Agilent Technologies Enables Peptide Mapping of Antibodies Up to 20X Faster than Traditional Methods.PALO ALTO Palo Alto, city, California Palo Alto (păl`ō ăl`tō), city (1990 pop. 55,900), Santa Clara co., W Calif.; inc. 1894. Although primarily residential, Palo Alto has aerospace, electronics, and advanced research industries. , Calif. -- Agilent Technologies This article needs sources or references that appear in reliable, third-party publications. Alone, primary sources and sources affiliated with the subject of this article are not sufficient for an accurate encyclopedia article. Inc. (NYSE NYSE See: New York Stock Exchange :A) today announced a liquid chromatography (LC) application for separating and identifying protein fragments that is up to 20 times faster than typical methods. The application dramatically reduces the cost and time required for high-resolution peptide mapping of antibodies, an important class of proteins used in general research, diagnostic and therapeutic applications. Peptide mapping is a technique for analyzing proteins by separating and detecting the mixture of fragments (peptides) generated when a protein is broken up with chemicals or enzymes. The analysis can provide information about the original protein's sequence and detect subtle differences between proteins when coupled with mass spectrometry mass spectrometry or mass spectroscopy Analytic technique by which chemical substances are identified by sorting gaseous ions by mass using electric and magnetic fields. . Most peptide mapping methods rely on traditional LC columns containing totally porous porous /por·ous/ (por´us) penetrated by pores and open spaces. po·rous adj. 1. Full of or having pores. 2. Admitting the passage of gas or liquid through pores. particles. Agilent's application uses an LC column packed with Poroshell, a superficially porous chromatographic chro·mat·o·graph n. An instrument that produces a chromatogram. tr.v. chro·mat·o·graphed, chro·mat·o·graph·ing, chro·mat·o·graphs To separate and analyze by chromatography. media, to dramatically reduce the time required for peptide mapping of human monoclonal antibodies This is a list of monoclonal antibodies, antibodies which are clones of a single parent cell. When used as medications, the generic names end in -mab (see "Nomenclature of monoclonal antibodies"). . Compared to traditional methods, the new application reduces analysis time from 120 minutes to 20.5 minutes, achieving a six-fold increase in speed without a loss in resolution. For ultra-high-speed peptide mapping, the application enables run times as low as 5.6 minutes, a 20-fold increase in speed, with a resolution loss of 18 to 19 percent. The impact of this resolution loss is minimal, however, when using a sensitive, discriminating detector such as a mass spectrometer spectrometer Device for detecting and analyzing wavelengths of electromagnetic radiation, commonly used for molecular spectroscopy; more broadly, any of various instruments in which an emission (as of electromagnetic radiation or particles) is spread out according to some . Available in Agilent ZORBAX Poroshell 300SB columns, Poroshell packing technology uses particles consisting of a solid silica core surrounded by a thin outer layer of porous silica. This innovative design allows for higher throughput and flow rates while maintaining sharp peak shape and excellent resolution. During separation, proteins and peptides rapidly diffuse into and out of the thin porous shell and elute e·lute tr.v. e·lut·ed, e·lut·ing, e·lutes To extract (one material) from another, usually by means of a solvent. [From Latin in seconds as easily resolved, narrow bands. Agilent ZORBAX Poroshell 300SB columns are available in a variety of internal diameters and bonded phases. Further information is available by requesting Agilent application note "High Speed and Ultra-High-Speed Peptide Mapping of Human Monoclonal IgG on ZORBAX Poroshell 300SB-C18, C8, and C3," 5989-0590EN. This note is available without charge from any Agilent sales office or at www.agilent.com/chem. About Agilent Technologies Agilent Technologies Inc. (NYSE:A) is a global technology leader in communications, electronics, life sciences and chemical analysis. The company's 28,000 employees serve customers in more than 110 countries. Agilent had net revenue of $6.1 billion in fiscal year 2003. Information about Agilent is available on the Web at www.agilent.com. NOTE TO EDITORS: Press releases, photography and other information can be accessed on the Agilent Life Sciences and Chemical Analysis newsroom at www.agilent.com/about/newsroom/lsca. If you choose to review this item, your readers will receive the quickest response to their inquiries by e-mailing the inquiries, with reference number MC7550, to carol_dunn@agilent.com. Alternatively, readers can mail inquiries to Agilent Technologies, Inc., 71 Southgate Boulevard, no. MC7550, New Castle, DE 19720, or call 1-800-227-9770. Further technology, corporate citizenship Corporate Citizenship The extent to which businesses are socially responsible in meeting legal, ethical and economic responsibilities placed on them by shareholders. The aim it to create higher standards of living and quality of life in the community in which it operates, while and executive news is available on the Agilent news site at www.agilent.com/go/news. |
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