ADVISORY/Linden Bioscience to Present at BioTechniques Live.Business Editors/Health/Medical Writers ADVISORY...for Wednesday (March 5) --(BUSINESS WIRE)--March 5, 2003 Linden Bioscience
Who: Simon W. Law, Ph.D., Director of Biology
What: "A New Archivable Subnano Scale Nucleic Acid
Amplification Method"
Where: World Trade Center Boston
When: Wednesday, March 5, 2003, 2:00 p.m. - 7:00 p.m. EST
(during Science Fair)
Recent advances in DNA microarray DNA microarray A small solid support, usually a membrane or glass slide, on which sequences of DNA are fixed in an orderly arrangement. DNA microarrays are used for rapid surveys of the expression of many genes simultaneously, as the sequences contained on a technologies have made high throughput gene expression and other genomic studies possible. To conduct global expression studies, investigators need a sufficient quantity of sample to do a statistically meaningful experiment. A typical glass slide cDNA or oligoarray analysis requires 30-50 mg total RNA RNA: see nucleic acid. RNA in full ribonucleic acid One of the two main types of nucleic acid (the other being DNA), which functions in cellular protein synthesis in all living cells and replaces DNA as the carrier of genetic per slide. However, disease tissues are usually heterogeneous and sample complexity limits the type of questions that might be addressed. Samples obtained by laser-capture microdissection (LCM (Liquid Crystal Monitor) A flat panel display that uses the liquid crystal (LCD) technology. See flat panel display. ) are more homogeneous, but contain very few cells. The transcription-based method is preferred over other nucleic acid nucleic acid, any of a group of organic substances found in the chromosomes of living cells and viruses that play a central role in the storage and replication of hereditary information and in the expression of this information through protein synthesis. amplification methods because the original mRNA profile of the sample is better preserved. Recently, a few linear amplification methods, which are modifications of the original Eberwine T7 RNA polymerase T7 RNA Polymerase is an RNA polymerase that catalyzes the formation of RNA in the 5'→ 3' direction. T7 RNA polymerase is extremely promoter-specific and only transcribes bacteriophage T7 DNA or DNA cloned downstream of a T7 promoter. transcription procedure, have been developed to address small sample sizes such as those derived from laser-capture microdissection (LCM). However, the amount of RNA obtained from these samples is not enough for multiple analyses. Moreover, all these methods are solution based and the original sample can only be used once. Therefore, there is a need for a more powerful, transcription-based amplification method to address samples of 100 cells and below. In the poster being presented, Dr. Law describes the development of a new amplification method, solid phase transcription chain reaction, which can amplify subnanogram target nucleic acids Nucleic acids The cellular molecules DNA and RNA that act as coded instructions for the production of proteins and are copied for transmission of inherited traits. to more than one billion fold. In addition, the original sample is permanently archived to permit downstream characterization and processing. This method should be useful for gene expression analysis as well as broad molecular biology molecular biology, scientific study of the molecular basis of life processes, including cellular respiration, excretion, and reproduction. The term molecular biology was coined in 1938 by Warren Weaver, then director of the natural sciences program at the Rockefeller applications. About Linden Bioscience Linden Bioscience rapidly integrates cutting-edge technology in functional genomics Noun 1. functional genomics - the branch of genomics that determines the biological function of the genes and their products genomics - the branch of genetics that studies organisms in terms of their genomes (their full DNA sequences) , proteomics, bioinformatics and computational biology into an Applied Systems Biology-based technology platform. The company uses this platform to discover and validate disease biomarkers, targets and therapeutics. Through its Applied Systems Biology approach, the company's vision is to - on its own and with partners - efficiently develop drugs with the potential for greater efficacy and safety as compared to those developed using traditional drug development processes. Additional information may be found at http://www.lindenbioscience.com. |
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