ASSESSMENT OF THE ROLE OF G-PROTEINS IN REGULATION OF ECDYSTEROIDOGENESIS IN BLUE CRAB (CALLINECTES SAPIDUS) Y-ORGANS.ASSESSMENT OF THE ROLE OF G-PROTEINS IN REGULATION OF ECDYSTEROIDOGENESIS IN BLUE CRAB (CALLINECTES SAPIDUS) Y-ORGANS. Deug Woo Han and R. Douglas Watson, Department of Biology, University of Alabama at Birmingham UAB began in 1936 as the Birmingham Extension Center of the University of Alabama. Because of the rapid growth of the Birmingham area, it was decided that an extension program for students who had difficulties which prevented them from studying in Tuscaloosa was needed. , AL 35294. In decapod decapod (dĕk`əpŏd') (Gr.,=10 feet), name for invertebrate animals of the crustacean order Decapoda (phylum Arthropoda) including the crabs, the lobsters and crayfish, and the true shrimps, all having five pairs of legs. crustaceans, a neuropeptide neuropeptide /neu·ro·pep·tide/ (noor?o-pep´tid) any of the molecules composed of short chains of amino acids (endorphins, enkephalins, vasopressin, etc.) found in brain tissue. neu·ro·pep·tide n. molt-inhibiting hormone (MIH MIH Make It Happen (band) MIH Master of International Health (University of Copenhagen) MIH Molar-Incisor-Hypomineralisation MIH Men in Hats (online comic) ) suppresses ecdysteroids synthesis by Y-organs. Cyclic AMP has been implicated as a second messenger in MIH action. Since heterotrimeric Gs-proteins are known to be involved in cAMP-mediated responses, we assessed the presence of Gs-proteins in blue crab (Callinectes sapidus) Y-organs. Cholera toxin-catalyzed ADP-ribosylation did not reveal Gs-like proteins in Y-organs. However, immunoblot analysis, using antibodies raised against vertebrate Gs revealed an immunoreactive immunoreactive exhibiting immunoreactivity. band of the predicted size ([sim]49kD) in Y-organ and control tissue (blue crab thoracic ganglion and mouse brain). The results indicate that Gs-like proteins are present in blue crab Y-organs. We are currently investigating the functional link of G-protein activation to regulation of ecdysteroidogenesis. Funded by MS/AL Sea Grant (NA86RG0039). |
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