytes Lymphocytes
Small white blood cells that bear the major responsibility for carrying out the activities of the immune system; they number about 1 trillion. from rats exposed to CAPs.
Materials and Methods
Animals. We purchased 9-10-week-old male Sprague-Dawley rats, free of respiratory disease, from Harlan (San Diego, CA) and quarantined them for 1 week before exposure to CAPs. The study designation, dates of exposure, age of animals, and number of rats per group are shown in Table 1. Animals were handled in accordance with standards established by the U.S. Animal Welfare Acts as set forth in the National Institutes of Health guidelines (Institute of Laboratory Animal Resources 1996) as well as the Animal Care and Use committees of both the University of California, Davis The University of California, Davis, commonly known as UC Davis, is one of the ten campuses of the University of California, and was established as the University Farm in 1905. and California State University, Fresno The campus sits at the foot of the Sierra Nevada mountain range in the San Joaquin Valley. Fresno County is the sixth largest metropolitan area in California. The university is within an hour's drive of many mountain and lake resorts and within a three- or four-hour drive of both Los . The rats were housed in plastic cages with TEK-Chip pelleted paper bedding (Harlan Teklad, Madison, WI) and maintained on a 12-hr light/12-hr dark cycle. All animals had access to Laboratory Rodent Diet 5001 (LabDiet, Brentwood, MO) ad libitum ad libitum
ad libitum feeding
food available at all times with the quantity and frequency of consumption being the free choice of the animal. before, during, and after exposures. All animals had access to water ad libitum, except during each daily exposure period because of the design of the exposure chambers.
Particle concentrator system. Animals were exposed either to room air passed through an activated carbon-impregnated filter media pad (Dayton Electric Manufacturing Co., Niles, IL) or to fine and ultrafine CAPs generated by portable concentrators developed over the past 2 years at the University of Southern California The U.S. News & World Report ranked USC 27th among all universities in the United States in its 2008 ranking of "America's Best Colleges", also designating it as one of the "most selective universities" for admitting 8,634 of the almost 34,000 who applied for freshman admission . These portable Versatile Aerosol Concentration Enrichment Systems (VACES) are based on previously described technology (Kim et al. 2000; Sioutas et al. 1999). These systems are able to enrich the concentration of particles in the complete size range of 0.01-10 [micro]m by up to a factor of 40, depending on the output flow rate. By incorporating size-selective inlets, the VACES can provide CAPs in selected size ranges. These systems are compact in size and modular in design, thus allowing their use in mobile exposure platforms. The performance of these systems has been described in detail by Kim et al. (2001a, 2001b).
Concentration enrichment of particles with aerodynamic diameters < 2.5 [micro]m is accomplished by first drawing air samples through two parallel lines, each sampling at 110 L/min for a total flow of 220 L/min (Figure 1). Each line has a 2.5-[micro]m cut-point preimpactor, to remove essentially all of the particles > 2.5 [micro]m from the flow. PM is drawn through a saturation-condensation system that grows particles to 2-3 pm droplets, which are then concentrated by virtual impaction from a total flow of 220 L/min to a flow of 10 L/min, to concentrate particles by a factor of 22. Two diffusion dryers are used downstream of the virtual impactors to remove excess water vapor and return concentrated particles to their original size, before delivering them to the exposure chambers.
[FIGURE 1 OMITTED]
Detailed laboratory characterization of individual components of the VACES has demonstrated the ability of this system to preserve particle mass, number, and chemical species during the concentration enrichment process (Kim et al. 2001b). Experimental characterization of the VACES demonstrated that concentration enrichment is accomplished with high efficiency and minimal particle loss over the range of particle sizes and chemical compositions encountered in ambient atmospheres. Field evaluation, described by Kim et al. (2001a), also demonstrated that volatile species, such as ammonium nitrate, are preserved throughout the supersaturation and concentration-enrichment processes. Ultrafine particles are concentrated without substantial changes in their compactness or density, as measured by fractal dimension (mathematics) fractal dimension - A common type of fractal dimension is the Hausdorff-Besicovich Dimension, but there are several different ways of computing fractal dimension. analysis (Kim et al. 2001a).
The VACES was installed in the animal vivarium located on the campus of California State University, Fresno. The three-story building that houses the vivarium is located in the northeastern section of campus. This location is close to the secondary Supersite station monitored by the U.S. Environmental Protection Agency Environmental Protection Agency (EPA), independent agency of the U.S. government, with headquarters in Washington, D.C. It was established in 1970 to reduce and control air and water pollution, noise pollution, and radiation and to ensure the safe handling and in Fresno. Ambient air for each exposure was drawn from the roof of the building, and the aerosol was delivered to the VACES via an 8-m-long, 7.62-cm-diameter duct. At a flow rate of 220 L/min, the aerosol residence time in the duct before reaching the VACES was about 10 sec, rapid enough to avoid particle loss on the duct walls from deposition by settling (for larger particles) or diffusion (for smaller particles). The concentrated aerosol was supplied to whole-body animal exposure chambers.
Fabrication fabrication (fab´rikā´shn),
n the construction or making of a restoration. and characterization of exposure chambers. Exposure chambers were constructed using standard animal housing cages made of polycarbonate A category of plastic materials used to make a myriad of products, including CDs and CD-ROMs. measuring 20 x 43 x 18 cm. The metal top of the cage that held food and water was replaced with a lid designed to deliver aerosols into the chamber (Figure 2). Air passing through the cyclone-designed orifice orifice /or·i·fice/ (or´i-fis)
1. the entrance or outlet of any body cavity.
2. any opening or meatus.orific´ial
aortic orifice created a circular flow into the chamber. A perforated metal sheet consisting of 1.7-mm-diameter holes and 23% open area placed in the lid allowed airflow to be distributed along the top of the cage in a uniform manner. Air was drawn out of the chamber by two tubes located at the bottom of the cage. Holes were placed evenly along the exit tubes to draw air out along the length of the chamber. To test airflow through the chambers, before their use in Fresno, smoke was admitted into the chamber and observed as it moved from the top to the bottom exit tubes. Repeated testing demonstrated filling and emptying of the chamber with smoke was evenly distributed from the top to bottom of each chamber. Airflow ranging from 2 L/min to 15 L/min demonstrated uniform mixing of smoke particles throughout the chamber.
[FIGURE 2 OMITTED]
During the studies in Fresno, the system was operated at a negative pressure (up to 7 inches of water) to deliver fine and ultrafine aerosols with minimal loss. Each chamber has an independent flow regulator with the total flow from the four chambers equal to the total output of the VACES.
Physicochemical physicochemical /phys·i·co·chem·i·cal/ (fiz?i-ko-kem´ik-il) pertaining to both physics and chemistry.
1. Relating to both physical and chemical properties. characteristics of concentrated PM. Animal exposures to concentrated fine and ultrafine PM in Fresno are summarized in Table 1. Experiments were conducted during 3 consecutive weeks in the fall of 2000 and 3 consecutive weeks in the winter of 2001. Each week animals were exposed for 4 hr/day for 3 days. Of the 10 L/min of concentrated aerosol generated, 2.3 L/min was diverted into a sampling manifold to determine physical and chemical characteristics of the exposure aerosol, and the remaining 7.7 L/min was supplied to the four animal exposure chambers.
Particle mass and elemental composition were measured by collecting concentrated PM on 37-mm Teflon filters (PTEF, 2-[micro]m pore; Gelman Science, Ann Arbor, MI) at a flow rate of 1 L/min. The retention efficiency of these filters, even for the most penetrating particles (0.2-0.3 [micro]m), is > 99%. The Teflon filters were weighed before and after each experiment using a Mettler 5 microbalance mi·cro·bal·ance
A balance designed to weigh very small loads, up to 0.1 gram.
Noun 1. microbalance - balance for weighing very small objects
balance - a scale for weighing; depends on pull of gravity (model MT 5; Mettler-Toledo Inc., Highstown, NJ), under controlled relative humidity relative humidity
The ratio of the amount of water vapor in the air at a specific temperature to the maximum amount that the air could hold at that temperature, expressed as a percentage. (40-45%) and temperature (22-24[degrees]C) in the Aerosol Laboratory at the University of Southern California. At the end of each 3-day experiment, filters were stored at constant humidity and temperature for 24 hr before weighing to ensure removal of particle-bound water. After Teflon filters were weighed, they were analyzed by X-ray fluorescence to determine the concentrations of particle-bound trace elements Trace elements
A group of elements that are present in the human body in very small amounts but are nonetheless important to good health. They include chromium, copper, cobalt, iodine, iron, selenium, and zinc. Trace elements are also called micronutrients. and metals.
Concentrations of inorganic ions (i.e., sulfate sulfate, chemical compound containing the sulfate (SO4) radical. Sulfates are salts or esters of sulfuric acid, H2SO4, formed by replacing one or both of the hydrogens with a metal (e.g., sodium) or a radical (e.g., ammonium or ethyl). and nitrate), elemental carbon (EC), and organic carbon (OC) were determined by collecting particles on 37-mm prebaked quartz filters (Pallflex Corp., Putnam, CT). Particles were collected on quartz filters at a flow rate of 1 L/min, parallel to the Teflon filter used for gravimetric and metal analysis. At the end of each 3-day experiment, a 1-[cm.sup.2] circular section of the quartz filter was removed from the center of the filter and analyzed by thermo-analysis to determine EC and OC content using a process described in detail by Fung (1990). The remainder of the filter was extracted using a mixture of 0.1 mL of ethanol and 5 mL of distilled deionized water and analyzed by ion chromatography to determine the concentrations of sulfate and nitrate. Particle number concentrations were measured continuously throughout each exposure period with a TSI TSI Total Solar Irradiance (sum solar light in energy per unit of time)
TSI Trading Standards Institute (UK)
TSI Transportation Safety Institute (US DOT) 3022 condensation particle counter, sampling at a flow rate of 0.3 L/min.
BAL fluid analysis. Immediately after exposure on the third day, rats were anesthetized a·nes·the·tize also a·naes·the·tize
tr.v. a·nes·the·tized, a·nes·the·tiz·ing, a·nes·the·tiz·es
To induce anesthesia in.
a·nes by intraperitoneal injection of pentobarbital pentobarbital /pen·to·bar·bi·tal/ (pen?to-bahr´bi-tal) a short- to intermediate-acting barbiturate; the sodium salt is used as a hypnotic and sedative, usually presurgery, and as an anticonvulsant. (100-150 mg/kg body weight) and exsanguinated via the abdominal aorta abdominal aorta Anatomy The portion of the aorta that begins below the diaphragm, extends to the bifurcation of the iliac arteries, and supplies blood to the abdominal viscera, pelvic organs and legs Branches Inferior phrenic, lumbar, celiac trunk, superior . The trachea trachea (trā`kēə) or windpipe, principal tube that carries air to and from the lungs. It is about 4 1-2 in. (11.4 cm) long and about 3-4 in. (1.9 cm) in diameter in the adult. was cannulated can·nu·late also can·u·late
tr.v. can·nu·lat·ed, can·nu·lat·ing, can·nu·lates
To insert a cannula into (a bodily cavity, duct, or vessel), as for the drainage of fluid or the administration of medication.
adj. and the lungs lavaged with [Ca.sup.2+]/[Mg.sup.2+]-free phosphate-buffered saline (PBS PBS
in full Public Broadcasting Service
Private, nonprofit U.S. corporation of public television stations. PBS provides its member stations, which are supported by public funds and private contributions rather than by commercials, with educational, cultural, ; pH 7.4) at a volume equal to 35 mL/kg body weight. Three in-and-out lavages were performed using the same fluid. BAL fluid was centrifuged at 2,000 rpm for 10 min at 4[degrees]C. Supernatant supernatant /su·per·na·tant/ (-na´tant) the liquid lying above a layer of precipitated insoluble material.
the liquid lying above a layer of precipitated insoluble material. was removed, and the cell pellet was resuspended in [Ca.sup.2+]/[Mg.sup.2+]-free PBS. Resuspended cells (0.1 mL) were used to determine total cell count and viability. Cell viability was measured by exclusion of 0.4% trypan blue try·pan blue
An acid dye used for staining of the reticuloendothelial system, the kidney tubules, and cells in tissue culture.
a supravital stain and a stain for amyloid. (Sigma, St. Louis, MO), an indicator of irreversible loss of plasma membrane plasma membrane
See cell membrane. integrity. A second aliquot aliquot (al-ee-kwoh) adj. a definite fractional share, usually applied when dividing and distributing a dead person's estate or trust assets. (See: share) of cells was centrifuged using a Shandon Cytospin (Thermo Shandon, Inc., Pittsburgh, PA) to prepare cell differential slides. The slides were dried at room temperature and stained with HEMA HEMA Hydroxyethyl Methacrylate
HEMA Hollandsche Eenheidsprijzen Maatschappij Amsterdam (Dutch international retail organization)
HEMA Hydroxy Ethyl Methacrylate
HEMA Health and Environment Ministers of the Americas
HEMA Hemophilia, Classic 3 (Biochemical Sciences, Inc., Swedesboro, NJ). Macrophages, neutrophils, and lymphocytes were counted using light microscopy (1,000 cells/sample).
Statistics. Each experiment consisted of a filtered air-exposed group and a CAPs-exposed group for all 6 weeks. The data were analyzed by analysis of variance. Comparisons among exposure groups were evaluated by Fisher's protected least significant difference test. Comparisons were considered significant if a value of p < 0.05 was observed. Statistical analysis was performed with StatView 5.0.1 (SAS Institute Inc., Cary, NC).
Characterization of exposure chambers. To test the exposure chambers, the flow rate through each exposure chamber was set at 2.5 L/min, and fluorescein fluorescein /flu·o·res·ce·in/ (fldbobr-res´en) a fluorescing dye; its sodium salt is used as a tracer in retinal angiography and as a diagnostic aid for revealing corneal trauma and fitting contact lenses. particles were generated with a nebulizer nebulizer /neb·u·liz·er/ (neb´u-li?zer) atomizer; a device for throwing a spray.
n. . Particles were heated and passed through a diffusion dryer to remove water vapor. Particles were collected by placing round microscope cover slips on six cooled brass rods. The microscope slides were placed in pH 10 water and counted in a fluorometer fluorometer /flu·o·rom·e·ter/ (fldbobr-rom´e-ter) the instrument used in fluorometry, consisting of an energy source (e.g., a mercury arc lamp or xenon lamp) to induce fluorescence, filters or monochromators for selection of the . The large particles [3.1 [micro]m mass median aerodynamic diameter (MMAD MMAD Mass Median Aerodynamic Diameter
MMAD Musicians Making A Difference
MMAD Maxi-Minis And Databases
MMAD Medical Marijuana Access Division
MMAD Minerva McGonagall Albus Dumbledore (Harry Potter fanfiction) )] were collected on the cover slips and showed 13% variability. Smaller particles, with a MMAD of 0.56 [micro]m, were generated and showed 10% variability. Cages were swabbed, and the swabs were analyzed in a fluorometer but exhibited no activity over background. This demonstrated that particles were not depositing on chamber walls.
Characteristics of concentrated aerosols. Physicochemical characteristics of concentrated particles during fall 2000 and winter 2001 are shown in Figures 3-6 and in Table 2. These data represent averages over each 3-day period for each week of exposure.
Exposures during the fall of 2000 demonstrated remarkably consistent particle numbers, varying between 1.1 x [10.sup.5] and 1.2 x [10.sup.5] particles/[cm.sup.3]. However, the average mass concentration of the concentrated aerosol varied substantially, ranging from 260 to 847 [micro]g/[m.sup.3], as shown in Table 2. Ammonium nitrate and OC were the most prevalent constituents of concentrated fine/ultrafine PM in Fresno, accounting for approximately 60-80% of the total PM mass. The highest particle mass concentration during the fall was observed in the first week, with this week also demonstrating the highest fall concentrations for individual PM species, especially nitrates and EC. Ammonium nitrate concentration was 24% of the total PM mass during the second week and 57% of the total PM mass during the third week of fall. EC concentration during the first week of fall was 10 times higher than during the next 2 weeks of exposure. OC concentration was relatively consistent over the 3-week fall period, ranging from 80 to 141 [micro]g/[m.sup.3]. The concentration of trace metals was similar during the first 2 weeks (38-42 [micro]g/[m.sup.3]) but decreased to 14 [micro]g/[m.sup.3] during the third week. Figure 4 shows mass concentrations of the most predominant trace elements and metals, with Al, Si, S, Ca, and Fe having the highest concentrations. With the exception of sulfur, metal and trace element concentrations during the first 2 weeks of exposure were similar. An increase in sulfur concentration during the first week of fall may have been due to the greater ammonium sulfate concentration detected during the same week (Figure 3A), because sulfur measured by X-ray fluorescence is also part of the ammonium sulfate measured by ion chromatography. The overall higher nitrate and sulfate concentrations for the first week of fall were most likely due to increased photochemical photochemical
in laser treatment, the laser light is absorbed and converted into chemical energy. activity coupled with prevailing quasistagnant meteorologic me·te·or·ol·o·gy
The science that deals with the phenomena of the atmosphere, especially weather and weather conditions.
[French météorologie, from Greek conditions during this period.
[FIGURE 4 OMITTED]
The total mass and particle number concentrations measured during the winter 2001 exposures in Fresno (Table 2) were remarkably similar to those of the fall exposures. Average total mass concentrations were 815, 190, and 371 [micro]g/[m.sup.3], for the first, second, and third weeks, respectively. Particle number concentration did not vary substantially for the 3 weeks of exposure, as indicated by Table 2. These concentrations were similar to those measured in the fall, varying from 0.9 x [10.sup.5] to 1.2 x [10.sup.5] particles/[cm.sup.3]. Nitrate and OC were the predominant PM constituents, accounting for 46-69% of the total CAPs mass each week. Nitrate and sulfate concentrations were substantially higher during the first week, compared with the second and third weeks of winter. The concentrations of combined trace elements and metals were 16, 14, and 8 [micro]g/[m.sup.3] for the first, second and third weeks of exposure, respectively. Sulfur concentration was appreciably higher during the first week, along with a higher ammonium sulfate concentration. CAPs concentrations for metals of primarily crustal crust·al
Of or relating to a crust, especially that of the earth or the moon.
Adj. 1. crustal - of or relating to or characteristic of the crust of the earth or moon origin, such as Al, Si, K, Ca, and Ti were similar during the first 2 weeks of exposure but lower during the third week (Figure 6). Concentrations of primarily combustion-generated metals, such as Fe, Zn, and Pb were similar during the 3-week exposure period (Figure 6).
[FIGURE 6 OMITTED]
Table 3 provides a summary of the CAPs mass and chemical and elemental composition during all 6 weeks of exposures. Each species (including particle number and mass) is represented by its arithmetic mean (mathematics) arithmetic mean - The mean of a list of N numbers calculated by dividing their sum by N. The arithmetic mean is appropriate for sets of numbers that are added together or that form an arithmetic series. and coefficient of variation Coefficient of Variation
A measure of investment risk that defines risk as the standard deviation per unit of expected return. .
CAPs-induced BAL changes. Total BAL cells for each exposure week are shown in Figure 7A. Exposure to CAPs during the first week of winter was associated with a significant increase in the total number of cells recovered by lavage lavage /la·vage/ (lah-vahzh´)
1. the irrigation or washing out of an organ, as of the stomach or bowel.
2. to wash out, or irrigate.
n. (1.2 x [10.sup.5] cells/mL) compared with filtered air controls (0.8 x [10.sup.5] cells/mL) (Figure 7A). The percentage of macrophages in the BAL fluid after exposure to filtered air or CAPs was greater than 99.3% or 98.8%, respectively (Table 4). The percentage of macrophages with CAPs exposure was only significantly reduced in the second week of the fall season (Table 4). Rats exposed to CAPs in the first week of the winter season had a significantly higher number of BAL macrophages compared with rats exposed only to filtered air (Figure 7B). There was not a significant change in macrophage macrophage /mac·ro·phage/ (mak´ro-faj) any of the large, mononuclear, highly phagocytic cells derived from monocytes that occur in the walls of blood vessels (adventitial cells) and in loose connective tissue (histiocytes, phagocytic number during the other 5 exposure weeks. In 5 of 6 exposure weeks, rats exposed to CAPs had an apparent increase in the number of neutrophils compared with rats exposed only to filtered air (Figure 7C). However, statistically significant increases in the number of neutrophils were noted only in rats exposed to CAPs during the first week of fall (2.3-fold increase) and the first week of winter (3.0-fold increase) (Figure 7C). During these same 2 weeks, the highest levels of PM mass, nitrate, and OC were also observed. The percentage of BAL neutrophils was apparently increased in animals exposed to CAPs during five of six exposures in fall and winter, although statistical significance was achieved only in the first week of the winter season (Table 4). We also noted that lymphocytes during 3 of 6 exposure weeks displayed a trend of increased proportions, although statistically significant changes between control and CAPs groups were not attained (Table 4). CAPs exposure was also associated with a trend toward an increase in the total number of BAL lymphocytes in all 6 weeks (Figure 7D), although statistical significance was not achieved.
[FIGURE 7 OMITTED]
BAL cell permeability, an indicator of decreased membrane integrity, was measured for all 6 weeks of particle exposure (Figure 8). Permeable BAL cells from rats exposed to filtered air ranged from 6% to almost 11% (Figure 8). With CAPs exposure, the proportion of nonviable nonviable /non·vi·a·ble/ (-vi´ah-b'l) not capable of living.
Not capable of living or developing independently. Used especially of an embryo or fetus. BAL cells increased significantly over matched control matched study, matched control
a comparison between groups in which each subject animal is matched by a comparable animal in terms of age and all other measurable parameters. Called also matched or paired control. animals in all 3 weeks of the fall and in the third week of winter (Figure 8). As much as a 242% increase in nonviable cells over control values was observed (Figure 8). Although the percentage of nonviable cells in the second week of winter was increased by expo, sure to CAPs, this did not attain a level of statistical significance.
[FIGURE 8 OMITTED]
The intent of this study was to determine if CAPs, including both fine (< 2.5 [micro]m) and ultrafine (< 0.1 [micro]m) particles, would be cytotoxic and/or proinflammatory in the lungs of healthy adult rats. In this study we used a unique particle concentrator, which is able to concentrate particles in both fine and ultrafine sizes to identify specific biologic responses. During 6 weeks of particle exposure in Fresno, California, particle numbers were remarkably consistent, whereas the average mass of CAPs and nitrate, sulfate, and EC concentrations varied substantially.
The most striking findings of this study were consistent cytotoxic and inflammatory responses associated with exposure to CAPs. Although a clear dose-dependent response cannot be established to explain cellular changes such as neutrophil influx in the lungs as measured by BAL, the two highest mass concentrations observed during the course of these exposures were associated with a significant increase in numbers of total cells and/or neutrophils recovered from the lungs by BAL. Lung cytotoxicity cytotoxicity /cy·to·tox·ic·i·ty/ (si?to-tok-sis´i-te) the degree to which an agent possesses a specific destructive action on certain cells or the possession of such action. measured in the BAL fluid of animals exposed to CAPs was also significantly increased during 4 of 6 exposure weeks, but this measure of cytotoxicity was not dependent upon particle mass. These CAPs exposures were for only 4 hr/day for 3 days, whereas humans are exposed to PM for more extensive periods of time, albeit at lower concentrations. All exposures were to the ambient mixture of particles present in Fresno.
Prior experiments with CAPs in other regions of the United States have also shown variable results in the induction of pulmonary inflammation. Rats exposed to concentrations of CAPs as high as 350 [micro]g/[m.sup.3] for 3 hr in Tuxedo, New York Tuxedo is a town located in Orange County, New York. As of the 2000 census, the town had a total population of 3,334. The town is in the southeastern part of the county. NY Route 17 and the New York State Thruway (Interstate 87) pass through the town. , did not show an increase in lung inflammation, either 3 hr or 24 hr after exposure (Gordon et al. 1998). However, a recent study described by Saldiva et al. (2002) demonstrated that short-term exposure to Boston, Massachusetts, CAPs induced an inflammatory response in the lungs of normal rats and in rats with chronic bronchitis. A modest increase in BAL neutrophils was reported in dogs exposed to CAPs in Boston (Clarke et al. 2000). CAPs have also been shown to induce mild pulmonary inflammation in healthy humans at concentrations as high as 311 [micro]g/[m.sup.3] in Chapel Hill, North Carolina Chapel Hill is a town in North Carolina and the home of the University of North Carolina at Chapel Hill (UNC-CH), the oldest state-supported university in the United States. As of the 2000 census, it had a population of 48,715. As of 2004 its estimated population was 52,440. (Ghio et al. 2000). Preliminary studies in Los Angeles demonstrated that elderly humans exposed to CAPs had slight effects on blood oxygenation oxygenation /ox·y·gen·a·tion/ (ok?si-je-na´shun)
1. the act or process of adding oxygen.
2. the result of having oxygen added. , cardiac arrhythmia cardiac arrhythmia
See cardiac dysrhythmia.
An irregular heart rate or rhythm.
Mentioned in: Holter Monitoring, Stress Test
cardiac arrhythmia , and airway inflammation (Gong et al. 2002a). Further studies demonstrated that exposure of healthy and asthmatic adult humans to fine CAPs in Los Angeles resulted in small acute effects (Gong et al. 2002b). The differences in response to CAPs by humans, dogs, and rats may be due to differences in composition of the particles inhaled in the different regions of the United States, differences in species sensitivity, and/or differences in ventilation rates. However, in each of these reported studies, ultrafine particles were not concentrated. Therefore, the entire range of particle sizes found in an ambient mixture was not concentrated in these previous studies.
Fresno is located in the San Joaquin Valley Noun 1. San Joaquin Valley - a vast valley in central California known for its rich farmland
Calif., California, Golden State, CA - a state in the western United States on the Pacific; the 3rd largest state; known for earthquakes and has some of the highest P[M.sub.10] concentrations in the United States. Chow et al. (1992, 1993) has described the seasonal variability and physicochemical characteristics of PM in Fresno. During the winter, P[M.sub.10] has a dominant P[M.sub.2.5] fraction that consists primarily of carbonaceous car·bo·na·ceous
Consisting of, containing, relating to, or yielding carbon.
of, resembling, or containing carbon
Adj. 1. constituents, ammonium nitrate, and ammonium sulfate. During the summer and early fall, P[M.sub.10] is elevated by windblown dust. These particles are dominated by fugitive mineral dusts primarily associated with coarse (2.5-10 [micro]m) particles but also found in the fine fraction. Nighttime transport of particles via a jet stream from the San Francisco Bay area “Bay Area” redirects here. For other uses, see Bay Area (disambiguation).
The San Francisco Bay Area, colloquially known as the Bay Area or The Bay into the San Joaquin Valley during spring, summer, and fall further contribute to the makeup of pollutants found in the region (Smith et al. 1981a, 1981b). Pollutants can also accumulate during the winter because of periods of stagnation Stagnation
A period of little or no growth in the economy. Economic growth of less than 2-3% is considered stagnation. Sometimes used to describe low trading volume or inactive trading in securities.
A good example of stagnation was the U.S. economy in the 1970s. accompanied by strong inversions lasting as long as 20 days (Chow et al. 1992).
The mechanisms by which ultrafine particles may be more toxic than fine particles remain unknown, although a number of studies have provided insight into this problem. Ferin et al. (1992) demonstrated that when rats were exposed to ultrafine titanium dioxide (Ti[O.sub.2]) particles, pulmonary inflammation was induced. However, rats exposed to a mass of fine Ti[O.sub.2] equal to that used in the ultrafine Ti[O.sub.2] exposures did not develop lung inflammation (Ferin et al. 1992). In a recent study (Donaldson et al. 2002) rats were exposed to ultrafine or fine carbon black particles, and only those rats exposed to the ultrafine particles exhibited an increase in BAL neutrophils. This increase in pulmonary inflammation in rats exposed to ultrafine carbon black was not due to trace amounts of redox-active transition metals present in the particles (Donaldson et al. 2002). Additional studies described by Donaldson et al. (2002) demonstrated a linear correlation between particle surface area and extent of lung inflammation. However, studies described by Smith et al. (2000) showed that induction of the proinflammatory cytokine Cytokine
Any of a group of soluble proteins that are released by a cell to send messages which are delivered to the same cell (autocrine), an adjacent cell (paracrine), or a distant cell (endocrine). interleukin-8 in human lung epithelial cells Epithelial cells
Cells that form a thin surface coating on the outside of a body structure.
Mentioned in: Corneal Transplantation was dependent upon the amount of bioavailable iron in coal fly ash and not on surface area of the ash particles. Current theories on how particles, including those in the ultrafine size range, induce an inflammatory response in the lungs include either an oxidative stress oxidative stress,
n an imbalance of the prooxidant antioxidant ratio in which too few antioxidants are produced or ingested or too many oxidizing agents are produced. or particle interactions with cell-surface receptors. Either of these possibilities may activate signaling in macrophages or epithelial cells, resulting in proinflammatory cytokine and chemokine chemokine /che·mo·kine/ (ke´mo-kin) any of a group of low molecular weight cytokines identified on the basis of their ability to induce chemotaxis or chemokinesis in leukocytes (or in particular populations of leukocytes) in inflammation. production. Other possible explanations for the greater toxicity of ultrafine particles relative to fine particles include decreased ability of macrophages to phagocytose phag·o·cy·tose
to envelop and destroy bacteria and other foreign material; phagocytize. ultrafine particles (Kreyling and Scheuch 2000) or inhibition of phagocytosis phagocytosis: see endocytosis.
A mechanism by which single cells of the animal kingdom, such as smaller protozoa, engulf and carry particles into the cytoplasm. by ultrafine particles (Donaldson et al. 2001; Lundborg et al. 2001). Renwick et al. (2001) demonstrated that ultrafine Ti[O.sub.2] particles impaired alveolar macrophage alveolar macrophage
A vigorously phagocytic macrophage on the epithelial surface of lung alveoli that ingests carbon and other inhaled particulate matter. Also called coniophage, dust cell. phagocytosis to a greater extent than did fine Ti[O.sub.2] particles.
In vitro in vitro /in vi·tro/ (in ve´tro) [L.] within a glass; observable in a test tube; in an artificial environment.
In an artificial environment outside a living organism. investigations using human monocytes monocytes,
n.pl the largest of the white blood cells. They have one nucleus and a large amount of grayish-blue cytoplasm. Develop into macrophages and both consume foreign material and alert T cells to its presence. exposed to fine or coarse fractions of PM found an increased proinflammatory cytokine response with the coarse fraction but not with the fine fraction (Monn and Becker 1999). This cytokine induction was attributed to endotoxin Endotoxin
A biologically active substance produced by bacteria and consisting of lipopolysaccharide, a complex macromolecule containing a polysaccharide covalently linked to a unique lipid structure, termed lipid A. present only in coarse particles. In the present study, the increase in neutrophils in the lungs of animals exposed to CAPs is not likely due to endotoxins because they are not typically found at significant levels in ultrafine or fine particle size fractions.
Loss of membrane integrity is a late event in the process leading to decreased viability and cell death (Tyson and Green 1987). Cell viability has not been routinely measured to date in studies to examine the effects of PM on the respiratory system. However, one study reported viability of human monocytes after treatment with extracts of PM (Monn and Becker 1999). Cytotoxicity was increased in cells treated with the coarse fraction of PM. This cytotoxicity was attributed to transition metals. However, the water-insoluble fraction of PM was not used to treat monocytes in this study; therefore, cytotoxicity due to insoluble metals or other components was not determined. The decreased viability of BAL cells in our Fresno-based study may also be due to soluble or insoluble metals from ultrafine or fine CAPs. Transition metals in PM, such as iron, have been shown to catalyze the production of free radicals and lead to damage of biomolecules This page aims to list articles on Wikipedia that describe particular biomolecules or types of biomolecules.
This list is not necessarily complete or up to date - if you see an article that should be here but isn't (or one that shouldn't be here but is), please update the page (Donaldson et al 1997; Prahalad et al. 2001; Smith and Aust 1997).
The total concentration of individual chemical PM species during the fall season exposures was 88-96% of the total mass concentration measured gravimetrically, indicating a mass balance near 100%. During the last week of the winter season, the combined PM chemical species concentrations accounted for only 53% of the total PM mass, leaving 47% of the CAPs mass unexplained. The discrepancy in the mass balance during this week may have been due to the uncertainty associated with conversion of the measured OC concentration to the actual OC concentration. OC concentrations measured by thermal desorption must be multiplied by a conversion factor to obtain the mass of organic particulate compounds. A value of 1.4 has typically been accepted with this factor stemming from limited theoretical and laboratory studies (White and Roberts 1977). However, additional studies suggest that this factor may be as high as 2.6 (Schauer 1998; Turpin and Lim 2001). In all 6 weeks studied, measured OC concentrations were multiplied by a factor of 1.4 to maintain consistency. However, it is likely that seasonal changes in Fresno may have affected the value of this conversion factor. If the remaining unexplained portion of the mass concentration is attributable to OC, we believe that the actual OC concentration during the third week of the winter season could have been as high as 115 [micro]g/[m.sup.3]. This difficulty in accurately measuring constituents of PM makes correlating particle components with health effects more problematic. Because OC has been associated with significant health effects (Nel et al. 2001), this uncertainty in OC measurement will require further research to improve its accuracy in determination.
Associations between OC or endotoxin and biologic effects are only two of many associations made between particle components and health effects. Increased pulmonary neutrophils in dogs exposed to CAPs have been correlated with Al and Si in CAPs using factorial factorial
For any whole number, the product of all the counting numbers up to and including itself. It is indicated with an exclamation point: 4! (read “four factorial”) is 1 × 2 × 3 × 4 = 24. analysis (Clarke et al. 2000). Smith et al. (2000) used three different types of coal fly ash, with the same mean aerodynamic diameter but different concentrations of iron, to demonstrate that bioavailability bioavailability /bio·avail·a·bil·i·ty/ (bi?o-ah-val?ah-bil´i-te) the degree to which a drug or other substance becomes available to the target tissue after administration.
n. , rather than concentration of iron, was a predictor of proinflammatory cytokine induction in cultured human lung epithelial cells. Veranth et al. (2000) later determined that ferric ferric (fĕr`ĭk), iron in the +3 valence state.
See ferrous. iron associated with the aluminosilicate Aluminosilicate minerals are minerals composed of aluminum, silicon, and oxygen. Andalusite, kyanite, and sillimanite are naturally occuring aluminosilicate minerals that have the composition Al2SiO5. phase was the source of bioavailable iron in coal fly ash. It was further determined that bioavailable iron was associated with combustion particles but not with crustal dust derived from soil minerals (Veranth et al. 2000). These findings emphasize that knowledge of particle composition may not always allow accurate prediction of resultant health effects.
Table 3 suggests that the most consistent particle characteristic for six separate experiments in Fresno was particle number concentration. Concentrations of OC, CI, Ti, Fe, Zn, Mn, and Pb were also moderately consistent throughout the exposure period, with coefficients of variation less than 50%. In contrast, particle mass, as well as nitrate, sulfate, and EC concentrations, varied substantially over the 6-week period of exposure, with coefficients of variation ranging from 60% (for mass and sulfate) to 125% for EC. The concentrations of the remaining trace elements and metals (i.e., Al, Si, S, K, Ca, Ba, Ni, Cu, Se, Cd) also varied considerably, with coefficients of variation ranging from 50 to 250%.
Although each 3-day exposure over the 6 weeks of study varied in particle mass and composition, we were able to identify significant CAPs-induced respiratory effects in healthy adult rats produced by ambient particles in the Fresno area during both fall and winter seasons. It remains a challenge to assign specific biologic effects to a particular component or number of particles. However, additional studies in Fresno should help to determine if a consistent pattern of injury to the lung emerges as a result of exposure to well-characterized ambient particles based on particle size, composition, and season.
In summary, this study demonstrated that a) fine and ultrafine particles can be concentrated to study health effects; b) CAPs in Fresno produce respiratory change in healthy adult rats; c) respiratory changes could be measured during both the fall and winter seasons in Fresno; and d) respiratory changes based on cell viability are independent of mean particle mass concentration during these 3-day periods of exposure.
Figure 3. Chemical composition of CAPs during the first (A), second (B), and third (C) weeks of fall 2000 exposures. Numbers represent the average mass concentration ([micro]g/[m.sup.3]) of each species measured over the 3-day period of exposure for each experiment. The unexplained fraction (Unexpl) represents that portion of the total particle mass not accounted for by chemical analysis. Week 1 [A] Nitrate (527) Sulfate (49) Metals (38) Unexpl (33) EC (59) OC (141) Week 2 [B] Nitrate (63) Sulfate (19) Metals (42) Unexpl (32) EC (9) OC (95) Week 3 [C] Nitrate (211) Sulfate (26) Metals (14) Unexpl (30) EC (8) OC (80) Note: Table made from pie graph. Figure 5. Chemical composition of CAPs during the first (A), second (B), and third (C) weeks of winter 2001 exposures. Numbers represent that average mass concentration ([micro]g/[m.sup.3]) of each species measured over the 3-day period of exposure for each experiment. The unexplained fraction (Unexpl) represents that portion of the total particle mass not accounted for by chemical analysis. Week 1 [A] Nitrate (433) Sulfate (51) Metals (16) Unexpl (171) EC (15) OC (129) Week 2 [B] Nitrate (58) Sulfate (13) Metals (14) Unexpl (41) EC (4) OC (61) Week 3 [C] Nitrate (108) Sulfate (14) Metals (8) Unexpl (174) EC (6) OC (61) Note: Table made from pie graph. Table 1. Experimental design showing study designation, exposure dates, age of animals, and number of rats used per group. Study designation CAPs exposure dates First week of fall 17-19 October 2000 Second week of fall 24-26 October 2000 Third week of fall 31 October-2 November 2000 First week of winter 30 January-1 February 2001 Second week of winter 6-8 February 2001 Third week of winter 13-15 February 2001 No. of rats exposed Study designation Age (weeks) (a) Filtered air CAPs First week of fall 10 6 6 Second week of fall 9 6 6 Third week of fall 9 6 6 First week of winter 9 6 6 Second week of winter 9 6 6 Third week of winter 9 6 6 (a) Age of rats at beginning of exposure. Table 2. Mass and number concentrations of CAPs. Mass Number concentration concentration Study ([micro]g/[m.sup.3]) (particles/[cm.sup.3]) Fall 2000 First week 847 1.2 x [10.sup.5] Second week 260 1.2 x [10.sup.5] Third week 369 1.1 x [10.sup.5] Winter 2001 First week 815 0.9 x [10.sup.5] Second week 190 1.0 x [10.sup.5] Third week 371 1.2 x [10.sup.5] Table 3. CAPs means and coefficients of variation for 6 weeks of exposures in Fresno, California. Arithmetic mean CAPs species concentration Coefficient of and elements ([micro]g/[m.sup.3]) (a) variation Number 1.10 x [10.sup.5] (b) 13 Mass 475 60 0C 94.5 36 EC 16.7 125 Nitrate 233 86 Sulfate 28.7 60 Al 3.0 83 Si 8.3 69 S 6.7 63 Cl 1.8 39 K 2.2 50 Ca 2.4 56 Ti 0.26 45 Fe 3.3 49 Zn 0.54 46 Mn 0.08 46 Ba 0.13 200 Ni 13 (c) 63 Cu 130 (c) 65 Cd 11.2 (c) 245 Se 9.6 (c) 136 Pb 91 (c) 36 (a) Arithmetic mean concentrations shown are in [micro]g/[m.sup.3] except where indicated. (b) Concentrations are expressed in particles/[cm.sup.3]. (c) Concentrations are expressed in ng/[m.sup.3]. Table 4. BAL fluid cell differentials. (a) Percent macrophages Study Filtered air CAPs Fall 2000 First week 99.7 [+ or -] 0.2 99.2 [+ or -] 0.1 Second week 99.5 [+ or -] 0.1 98.8 [+ or -] 0.5 (b) Third week 99.6 [+ or -] 0.1 99.7 [+ or -] 0.1 Winter 2001 First week 99.3 [+ or -] 0.3 99.3 [+ or -] 0.1 Second week 99.7 [+ or -] 0.1 99.6 [+ or -] 0.1 Third week 99.6 [+ or -] 0.1 99.3 [+ or -] 0.2 Percent neutrophils Study Filtered air CAPs Fall 2000 First week 0.3 [+ or -] 0.2 0.7 [+ or -] 0.1 Second week 0.4 [+ or -] 0.1 0.9 [+ or -] 0.4 Third week 0.3 [+ or -] 0.1 0.2 [+ or -] 0.0 Winter 2001 First week 0.3 [+ or -] 0.0 0.6 [+ or -] 0.1 (b) Second week 0.2 [+ or -] 0.1 0.4 [+ or -] 0.1 Third week 0.3 [+ or -] 0.1 0.5 [+ or -] 0.1 Percent lymphocytes Study Filtered air CAPs Fall 2000 First week 0.1 [+ or -] 0.0 0.1 [+ or -] 0.1 Second week 0.2 [+ or -] 0.0 0.3 [+ or -] 0.1 Third week 0.2 [+ or -] 0.0 0.2 [+ or -] 0.1 Winter 2001 First week 0.1 [+ or -] 0.0 0.1 [+ or -] 0.0 Second week 0.0 [+ or -] 0.0 0.1 [+ or -] 0.0 Third week 0.1 [+ or -] 0.1 0.2 [+ or -] 0.1 (a) Data are presented as mean [+ or -] SE (n = 6). (b) Significantly different compared with filtered air (p < 0.05).
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Formation of new and distinct species, whereby a single evolutionary line splits into two or more genetically independent ones. One of the fundamental processes of evolution, speciation may occur in many ways. by Mn[O.sub.2] oxidation. Aerosol Sci Technol 12:122-127.
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1. A waterfall.
2. A steep ravine.
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Within a living organism.
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Foreign to the body or to living organisms. Used of chemical compounds.
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White WH, Roberts PT. 1977. On the nature and origins of visibility reducing aerosols in the Los Angeles Basin The Los Angeles Basin is the coastal sediment-filled plain located between the peninsular and transverse ranges in southern California in the United States containing the central part of the city of Los Angeles as well as its southern and southeastern suburbs (both in Los Angeles . Atmos Environ 11:803-812.
Kevin R. Smith, (1) Seongheon Kim, (2) Julian J. Recendez, (1) Stephen V. Teague, (1) Margaret G. Menache, (3) David E. Grubbs, (4) Constantinos Sioutas, (2) and Kent E. Pinkerton (1)
(1) Center for Health and the Environment, University of California, Davis, California, USA; (2) Civil and Environmental Engineering, University of Southern California, Los Angeles, California, USA; (3) Department of Pediatrics, University of New Mexico The University of New Mexico (UNM) is a public university in Albuquerque, New Mexico. It was founded in 1889. It also offers multiple bachelor's, master's, doctoral, and professional degree programs in all areas of the arts, sciences, and engineering. , Albuquerque, New Mexico “Albuquerque” redirects here. For other uses, see Albuquerque (disambiguation).
Albuquerque (pronounced [ˈæl.bə.kɚ.kiː], Spanish: [al.βu. , USA; (4) Department of Biology, College of Science and Mathematics, California State University, Fresno, California, USA
Address correspondence to K.R. Smith, Center for Health and the Environment, University of California The University of California has a combined student body of more than 191,000 students, over 1,340,000 living alumni, and a combined systemwide and campus endowment of just over $7.3 billion (8th largest in the United States). , One Shields Ave., Davis, CA 95616 USA. Telephone: (530) 752-5121. Fax: (530) 752-5300. E-mail: email@example.com
We gratefully acknowledge support of faculty and staff at California State University, Fresno, with special thanks to K. Jones and K.P. Wong in the Dean's Office of the School of Science and Mathematics.
This study was supported in part by grants from the U.S. Environmental Protection Agency (R827995 and R829215), the National Institutes of Health (ES05707), and the Western Center for Agricultural Health and Safety (NIOSH NIOSH National Institute for Occupational Safety & Health, see there
NIOSH Recommendations for Safety & Health Standards
Agent NIOSH REL*/OSHA PEL† Health effects OH07550).
The authors declare they have no conflict of interest.
Received 29 August 2002; accepted 3 February 2003.