Aintree '99: Eudipe team left stunned by loss.
One of more fancied runners at 10-1, Eudipe had worked his way into a challenging position on the final circuit and was just behind the leading bunch when crashing out at Becher's Brook.
At the same fence, Camelot Knight, Fiddling the Facts and Frazer Island fell, while Choisty was brought down.
Like Gris d'Estruval, who broke a leg while running in Friday's John Hughes, Eudipe represented the championship-winning team of trainer Martin Pipe, jockey Tony McCoy and owner David Johnson.
Johnson said: "I honestly can't believe it-two in two days is too much for anyone.
"I haven't got anything against Aintree-we had a wonderful success here with Cyfor Malta 12 months ago-but I'm not sure if it's for me from now on.
"We lost another nice horse here a couple of years ago, and it doesn't seem a lucky course despite Cyfor's win. I can't see us having another runner over the Aintree fences."
Eudipe ran 18 times, winning on five occasions and earning nearly pounds 140,000 in prize-money with his biggest success in the Mildmay Cazalet Memorial at Sandown this season.
Pipe added: "Everyone is very upset, none more than the lasses who looked after Gris d'Estruval and Eudipe.
"Delphine Guilla cared for Eudipe and Donna Cornforth-Smith Gris d'Estruval. Both have been in tears-they are the ones who look after the horses every day."
Meanwhile, owners The Winning Line lost Stretarez after he was put down having fractured his right fore cannon bone in the Martell Aintree Hurdle. The injury came as another blow to The Winning Line, whose star chaser Teeton Mill suffered a career-threatening injury in the Tote Cheltenham Gold Cup.